Atividade da enzima acetil-hidrolase do fator ativador de plaquetas (PAF-AH) em pacientes com diabete melito tipo 1

Castro, Simone Henriques de; Neto, Hugo Caire de Castro Faria; Gomes, Marı́lia B.

doi:10.1590/s0066-782x2007000200008

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…However, subsequent analyses that involved multiple studies and included three of the five authors of the original article reported activity units in nmol/min/ml [49]. This unit is within the expected range of activity and is consistent with another report [57]. …”

supporting

confidence: 71%

“…Fourth, the rate of [ 3 H]acetate released from [ 3 H- acetyl ] PAF was reportedly higher than that of thiols generated from 2-thio PAF [53]. To our knowledge, only two clinical studies utilized this assay and reported somewhat lower mean plasma PAF-AH activities {(11.6 ± 4.1) [57], (15 ± 5.1) [57], and (26 ± 6) nmol/min/ml [54] 1 } compared with results from similar studies that used radiometric assays. However, differences in the composition of the patient cohorts tested in each study could account for these variations.…”

Section: Other Assays To Determine Paf-ah Activitymentioning

confidence: 99%

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Determination of phospholipase activity of PAF acetylhydrolase

Stafforini

McIntyre

2013

Free Radical Biology and Medicine

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This article presents a radiometric assay to determine the enzymatic activity of platelet-activating factor (PAF) acetylhydrolase (PAF-AH), also known as lipoprotein-associated phospholipase A2 (Lp-PLA2) and phospholipase A2 group 7A (PLA2G7A). The method is based on release of radioactively labeled acetate from sn-2-labeled PAF, and separation of substrate and product using reversed phase column chromatography on octadeyl silica gel cartridges. The assay is fast, convenient, reproducible, sensitive, and inexpensive. The instrumentation required includes standard laboratory equipment and a liquid scintillation counter. The assay is also useful to determine the activity of intracellular PAF-AH (PAF-AH II), provided that a few modifications are included. The enzymatic activity determined using PAF as the substrate is a direct indication of the ability of plasma samples, purified preparations, and cellular and tissue lysates to hydrolyze short- and medium-chain phospholipids that may or may not harbor oxidized functionalities. In addition, the assay can be used to test the suitability of other phospholipids, including species containing oxidized, long chain sn-2 fatty acyl groups, as PAF-AH substrates. This versatile assay can be used to accurately determine PAF-AH activity in biological samples and preliminarily assess affinity and efficiency of hydrolysis of potential substrates present in complex mixtures.

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supporting

confidence: 71%

Section: Other Assays To Determine Paf-ah Activitymentioning

confidence: 99%

Determination of phospholipase activity of PAF acetylhydrolase

Stafforini

McIntyre

2013

Free Radical Biology and Medicine

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“…First, Okada et al [42] showed that the Lp-PLA 2 concentration of 17 obese children (11.9 ± 0.7 years old) was positively correlated with weight, waist/height ratio, subscapular/triceps ratio and LDL-C level. Castro et al [43] compared diabetic young adults and adolescents (24.9 ± 7.8 years old) with controls (24.3 ± 9.6 years old), observing higher Lp-PLA 2 activity and elevated susceptibility for oxidized LDL in diabetic patients. Subsequently, Nagel et al [44], studying children with 10 years old, observed that overweight was positively associated with cardiometabolic markers, including Lp-PLA 2 concentration.…”

Section: Discussionmentioning

confidence: 99%

Influence of obesity and cardiometabolic makers on lipoprotein-associated phospholipase A2 (Lp-PLA2) activity in adolescents: the healthy young cross-sectional study

2013

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BackgroundLipoprotein-associated phospholipase A2 activity (Lp-PLA2) is a good marker of cardiovascular risk in adults. It is strongly associated with stroke and many others cardiovascular events. Despite this, the impact of obesity on this enzyme activity and its relation to biomarkers of cardiovascular disease in adolescents is not very well investigated. The purpose of this article is to evaluate the influence of obesity and cardiometabolic markers on Lp-PLA2 activity in adolescents.ResultsThis cross-sectional study included 242 adolescents (10–19 years) of both gender. These subjects were classified in Healthy Weight (n = 77), Overweight (n = 82) and Obese (n = 83) groups. Lipid profile, glucose, insulin, HDL size, LDL(−) and anti-LDL(−) antibodies were analyzed. The Lp-PLA2 activity was determined by a colorimetric commercial kit. Body mass index (BMI), waist circumference and body composition were monitored. Food intake was evaluated using three 24-hour diet recalls. The Lp-PLA2 activity changed in function to high BMI, waist circumference and fat mass percentage. It was also positively associated with HOMA-IR, glucose, insulin and almost all variables of lipid profile. Furthermore, it was negatively related to Apo AI (β = −0.137; P = 0.038) and strongly positively associated with Apo B (β = 0.293; P < 0.001) and with Apo B/Apo AI ratio (β = 0.343; P < 0.001). The better predictor model for enzyme activity, on multivariate analysis, included Apo B/Apo AI (β = 0.327; P < 0.001), HDL size (β = −0.326; P < 0.001), WC (β = 0.171; P = 0.006) and glucose (β = 0.119; P = 0.038). Logistic regression analysis demonstrated that changes in Apo B/Apo AI ratio were associated with a 73.5 times higher risk to elevated Lp-PLA2 activity.ConclusionsLp-PLA2 changes in function of obesity, and that it shows important associations with markers of cardiovascular risk, in particular with waist circumference, glucose, HDL size and Apo B/Apo AI ratio. These results suggest that Lp-PLA2 activity can be a cardiovascular biomarker in adolescence.

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“…Intra-assay CV of this method was 3.5 per cent and inter-assay CV was 10 per cent. The power of the study was 0.904 when calculated with true difference in patient and control means of 0.0034 and a standard deviation of 0.0034 and a standard deviation of 0.0046 taken from a previous study 20 . The Type 1 error probability associated with this test of this null hypothesis was 0.05.…”

Section: Methodsmentioning

confidence: 99%

“…The dLDL is more liable to be oxidised and can be easily taken up by macrophages in extravascular spaces resulting in atherogenesis 16 . Although some researchers have estimated the activity of this enzyme in type 1 and type 2 diabetes 17 18 19 20 , we did not come across any study specifically assessing the activity in early stages of the disease or the effect of glycemic status of the patients on this enzyme. Therefore, this study intended to evaluate the activity of Lp-PLA 2 in newly diagnosed patients of type 2 diabetes mellitus, and the correlation of this enzyme with oxLDL and plasma glucose levels was also investigated.…”

mentioning

confidence: 99%

Lipoprotein associated phospholipase A2 activity & its correlation with oxidized LDL & glycaemic status in early stages of type-2 diabetes mellitus

Garg¹,

Madhu²,

Suneja³

2015

Indian J Med Res

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Background & objectives:Lipoprotein associated phospholipase A2 (Lp-PLA2) is an important risk predictor of coronary artery disease (CAD). This study was aimed to evaluate Lp-PLA2 activity and oxidized low density lipoprotein (oxLDL) in newly diagnosed patients of type 2 diabetes mellitus and to determine the correlation of Lp-PLA2 activity with oxLDL and plasma glucose levels.Methods:Blood samples were collected in patients with newly diagnosed type 2 diabetes (n=40) before any treatment was started and healthy controls (n=40). These were processed for estimating plasma glucose: fasting and post prandial, ox LDL, and Lp-PLA2 activity. The parameters in the two groups were compared. Correlation between different parameters was calculated by Pearson correlation analysis in both groups.Results:Lp-PLA2 activity (24.48 ± 4.91 vs 18.63 ± 5.29 nmol/min/ml, P<0.001) and oxLDL levels (52.46 ± 40.19 vs 33.26 ± 12.54 μmol/l, P<0.01) were significantly higher in patients as compared to those in controls. Lp-PLA2 activity correlated positively with oxLDL in both controls (r=0.414, P<0.01), as well in patients (r=0.542, P<0.01). A positive correlation between Lp-PLA2 activity and fasting plasma glucose levels was observed only in patients (r=0.348, P<0.05).Interpretation & conclusions:Result of this study implies that higher risk of CAD in patients with diabetes may be due to increase in Lp-PLA2 activity during the early course of the disease. A positive correlation between enzyme activity and fasting plasma glucose indicates an association between hyperglycaemia and increased activity of Lp-PLA2. This may explain a higher occurrence of CAD in patients with diabetes. A positive correlation between oxLDL and Lp-PLA2 activity suggests that Lp-PLA2 activity may be affected by oxLDL also.

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Atividade da enzima acetil-hidrolase do fator ativador de plaquetas (PAF-AH) em pacientes com diabete melito tipo 1

Cited by 10 publications

References 46 publications

Determination of phospholipase activity of PAF acetylhydrolase

Determination of phospholipase activity of PAF acetylhydrolase

Influence of obesity and cardiometabolic makers on lipoprotein-associated phospholipase A2 (Lp-PLA2) activity in adolescents: the healthy young cross-sectional study

Lipoprotein associated phospholipase A2 activity & its correlation with oxidized LDL & glycaemic status in early stages of type-2 diabetes mellitus

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