O microplancton do Atlantico nas imediações de Mar del Plata

Agatha

J Eukaryotic Microbiology

et al. 2016

To improve understanding of diversity, phylogeny and evolution in tintinnid ciliates, it is essential to link multiple molecular markers with properly identified and documented morphospecies. Accordingly, 54 tintinnid morphospecies/isolates mainly from the Yellow and East China Seas were collected and analysed. Using single‐cell approaches, sequences were obtained for three rDNA loci (18S, ITS1‐5.8S‐ITS2, D1‐D5 region of 28S). Twenty‐six tintinnid morphospecies (29 isolates) are documented by micrographs, measurements, morphologically described, and compared with the original species description. Three rDNA loci‐based phylogenetic analyses were then performed for these identified isolates. Sequences from 25 unidentified species/isolates were also included in the comparison of the three rDNA loci. Ribosomal DNA genes of the genus Leprotintinnus were analysed for the first time, showing that Leprotintinnus was closely related to Tintinnopsis radix and branched distinctly apart from the family Tintinnidiidae. Four novel clades (VI to IX) of the Tintinnopsis complex emerged in the 18S genealogies. Analyses of the relative variability in the ITS and 28S regions vs. the 18S rDNA showed that the ITS1‐5.8S‐ITS2 and ITS2 regions well co‐varied with the 18S rDNA when the variations of the latter were less than 3%, whereas at difference of less than 1%, no correlation was found between the compared loci. These findings highlight the difficulties in using variable locus‐based cut‐off divergences in circumscribing tintinnid morphospecies.

Section: Discussionmentioning

confidence: 99%

Three rDNA Loci‐Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)

Agatha

J Eukaryotic Microbiology

et al. 2016

“…Comentários: a presença de colar tem sido motivo de discussão. Balech (1962) informa que ele sempre ocorreu em seus exemplares, embora Kofoid & Campbell (1939) Cunha & Fonseca, 1917:141 $O\.1to, 1970a Descrição: lórica aglutinante cilíndrica, tornando-se cônica na região abora!. Região oral lisa e irregular; região aboral de lados convergentes e convexos, formando uma extremidade aboral obtusa ou acuminada, encimada por um apêndice caudal hia.linoafilÇl,do, cônico e agudo.…”

Section: Q-230llm;unclassified

Tintininos (Ciliophora - 5ubordem Tintinnina) de águas subantárticas e antárticas entre a Argentina e a Península Antártica (35º8-62º8) (Novembro de 1992)

Fernandes

1999

Rev. Bras. Oceanogr.

Um estudo taxonômico sobre os tintininos (Subordem Tintinnina) foi realizado a partir de 58 amostras de água de superfície coletadas em novembro de 1992 ao longo de um transecto latitudinal entre a Argentina e a Península Antártica (35ºS-62ºS). Foram determinados 22 táxons, pertencentes aos gêneros Acanthostomella, Amphorides, Codonellopsis, Coxliella, Cymatocylis, Favella, Helicostomella, Laackmaniella, Protorhabdonella, Stylicauda e Tintinnopsis. As espécies mais abundantes foram Acanthostomella norvegica, Amphorides quadrilineata, Codonellopsis gaussii, Cymatocylis antarctica, C. convalaria e Tintinnopsis bacillaria. A espécie C. antarctica dominou em quase todas as estações amostradas em águas subantárticas, enquanto que C. convalaria e Codonellopsis gaussi foram mais abundantes em águas antárticas. Os gêneros Favella, Helicostomella, Stylicauda e a espécie Tintinnopsis gracilis ocorreram exclusivamente em águas neríticas ou intluenciadas pelo Rio da Prata. Para todos os táxons são fornecidos descrições, medidas e desenhos, bem como comentários sobre sua taxonomia.

J Eukaryotic Microbiology

“…In the future, with a more comprehensive knowledge about tintinnid morphology, specifically cell features, these monophyletic clades will receive a systematic rank. Therefore, the genera Rhizodomus and Stylicauda are maintained here and in Agatha and Str€ uder-Kypke (2013), although some authors discuss synonymisation with the genus Tintinnopsis (da Cunha and da Fonseca 1917;Laval-Peuto 1994).…”

mentioning

confidence: 99%

Redescription of Tintinnopsis everta Kofoid and Campbell 1929 (Alveolata, Ciliophora, Tintinnina) Based on Taxonomic and Genetic Analyses—Discovery of a New Complex Ciliary Pattern

Gruber

Strüder‐Kypke

2018

The about 1,000 species of tintinnid ciliates are identified and classified almost exclusively based on their lorica features, although the shortcomings of this structure are well‐known, e.g. causing uncertain species limitations and nonmonophyletic taxa. Hence, the present redescription of Tintinnopsis everta Kofoid and Campbell, 1929 considers not only the lorica characteristics, but focuses on cell and genetic features. The species is redescribed from the North Atlantic and adjacent sea areas, namely the east coast of the USA, using live observation, protargol‐stained material, scanning electron microscopy, and genetic analyses. The main stages of cell division are described, and the species’ phylogenetic relationships are inferred from morphological data and the small subunit ribosomal RNA gene sequence. The estimates of its biogeographical distribution and autecology are based on a literature survey. The species is characterised by a complex somatic ciliary pattern with a unique position of the posterior kinety and a conspicuously large distance between the somatic ciliary fields and the collar membranelles. The phylogenetic relationships of Tintinnopsis everta vary in the molecular trees depending on the algorithms used and are, therefore, regarded as unresolved. Nevertheless, the new kind of complex somatic ciliary pattern distinctly contributes to a better understanding of the tintinnid biodiversity and evolution and provides features for a future split of the nonmonophyletic genus Tintinnopsis.