Prevalence of Mansonella ozzardi among riverine communities in the municipality of Lábrea, State of Amazonas, Brazil

Medeiros, Jansen Fernandes; Py-Daniel, Victor; Barbosa, Ulysses Carvalho

doi:10.1590/s0037-86822011005000006

Cited by 19 publications

(40 citation statements)

References 16 publications

(27 reference statements)

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“…Our data support the high prevalences (>50%) of mansonelliasis in the riverine population of Purus reported in previous studies (10) (14) . However, the rate (50.3%) of M. ozzardi infections in the present study was higher than that (20.7%) in another study conducted in the Purus River region in Lábrea that used thick blood smears (4) . In other Purus River municipalities, such as Pauini and Boca do Acre, prevalences of 24.8% and 24.1%, respectively, were previously reported (3) (14) .…”

contrasting

confidence: 90%

“…The present study showed that M. ozzardi infection was present in 50.3% of the local sample, suggesting that the prevalence of this parasite in the region is higher than those recorded in recent surveys (2) (3) (4) . Furthermore, blood fi ltration using a polycarbonate membrane can be used in epidemiological surveys, especially in regions of low microfi lariae density or in post-treatment control programs, during which this method can be performed in the fi eld with minimum requirements to store blood for later analysis.…”

contrasting

confidence: 62%

“…Subsequent studies conducted in the 1970s and 1980s confi rmed the wide distribution of M. ozzardi in this region (8) (9) . More recently, the prevalence of the infection had reportedly increased in riverine communities located near the Purus and Ituxi Rivers (2) (3) (4) .…”

mentioning

confidence: 99%

“…Another important fi nding was that blood fi ltration using a polycarbonate membrane was able to detect a high number of parasitized children and adolescents aged 5-15 years (32.4%); this rate was higher than studies that used the thick blood smear method, with prevalences of 20.7%, 9.7%, and 10.2%, respectively, in children aged 10-18 years (2) (3) (4) .…”

mentioning

confidence: 99%

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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon

Basano

Medeiros

Fontes

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:Mansonella ozzardi is a widely distributed fi laria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. Methods: A diagnostic blood fi ltration method in a polycarbonate membrane was used. Results: M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fi shermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longerterm residents (≥11 years; 60.2%). Conclusions: M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.Keywords: Mansonelliasis. Mansonella ozzardi. Brazilian Amazon.Mansonella ozzardi is a widely distributed fi laria worm that parasitizes humans in Amazonas State, Brazil, with high prevalences detected in residents of some regions of the Solimões, Negro, and Purus Rivers (1) (2) (3) (4) (5) . Simuliidae (blackfl ies) are the only vector of M. ozzardi in Brazil (6) (7) .An endemic region for M. ozzardi in Brazil is in the municipality of Lábrea, in the Purus River region, Amazonas, where it has been detected since the fi rst survey in the 1950s (1) . Subsequent studies conducted in the 1970s and 1980s confi rmed the wide distribution of M. ozzardi in this region (8) (9) . More recently, the prevalence of the infection had reportedly increased in riverine communities located near the Purus and Ituxi Rivers (2) (3) (4) .In the Purus River region, only Adami et al. (10) utilized the concentration method by Knott to diagnose mansonelliasis. Therefore, the present study aimed to determine the prevalence of M. ozzardi infection in riverine communities of the Lábrea municipality using a diagnostic blood fi ltration method in a polycarbonate membrane, which is more sensitive than thick blood smears (11) .This study was conducted in the riverside communities of the Purus River (Cassianã, Bacural, Jucuri, Buraco, Santa Rosa, Jurucuá and Samaúma), located approximately 200km from the municipality of Lábrea communities (S: 07º15' 34" and W: 64º47'59"), State of Amazonas, Brazil (Figure 1). The sample consisted of volunteer men, women, and children aged 5-60 years selected using convenience sampling. Data and blood samples were collected after signing the free and informed consent form by the individual or the legal guardian. Of the estimated 350 inhabitants in the included communities, 171 (48.6%) inhabitants were present at the time of the study and agreed to participate.To estimate the prevalence of Mansonella ozzardi, blood was filtrated in a polycarbonate membrane (12) . First, 1mL venous blood was collected from each individual, diluted in 10mL 0.9% saline, and fi ltered in a polycarbonate membrane (Nucleopore Corporation, Pleasanton, CA, USA) with a 3µm pore diameter. Then, the membranes were removed from the fi lters and mounted on microscope slides, fi xed in methanol, stained with a Panoptic kit ® , and examined under an optical micros...

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confidence: 90%

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confidence: 62%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon

Basano

Medeiros

Fontes

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…Within the study area, there were no cases of onchocerciasis or bancroftiasis or any other Mansonella species. [8][9][10][11] The symptoms of mansonelliasis in humans have been extensively studied and persons with mansonelliasis have moderate fever, cold legs, arthralgia, and adenitis with dizziness and headache. 8,12,13 Currently, researchers are trying to correlate the occurrence of ocular lesions on the cornea with mansonelliasis.…”

Section: Introductionmentioning

confidence: 99%

Sustained Clearance of Mansonella ozzardi Infection after Treatment with Ivermectin in the Brazilian Amazon

Basano¹,

Fontes²,

Medeiros³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. Therapy for mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Lá brea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.

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Immune response in Mansonella ozzardi infection modulated by IL-6/IL-10 axis in Amazon region of Brazil

et al. 2018

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Mansonellosis is an endemic disease in the South and Central America. In Brazil, one of the etiological agents is Mansonella ozzardi. This filarial infection is yet poorly understood, with a controversial morbity, presenting since a oligosymptoms, malaria-like signs or without complaint in humans. The knowledge of the human immune response to microfilariae infection is limited mainly by different evolutionary cycles of the parasite in the host. In addition, the prevalence of this filarial parasite infection is high in several regions of Amazonas State. A cross-sectional study was conducted in an endemic area for microfilariae of M. ozzardi (MF) infection in the Amazonas State, Brazil. Proinflammatory and regulatory cytokines (IL-2, IL-4, IL-6, IL-10, TNF, IFN-gamma, and IL-17A) were measured in cryopreserved serum using the Cytometric Bead Array techniques (CBA) in 54 patients diagnosed with M. ozzardi infection and 55 individuals without the infection were included in the study (Controls). The IL-4, IL-6 and IL-10 level increased in infected patients with MF infection, while IL-17A increased in control only. When we compared controls to patients with high or low parasite load, the increased level of IL-6 and IL-10 were maintained. IL-6 contributes to the proinflammatory activity and IL-10 modulates Th1, Th2 and Th17 immune response. Furthermore, IL-4 was detected as a marker in the MF infection and MF patients with low parasite load, indicating the action of the Th2 cell response. The complex network of cytokines acting during M. ozzardi infection depends on a fine balance to determine a host protective effect or filarial persistence. Therefore, these results suggest that the immune response in MF infection is modulated by IL-6/IL-10 axis.

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Prevalence of Mansonella ozzardi among riverine communities in the municipality of Lábrea, State of Amazonas, Brazil

Cited by 19 publications

References 16 publications

Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon

Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon

Sustained Clearance of Mansonella ozzardi Infection after Treatment with Ivermectin in the Brazilian Amazon

Immune response in Mansonella ozzardi infection modulated by IL-6/IL-10 axis in Amazon region of Brazil

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