Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders

Langoni, Hélio; Matteucci, Guilherme; Medici, Bruno; Camossi, Lucilene Granuzio; Richini-Pereira, Virgínia Bodelão; Silva, Rodrigo da

doi:10.1590/s0037-86822012000300016

Cited by 33 publications

(17 citation statements)

References 20 publications

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“…In addition to these confirmed reports, Langoni et al (2012) isolated N. caninum by bioassay in gerbils inoculated with brains of two of seven dogs with neurological disorders. No other details were given about these dogs or the strain of Neospora, therefore, not included in Table 1.…”

Section: Clinical Infectionssupporting

confidence: 80%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

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Section: Clinical Infectionssupporting

confidence: 80%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…There was no similarity between the genotypes found in this study and those of T. gondii isolates from Brazilian dogs in previous reports [9,26,27]. There was also no resemblance to the genotypes identified in the reports [11,13], which genotyped isolates from swine and sheep, respectively, in the same Bahia microregion as surveyed in this study (Table 2).…”

Section: Discussioncontrasting

confidence: 54%

Isolation and Genotyping of Toxoplasma gondii in Brazilian Dogs

Silva¹,

Maciel²,

Santos³

et al. 2017

Korean J Parasitol

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Strains of Toxoplasma gondii in Brazil are highly genetically diverse compared to strains from North America and Europe. Dogs are epidemiologically important because they act as sentinels for T. gondii infections in humans and are good indicators of environmental contamination. The aim of this study was to isolate and genetically characterize T. gondii strains from tissues of naturally infected Brazilian dogs. For this study, 21 blood samples were collected from dogs at the Zoonosis Control Centers of Ilhéus and Itabuna cities, Bahia, Brazil. The sera were examined for T. gondii antibodies using the indirect hemagglutination test. Brains and hearts of seropositive dogs were bioassayed in mice to isolate and characterize T. gondii parasites by PCR-RFLP using 10 genetic markers (SAG1, newSAG2, SAG3, BTUB, c22-8, c29-2, GRA6, PK1, APICO, and L358). However, T. gondii was isolated from only 4 (57.1%) dogs, designated TgDgBr6, 13, 17, and 21. All strains were virulent, causing clinical changes (rough hair coat, lethargy, and abdominal distention) and the death of all mice within 8–20 days after inoculation. Genetic analysis of these 4 T. gondii isolates revealed 4 distinct genotypes with different clonal lineage combinations (types I, II, and III) and 2 atypical alleles. Using PCR-RFLP with several markers, this study contributes to evaluations of the genetic diversity of strains circulating in Brazil.

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“…For investigation of the epidemiological status of T. gondii as well as for its detection out of clinical samples and foodstuff PCR technique had been used by various authors (Contini, Seraceni, Cultrera, Incorvaia, Sebastiani & Picot, 2005;Jauregui, Higgins, Zarlenga, Dubey, & Lunney, 2001;Langoni, Matteucci, Medici, Camossi, Richini-Pereira & Silva, 2012;Lin, Chen, Kuo, Tseng, & Tseng, 2000;Pujol-Rique, Derouin, Garcia-Quintanilla, Valls, Miro & Jimenez de Anta, 1999). The developed real time-PCR of the present study proved to be reliable for the detection of T. gondii-DNA out of different matrices.…”

Section: Discussionmentioning

confidence: 69%