Amplificação dos genes que codificam a endotelina-1 e seus receptores em valvas mitrais reumáticas

Moura, Edmilson Bastos de; Gomes, Mariana Ribeiro; Corso, Ricardo Barros; Faber, Cristiano N.; Carneiro, Fabiana Pirani; Pacheco, Yolanda Galindo

doi:10.1590/s0066-782x2010005000059

Cited by 4 publications

(6 citation statements)

References 9 publications

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“…In our study, there is gene expression of endothelin-1 in damaged heart valves in patients that underwent mitral valve replacement, resembling those seen in other samples of the Brazilian population. In this sense, Moura et al [ 1 ] found that 40.7% of mitral valves (fibrosed and stenosed) replaced in patients with RF presented gene expression of ET-1 and, in our previous study [ 22 ] , we observed expression of both endothelin receptors (ETrA and ETrB) in replaced rheumatic mitral valves.…”

Section: Discussionsupporting

confidence: 54%

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IL-10 and ET-1 as biomarkers of rheumatic valve disease

Leão¹,

Lima²,

Nascimento³

et al. 2014

Revista Brasileira De Cirurgia Cardiovascular

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Objective: To evaluate the immunological profile and gene expression of endothelin-1 (ET-1) in mitral valves of patients with rheumatic fever originated from a reference service in cardiovascular surgery.Methods: This was a quantitative, observational and cross-sectional study. Thirty-five subjects (divided into four groups) participated in the study, 25 patients with chronic rheumatic heart disease and ten control subjects. The mean age of the sample studied was 34.5 years. Seventeen of them (48.58%) were male and 18 (51.42%) were female. Inflammatory cytokines (TNF-α, IL-4 and IL-10) were measured and ten mitral valves of patients who underwent first valve replacement were collected for determination of gene expression of endothelin-1 by real time PCR.Results: Among the groups studied (patients vs. controls), there was a statistically significant difference in IL-10 levels (P=0.002), and no differences in other cytokines. Expression of endothelin-1 was observed in 70% of samples. Quantitatively, average of ET-1 expression was 62.85±25.63%.Conclusion: Inflammatory cytokine IL-10 participates in the maintenance of chronicity of rheumatic fever in patients who underwent valve replacement and those who are undergoing medical treatment. The expression of endothelin-1 in heart valve lesions in patients undergoing mitral valve replacement confirms its association with inflammatory activity in rheumatic fever. Rev Bras Cir Cardiovasc 2014;29(1):25-30 Descriptors

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Section: Discussionsupporting

confidence: 54%

“…On a global scale, this agent is responsible for approximately 15.6 million annual cases of rheumatic heart disease, with 282,000 new cases and 233,000 deaths each year. From this perspective, health systems face higher expenses with clinical exams, surgeries and frequent hospitalizations due to congestive heart failure [ 1 - 4 ] .…”

Section: Introductionmentioning

confidence: 99%

IL-10 and ET-1 as biomarkers of rheumatic valve disease

Leão¹,

Lima²,

Nascimento³

et al. 2014

Revista Brasileira De Cirurgia Cardiovascular

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“…Mitral stenosis is an obstruction to blood inflow between left atrium and left ventricle caused by some structural abnormality of the mitral valve, such as annular calcification and chordal fusion [ 1 , 2 ] . Usually, mitral stenosis is of rheumatic origin (more than 99% of cases).…”

Section: Introductionmentioning

confidence: 99%

Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

Leão¹,

Dashwood²,

Andrade³

et al. 2015

Revista Brasileira De Cirurgia Cardiovascular

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IntroductionRheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.ObjectiveThe aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.MethodsImmunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.ResultsET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.ConclusionThe strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.

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“…Existem cepas ditas reumatogênicas, o que se deve à virulência diferenciada desse subgrupo, rico em proteína M e ácido hialurônico na sua cápsula, determinando propriedades antifagocíticas 35 . Esta condição caracteriza-se por um início febril, acompanhado de artrite grave, porém temporária e, sendo esta a característica mais comum, presente em 60%-80% dos pacientes.…”

Section: Febre Reumáticaunclassified

“…Quando submetidas à análise de sua espessura média, as valvas reumáticas revelam medidas superiores e uma quantidade acentuada de músculo e colágeno. Com o avançar da idade, há incremento de espessura, sem alteração significativa da composição valvar 35 .…”

Section: Febre Reumáticaunclassified

Análise do efeito do laser de alta potência em valvas mitrais cardíacas de suínos: estudo experimental

Pinto¹

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Amplificação dos genes que codificam a endotelina-1 e seus receptores em valvas mitrais reumáticas

Cited by 4 publications

References 9 publications

IL-10 and ET-1 as biomarkers of rheumatic valve disease

IL-10 and ET-1 as biomarkers of rheumatic valve disease

Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

Análise do efeito do laser de alta potência em valvas mitrais cardíacas de suínos: estudo experimental

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