Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay

Feferbaum, Rubens; Machado, José Kleber Kobol; Diniz, Edna Maria de Albuquerque; Okay, Thelma Suely; Santos, Sílvia Regina Cavani Jorge; Ceccon, Maria Esther Jurfest Rivero; Krebs, Vera Lúcia Jornada; Araújo, Maria Christina Barbosa de; Vaz, Flávio Adolfo Costa

doi:10.1590/s0041-87812001000500004

Cited by 6 publications

(10 citation statements)

References 10 publications

(5 reference statements)

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“…Considering that chromatography represents the method of reference for assaying medicines, and that the literature reports that the correlation coefficient among the HPLC and FPIA methods for assaying vancomycin is statistically not significant [9,10], in our laboratory we used the HPLC method because the FPIA method might introduce an overestimation of the molecule tested [11,12].…”

Section: Introductionmentioning

confidence: 99%

Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action

et al. 2008

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The Authors present a paper with a dual goal: in vitro evaluation of the elution of vancomycin and monitoring of its bactericidal action when the antibiotic is used in acrylic cement. Discs of cement with different concentrations of vancomycin alone or combined with meropenem were prepared. To assess the elution of vancomycin the discs were kept in physiological solution and periodically sampled for five weeks. The bactericidal action was assessed by putting the antibiotic discs in contact with colonies of Staphylococcus, Enterococcus, Pseudomonas and Escherichia coli. Two combinations of antibiotic-loaded cement were tested: the first one to act as a spacer and the second to stabilise the revision prosthesis.

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Section: Introductionmentioning

confidence: 99%

Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action

et al. 2008

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“…LC is an essential method for isolating and detecting vancomycin in plasma or serum. Table 3 reviews the methods and chromatographic analysis conditions for determining vancomycin in human plasma or serum [ 21 , 22 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. Undeniably, LC can analyze different body fluids such as plasma, serum, urine, cerebrospinal fluid, alveolar lavage fluid, atrial fluid, and other drug components or drug solutions prepared with different solvents.…”

Section: Lcmentioning

confidence: 99%

An Overview of Analytical Methodologies for Determination of Vancomycin in Human Plasma

Cheng

2022

Molecules

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Vancomycin is regarded as the last resort of defense for a wide range of infections due to drug resistance and toxicity. The detection of vancomycin in plasma has always aroused particular concern because the performance of the assay affects the clinical treatment outcome. This article reviews various methods for vancomycin detection in human plasma and analyzes the advantages and disadvantages of each technique. Immunoassay has been the first choice for vancomycin concentration monitoring due to its simplicity and practicality, occasionally interfered with by other substances. Chromatographic methods have mainly been used for scientific research due to operational complexity and the particular requirement of the instrument. However, the advantages of a small amount of sample needed, high sensitivity, and specificity makes chromatography irreplaceable. Other methods are less commonly used in clinical applications because of the operational feasibility, clinical application, contamination, etc. Simplicity, good performance, economy, and environmental friendliness have been points of laboratory methodological concern. Unfortunately, no one method has met all of the elements so far.

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“…12,13 For pharmacokinetics, a 1-compartment open model was applied, and the kinetic parameters were estimated using the PK Solutions ® 2.0 software (Noncompartmental Pharmacokinetics Data Analysis) Ashland, OH, USA. Statistical analysis was based on the Student t test using the GraphPad InStat ® 2.01, Graphpad Software Corporation, San Diego, USA, GMC Basic ® 6.6, Ribeirão Preto, SP, Brazil.…”

Section: Study Design Ethics and Drug Administrationmentioning

confidence: 99%

“…[11][12][13] All authors reported lower plasma levels of vancomycin (peak and trough) using HPLC compared with FPIA, indicating the higher selectivity of the HPLC method. Good correlation of laboratory data and clinical efficacy were reported for infants with sepsis by Staphylococcus species.…”

Section: Introductionmentioning

confidence: 99%

“…Good correlation of laboratory data and clinical efficacy were reported for infants with sepsis by Staphylococcus species. [11][12][13] Although pharmacokinetic data for vancomycin for pediatric patients and preterm infants are rare, a wide interindividual variation has been reported when the same dose was administered, resulting in poorly predictable plasma vancomycin concentrations. Interference of renal function with vancomycin elimination and changes in body water compartments are the main characteristics in the growing individual, and these variations might be explained according to the age of preterm infants.…”

Section: Introductionmentioning

confidence: 99%

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Vancomycin Pharmacokinetics in Preterm Infants

Machado

Feferbaum

Kobayashi

et al. 2007

Clinics

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. Vancomycin pharmacokinetics in preterm infants. CLINICS. 2007;62(4):405-10. OBJETIVE:The objective of the present study was to evaluate the kinetic disposition of vancomycin in preterm infants with emphasis on the apparent volume of distribution, biological half-life, and total body clearance as well as whether their variations cause significant modification of the trough plasma concentration of the drug, depending on the postconceptional age (PCA) and the postnatal age (PNA). MATERIAL AND METHOD: Twenty-five selected patients were distributed into 2 groups which differed significantly in terms of mean PCA (31.2-32.3 weeks in group 1, n = 13; 33.5-34.1 weeks in group 2, n = 12: CI95%, P < .001) and PNA (group 1, 12.0-18.5 days; group 2, 18.0-34.0 days, CI95%, P < .05). The parents were informed and signed a written consent for participation of the infants in the protocol that had been previously approved by the Ethics Committee of the hospital. RESULTS: Apparent volume of distribution was significantly increased in group 1 compared with patients of group 2 (0.85 vs. 0.56 L/kg, respectively; P = .01,). Additionally multiple linear regression revealed a good linear correlation (r = 0.85) of trough plasma concentration of vancomycin with the apparent volume of distribution and also with the biological half-life in patients of group 1, while a good correlation (r = 0.91) was obtained for the trough plasma concentration with total body clearance in infants of group 2. The influence of these kinetic parameters on the trough concentration of vancomycin in preterm infants seems to vary according to PCA and PNA. CONCLUSION: In conclusion, the trough plasma concentration of vancomycin depends on the pharmacokinetics, and multiple linear correlation indicates that it varies according to the postconceptional and postnatal age of preterm infants.

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Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay

Cited by 6 publications

References 10 publications

Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action

Vancomycin and meropenem in acrylic cement: elution kinetics of in vitro bactericidal action

An Overview of Analytical Methodologies for Determination of Vancomycin in Human Plasma

Vancomycin Pharmacokinetics in Preterm Infants

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