2013
DOI: 10.1590/s0074-0276108042013016
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Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

Abstract: In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthe… Show more

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Cited by 21 publications
(16 citation statements)
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“…This suggests that uPE and uPC (to lesser extent) play integral roles in the determination of crystal morphology for hemozoin in the midgut of R. prolixus . This is in agreement with previous findings, which reported 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine as the major phospholipid synthesized by R. prolixus midgut epithelium (Bittencourt-Cunha et al, 2013 ). A similar involvement of lipids in hemozoin formation has been well documented in the parasites Plasmodium and Schistosoma mansoni (blood fluke) (Oliveira et al, 2004 ; Corrêa Soares et al, 2007 ; Stiebler et al, 2010 , 2014 ).…”
Section: Protective Adaptations Against Oxidative Stress After a Bloosupporting
confidence: 94%
“…This suggests that uPE and uPC (to lesser extent) play integral roles in the determination of crystal morphology for hemozoin in the midgut of R. prolixus . This is in agreement with previous findings, which reported 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine as the major phospholipid synthesized by R. prolixus midgut epithelium (Bittencourt-Cunha et al, 2013 ). A similar involvement of lipids in hemozoin formation has been well documented in the parasites Plasmodium and Schistosoma mansoni (blood fluke) (Oliveira et al, 2004 ; Corrêa Soares et al, 2007 ; Stiebler et al, 2010 , 2014 ).…”
Section: Protective Adaptations Against Oxidative Stress After a Bloosupporting
confidence: 94%
“…Another possibility to consider is that these two kinetically distinct components of uPC were related to the way by which heme interacts with the exposed CH 3 groups at the surface of uPC vesicles [46]. Interestingly, β-hematin formation induced by the phospholipid blend was revealed to be as fast as the uPE-mediated heme crystallization, suggesting that beyond representing the dominant phospholipid synthesized by R. prolixus midgut epithelia [52] uPE is able to produce crystals very similar in shape to those found biologically (Figure 2), mediating fast and efficient β-hematin formation (about 60% of heme conversion) (Figure 4). Regardless of the catalyst, we can observe that all reactions were essentially completed after 8 h (∼500 minutes) and all exhibited two kinetically distinct components: one very fast, making a major contribution to β-hematin conversion (54% to 100%), and the other a slow one.…”
Section: Resultsmentioning
confidence: 99%
“…In the midgut of the triatomine insect Rhodnius prolixus , Hz formation is mediated by perimicrovillar membranes (PMVM) [34] , phospholipid bilayers that cover the epithelial cells of the midgut [50] , [51] , with fewer integral proteins [15] , [16] and with phosphaditylethanolamine (PE) being the most abundant phospholipid synthesized by the midgut [52] . Hz crystals have also been observed in close association with PMVM [35] , [20] .…”
Section: Introductionmentioning
confidence: 99%
“…This structure also allows anchoring of digestive enzymes [65]. It is likely that RhoprACSL2 activates and channels dietary fatty acids into pathways of intracellular PL synthesis that constitute the perimicrovillar membranes [66], thereby directly affecting digestion rates.…”
Section: Discussionmentioning
confidence: 99%