Clinical and pathological assessment of 82 patients with cardiovascular diseases undergoing autopsy at the Hospital das Clínicas of the Faculdade de Medicina de Botucatu from 1988 to 1993

Oliveira, Reinaldo Ayer de; Silva, Marcos Augusto de Moraes; Andrade, Rubens Ramos de; Montenegro, M. R.

doi:10.1590/s0066-782x2000001000005

Cited by 2 publications

(2 citation statements)

References 5 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The difference in the distribution of classes of diagnostic comparison was not significantly different relative to sex, as observed previously,1 13 14 19 a different finding from a study that reported classes I and II to be more frequent in women 4…”

Section: Discussionsupporting

confidence: 62%

Comparison between clinical and autopsy diagnoses in a cardiology hospital

Saad

Yamada

Rosa

et al. 2007

Heart

View full text Add to dashboard Cite

Background: A few recent studies have evaluated diagnostic accuracy by comparison between clinical and autopsy diagnoses in a hospital specialising in cardiology. Methods: 406 consecutive autopsy cases during 2 years were studied. Patients were aged 47.4¡28.4 years; 236 (58.1%) were men and 170 (41.9%) women. Diagnostic comparison was categorised in classes I to V (I, II, III and IV: discrepancy in decreasing order of importance regarding therapy and prognosis; V: concordance). Categorisation was ranked on the basis of the highest degree of discrepancy. Statistical analysis was performed with the X 2 test and stepwise logistic regression. Results: Each age increase of 10 years added 16.2% to the risk of the diagnostic comparison to be categorised in classes I and II (major discrepancy) in comparison to classes III, IV and V (OR 1.16, 95% CI 1.07 to 1.27, p,0.001). By contrast, admission to intensive care units decreased the risk of categorisation in classes I and II by 47% (OR 0.53, 95% CI 0.32 to 0.85, p = 0.009). The most frequent diagnostic discrepancy occurred for pulmonary embolism: 30 out of 88 (34.1%) diagnoses in classes I and II. The concordance rate was 71.1% for acute myocardial infarction, 75% for aorta dissection, 73.1% for infective endocarditis and 35.2% for pulmonary embolism. Conclusion: Age and hospital ward influenced the distribution of diagnostic discrepancy or concordance between clinical and autopsy diagnoses. The lower discrepancy rate for myocardial infarction and infective endocarditis may be related to the fact that the study was carried out in a specialist hospital.

show abstract

Section: Discussionsupporting

confidence: 62%

Comparison between clinical and autopsy diagnoses in a cardiology hospital

Saad

Yamada

Rosa

et al. 2007

Heart

View full text Add to dashboard Cite

show abstract

“…However, the report by Cano et al (10) that serum ApoA1 levels are low in asymptomatic CD patients is certainly consistent with our data. The known cardioprotective effects of HDL/ApoA1 (31) and the fact that the majority of subjects who succumb to CD die from cardiac causes (17,28) lend some urgency to further studies of the interaction between T. cruzi and HDL. Whether or not the 3 biomarkers we identified in young CD subjects with EKG changes prove to be prognostically useful will be resolved by ongoing studies of samples from both CD and non-CD subjects with defined cardiac conditions.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Diagnostic Serum Biomarkers for Chagas' Disease in Asymptomatic Subjects by Mass Spectrometric Profiling

et al. 2010

View full text Add to dashboard Cite

More than 10 million people are thought to be infected with Trypanosoma cruzi, primarily in the Americas. The clinical manifestations of Chagas' disease (CD) are variable, but most subjects remain asymptomatic for decades. Only 15 to 30% eventually develop terminal complications. All current diagnostic tests have limitations. New approaches are needed for blood bank screening as well as for improved diagnosis and prognosis. Sera from subjects with asymptomatic CD (n ‫؍‬ 131) were compared to those from uninfected controls (n ‫؍‬ 164) and subjects with other parasitic diseases (n ‫؍‬ 140), using protein array mass spectrometry. To identify biomarkers associated with CD, sera were fractionated by anion-exchange chromatography and bound to two commercial ProteinChip array chemistries: WCX2 and IMAC3. Multiple candidate biomarkers were found in CD sera (3 to 75.4 kDa). Algorithms employing 3 to 5 of these biomarkers achieved up to 100% sensitivity and 98% specificity for CD. The biomarkers most useful for diagnosis were identified and validated. These included MIP1 alpha, C3a anaphylatoxin, and unusually truncated forms of fibronectin, apolipoprotein A1 (ApoA1), and C3. An antipeptide antiserum against the 28.9-kDa C terminus of the fibronectin fragment achieved good specificity (90%) for CD in a Western blot format. We identified full-length ApoA1 (28.1 kDa), the major structural and functional protein component of high-density lipoprotein (HDL), as an important negative biomarker for CD, and relatively little full-length ApoA1 was detected in CD sera. This work provides proof of principle that both platform-dependent (i.e., mass spectrometry-based) and platform-independent (i.e., Western blot) tests can be generated using high-throughput mass profiling.

show abstract

Clinical and pathological assessment of 82 patients with cardiovascular diseases undergoing autopsy at the Hospital das Clínicas of the Faculdade de Medicina de Botucatu from 1988 to 1993

Cited by 2 publications

References 5 publications

Comparison between clinical and autopsy diagnoses in a cardiology hospital

Comparison between clinical and autopsy diagnoses in a cardiology hospital

Identification of Novel Diagnostic Serum Biomarkers for Chagas' Disease in Asymptomatic Subjects by Mass Spectrometric Profiling

Contact Info

Product

Resources

About