Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Moreira, Rodrigo de Carvalho; Haddad, Andrea Ferreira; Silva, Suzana Alves; Souza, André Luis Santos de; Tuche, Fabio; Oliveira, Monica Amorim de; Mesquita, Cláudio Tinoco; Rochitte, Carlos Eduardo; Borojević, Radovan; Dohmann, Hans Fernando Rocha

doi:10.1590/s0066-782x2011005000095

Cited by 20 publications

(6 citation statements)

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“…Its feasibility and safety have been demonstrated clinically and experimentally [1-6]. However, the efficiency of retrograde delivery into the heart was considered the lowest when compared with antegrade coronary and intramyocardial injection-based delivery techniques [7-9]. Poor cell retention has been the main hurdle in establishing retrograde coronary infusion as the preferred route for cell delivery.…”

Section: Discussionmentioning

confidence: 99%

“…With unique access to the ischemic myocardium, retrograde coronary venous delivery has been demonstrated to provide efficient cell dissemination in the setting of occluded or diffusely narrowed coronary arteries and has subsequently shown functional benefits in both animal and clinical studies [1-6]. However, compared with the antegrade approach, cell retention using the retrograde intracoronary approach was inferior [7-9]. Poor cell retention is the major obstacle in establishing this method as the preferred route for cell delivery.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction

et al. 2013

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IntroductionRetrograde coronary venous infusion is a promising delivery method for cellular cardiomyoplasty. Poor cell retention is the major obstacle to the establishment of this method as the preferred route for cell delivery. Here, we explored whether magnetic targeting could enhance retrograde cell retention in a rat model of myocardial infarction.MethodsRat mesenchymal stem cells were labeled with superparamagnetic oxide nanoparticles. The magnetic responsiveness of MSCs was observed while cells flowed through a tube that served as a model of blood vessels in a 0.6-Tesla magnetic field. In a Sprague–Dawley rat model of acute myocardial infarction, 1 × 106 magnetic mesenchymal stem cells were transjugularly injected into the left cardiac vein while a 0.6-Tesla magnet was placed above the heart. The cardiac retention of transplanted cells was assessed by using quantitative Y chromosome-specific polymerase chain reaction, cardiac magnetic resonance imaging, and optical imaging. Cardiac function was measured by using echocardiography, and histologic analyses of infarct morphology and angiogenesis were obtained.ResultsThe flowing iron oxide-labeled mesenchymal stem cells were effectively attracted to the area where the magnet was positioned. Twenty-four hours after cellular retrocoronary delivery, magnetic targeting significantly increased the cardiac retention of transplanted cells by 2.73- to 2.87-fold. Histologic analyses showed that more transplanted cells were distributed in the anterior wall of the left ventricle. The enhanced cell engraftment persisted for at least 3 weeks, at which time, left ventricular remodeling was attenuated, and cardiac function benefit was improved.ConclusionsThese results suggest that magnetic targeting offers new perspectives for retrograde coronary venous delivery to enhance cell retention and subsequent functional benefit in heart diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction

et al. 2013

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“…The remaining 68 articles were screened on the availability of full text, leading to another 42 exclusions. One article was excluded due to a shared dataset [ 8 ]. The cross-reference screening led to one additional inclusion that did not come up in the original search due to the absence of one part of the search string in the title and abstract [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

Retrograde Coronary Venous Infusion as a Delivery Strategy in Regenerative Cardiac Therapy: an Overview of Preclinical and Clinical Data

Gathier

Ginkel

Naald

et al. 2018

J. of Cardiovasc. Trans. Res.

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An important aspect of cell therapy in the field of cardiac disease is safe and effective delivery of cells. Commonly used delivery strategies such as intramyocardial injection and intracoronary infusion both present with advantages and disadvantages. Therefore, alternative delivery routes are explored, such as retrograde coronary venous infusion (RCVI). Our aim is to evaluate safety and efficiency of RCVI by providing a complete overview of preclinical and clinical studies applying RCVI in a broad range of disease types and experimental models. Available data on technical and safety aspects of RCVI are incomplete and insufficient. Improvement of cardiac function is seen after cell delivery via RCVI. However, cell retention in the heart after RCVI appears inferior compared to intracoronary infusion and intramyocardial injection. Adequately powered confirmatory studies on retention rates and safety are needed to proceed with RCVI in the future.Electronic supplementary materialThe online version of this article (10.1007/s12265-018-9785-1) contains supplementary material, which is available to authorized users.

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“…Porém, os resultados destas aplicações clínicas ainda são desconhecidos não perdendo seu potencial, mas não deixam de serem promissores e na maioria dos casos mostram um percentual regenerativo importante para pacientes submetidos à terapia (JACOB et al, 2007). Moreira et al (2011) descreveram o uso de injeção de CTs no Infarto Agudo do Miocárdio (IAM) sendo este um teste, quase exclusivo, da via anterógrada Intra-Arterial Coronariana (IAC). Também foi observado no trabalho que a via retrógrada intravenosa coronariana pode ser uma via adicional.…”

Section: Uso Clínico Na Cardiologiaunclassified

Uso De Terapia Celular Com Células-Tronco Nas Especialidades Médicas No Brasil: Revisão Sistemática

Barbosa¹,

Menezes²,

Emanuelli³

2018

Rev. Uningá

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O uso de células-tronco na medicina demonstra inúmeras possibilidades terapêuticas, entretanto, não há resultados a longo prazo destes tratamentos até o momento, gerando incerteza quanto à sua utilização, tanto na comunidade médica e para os pacientes. Um estudo que apresente sistematicamente o estado da arte do uso clínico destas células seria esclarecedor tanto para o meio médico quanto para os pacientes. Diante disso, este trabalho tem por objetivo descrever as aplicações clínicas disponíveis no Brasil para o uso de células-tronco em diferentes especialidades médicas. Realizou-se uma revisão sistemática de caráter exploratório da literatura presente nas bases de dados Lilacs, Ibecs, Medline e Scielo, com os descritores Células-tronco, Medicina regenerativa, Terapia baseada em transplante de células e tecidos e Transplante de células-tronco. Foram selecionados 20 artigos de acordo com o cumprimento do objetivo e critérios metodológicos, abrangendo ás áreas médicas da hematologia, cardiologia, oftalmologia, reumatologia, ortopedia, cirurgia vascular e dermatologia. Conclui-se que o tratamento com células-tronco é empregado de forma definitiva ou adjuvante a outras terapias nas áreas médicas supracitadas, diminuindo em alguns casos o emprego de terapias mais agressivas ou a minimização dos efeitos colaterais associados a estas, além de trazer resultados que não poderiam ser conseguidos anteriormente.

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Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Abstract: Background: The injection of stem cells in the context of acute myocardial infarction (AMI) has been tested almost exclusively by anterograde intra-arterial coronary (IAC) delivery. The retrograde intravenous coronary (IVC) delivery may be an additional route.

Cited by 20 publications

References 21 publications

Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction

Magnetic targeting enhances retrograde cell retention in a rat model of myocardial infarction

Retrograde Coronary Venous Infusion as a Delivery Strategy in Regenerative Cardiac Therapy: an Overview of Preclinical and Clinical Data

Uso De Terapia Celular Com Células-Tronco Nas Especialidades Médicas No Brasil: Revisão Sistemática

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