Parasitological stool sample exam by spontaneous sedimentation method using conical tubes: effectiveness, practice, and biosafety

Ribeiro, Steveen Rios; Furst, Cinthia

doi:10.1590/s0037-86822012000300024

Cited by 6 publications

(5 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fecal material was analyzed via the sedimentation method and concentration, performing three readings in each preparation [16]. In addition to basic techniques of exploratory analysis as de- …”

Section: Methodsmentioning

confidence: 99%

Enteroparasitoses in Patients with Rheumatoid Arthritis

Gomides¹,

Pires²,

Albuquerque³

et al. 2017

AID

View full text Add to dashboard Cite

Background: Patients with rheumatic diseases have profound alterations in the immune system as a result of underlying diseases and the treatments used, which increases the risk of occurrence and severity of infections, including enteroparasitosis. The current treatment for rheumatoid arthritis involves immunosuppressive therapies powerfully needed for screening infectious processes. The prevalence of parasitic infections in rheumatoid arthritis patients is not currently documented in literature. The objective of the current study was to determine the prevalence of parasitic diseases in a population with RA. Methods: We collected demographic and socioeconomic data from 67 patients at the Hospital Universitário de Brasília from July 2015 to April 2016. All patients underwent a parasitological examination of their stool and multiple variables were analyzed using Poisson regression method. Results: The mean age of patients was 53.9 years. They were predominantly in women (94%) and caucasian (47.8%). The mean disease duration was 9.2 years and most patients had the disease in remission or light activity. The prevalence of parasitic infections in these patients was 11.9%, all cases being that of protozoa of the following species: Endolimax nana, Entamoeba histolytica and Entamoeba coli. The final multivariate analysis indicated that the presence of disease had a significant statistical relationship between the presence of enteroparasitosis and rheumatoid arthritis with no fatigue by VAS (p = 0.0488) and best current health index by VAS (p = 0.0012). Conclusion: This study indicates that the prevalence of enteroparasitoses in a population with Rheumatoid Arthritis was 11.9% and all cases were found of protozoa.

show abstract

Section: Methodsmentioning

confidence: 99%

Enteroparasitoses in Patients with Rheumatoid Arthritis

Gomides¹,

Pires²,

Albuquerque³

et al. 2017

AID

View full text Add to dashboard Cite

show abstract

“…The samples thus collected were then transported in insulated boxes containing recyclable ice, to the Parasitology Laboratory of the Biomedical Institute, Fluminense Federal University. In the laboratory, the sample were processed by means of parasitological centrifugation techniques: sedimentation in conical tubes, using an adaptation of the technique described by Ribeiro & Furst (2012); and floatation in sucrose solution, as described by Sheather (1923) and modified by Huber et al (2003). The slides obtained through each technique were read and photomicrographs were produced using a binocular optical microscope (Olympus  BX 41), at a magnification of 100X.…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal parasites in stray and shelter cats in the municipality of Rio de Janeiro, Brazil

Pereira

Barbosa

Moura

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The increasingly urban nature of the population has led many people to choose independent pets, such as cats. This situation has also made it possible for these animals to be abandoned, thus increasing the numbers of cats on the streets and in shelters. These animals can act as a source of infection for other hosts. Between 2014 and 2015, the frequency of gastrointestinal parasites in captive and stray cats in the municipality of Rio de Janeiro was analyzed. Ninety-one fecal samples were collected from captive cats and 172 from stray cats. Centrifugal sedimentation and flotation techniques were used. The frequency of parasites among the stray cats was 77.3%, and this was significantly higher than the frequency observed in captive cats (49.5%). Helminths were detected more frequently, and hookworms were the parasites most detected. Toxocara cati, Cystoisospora sp. and Dipylidium caninum were also detected. No statistical difference in the frequency of parasites was observed between the sexes among the captive cats. However, among the stray cats, males (85.5%) presented higher positivity than females (71.8%). The high frequency of hookworms, which are the agent for "cutaneous larva migrans" in humans, shows the need to control parasitic infections among the cats studied.Keywords: Cats, feral cat, zoonosis, hookworms, epidemiology. ResumoO aumento da população urbana contribuiu para que muitas pessoas escolhessem um animal de estimação independente, como o gato. Esta situação possibilita o abandono desses animais, aumentando o número de gatos nas ruas e nos abrigos. Esses animais, então, podem servir como fonte de infecção para outros hospedeiros. Entre 2014 a 2015 foi analisada a frequência de parasitos gastrintestinais em gatos cativos e de rua na cidade do Rio de Janeiro. Foram coletadas 91 amostras fecais de gatos cativos e 172 de gatos de rua, sendo essas processadas pelas técnicas de centrífugo sedimentação e flutuação. A frequência de parasitos nos gatos de rua foi 77,3%, sendo esta significativamente maior do que a evidenciada nos cativos, 49,5%. Os helmintos foram mais detectados, sendo ancilostomídeos os parasitos mais evidenciados. Também foram detectados, Toxocara cati, Cystoisospora sp. e Dipylidium caninum. Não foi observada diferença estatística na frequência de parasitos entre o sexo nos gatos cativos, porém nos de rua, os machos (85,5%) apresentaram positividade maior do que as fêmeas (71,8%). A elevada frequência de ancilostomídeos, agente da "Larva Migrans Cutânea" no homem, demonstra a necessidade de controle das infecções parasitárias nos gatos estudados.Palavras-chave: Gatos, gato feral, zoonose, ancilostomídeos, epidemiologia.

show abstract

“…Among the diagnostic tests based on optical microscopy those based on spontaneous sedimentation are among the least expensive and easiest to perform and enable the simultaneous detection of helminth and protozoan intestinal infections (Brandelli et al, 2011;Camacho et al, 2013;Carvalho et al, 2012;Ribeiro and Furst, 2012;Tello et al, 2012). For these reasons, in some economically underdeveloped settings their use is preferred over the tests based on centrifuge-sedimentation or centrifuge-flotation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard

et al. 2014

View full text Add to dashboard Cite

Performance evaluation of diagnostic tests is critical in the search for accurate diagnoses. A gold standard test is usually absent in parasitology, thus rendering satisfactory assessment of diagnostic accuracy difficult. Moreover, reliability (assessed by the study of repeatability) is a rarely studied characteristic of diagnostic tests. This study compared and evaluated the performance (repeatability, concordance and accuracy) of the spontaneous sedimentation technique (SST) and the Paratest for the diagnosis of Giardia lamblia, Entamoeba histolytica complex, Blastocystis spp., Ascaris lumbricoides, hookworm, Trichuris trichiura and Calodium hepaticum. Fecal samples of 143 individuals were separated into three replicates for each test. Concordance and homogeneity of the results between replicates of each test and between tests were evaluated. Proportions of positives, sensitivity and specificity were estimated using a Bayesian Latent Class Model. High repeatability of both tests was found for the detection of intestinal parasites, except for Blastocystis spp. and hookworm. Concordance between tests was generally high (concordance correlation coefficient, 0.72-0.88), except for Blastocystis spp., hookworm and T. trichiura. The Paratest detected more cases of Blastocystis spp. and fewer of hookworm than the SST. The tests were quite discordant in the detection of T. trichiura. A low sensitivity (39.4-49.2% for SST, 35.8-53.8% for Paratest) and a high specificity (93.2-97.2%) were found for both tests. The Paratest presented a slightly higher sensitivity for the diagnosis of Blastocystis spp. (53.8%), and SST did so for hookworm (49.2%). This is the first study on repeatability and accuracy (using a Bayesian approach) of two spontaneous sedimentation techniques. These results suggest underdiagnosis of little dense parasitic forms due to technical limitations in both tests. We conclude that the combined study of repeatability, concordance and accuracy is a key strategy for better evaluation of the performance of tests and is also useful for the identification of technical limitations.

show abstract

Parasitological stool sample exam by spontaneous sedimentation method using conical tubes: effectiveness, practice, and biosafety

Cited by 6 publications

References 1 publication

Enteroparasitoses in Patients with Rheumatoid Arthritis

Enteroparasitoses in Patients with Rheumatoid Arthritis

Gastrointestinal parasites in stray and shelter cats in the municipality of Rio de Janeiro, Brazil

Comparison of the performance of two spontaneous sedimentation techniques for the diagnosis of human intestinal parasites in the absence of a gold standard

Contact Info

Product

Resources

About