In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A.St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis

Abstract

INTRODUCTION: Fatty acids are abundant in vegetable oils. They are known to have antibacterial and antifungal properties. METHODS: Antifungal susceptibility was evaluated by broth microdilution assay following CLSI (formerly the NCCLS) guidelines against 16 fungal strains of clinical interest. RESULTS: In this work, fatty acid methyl esters (FAME) was able to inhibit 12 clinical strains of the pathogenic fungus Paracoccidioides brasiliensis and were also active in the bioautographic assay against Cladosporium sphaerospermum. CONCLUSIONS: FAME was a more potent antifungal than trimethoprim-sulfamethoxazole against P. brasiliensis under the experimental conditions tested.

GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF Chenopodium album AGAINST Sclerotium rolfsii

“…Chandrasekharan et al (2008) have evaluated the antimicrobial activity of four halophytes of the Chenopodiaceae family and found that fatty acid methyl esters extract of Salicornia brachiata possessed the highest antifungal activity. Lima et al (2011) have reported that fatty acid methyl esters of seeds of Annona cornifolia, mainly oleic acid methyl ester (51.5%), linoleic acid methyl ester (19.1%), and palmitic acid methyl ester (16.9%) as in the present study, have inhibited the growth of 12 strains of a clinical pathogenic fungus Paracoccidioides brasiliensis. According to Agoramoorthy et al (2007), fatty acid methyl ester extract of Excoecaria agallocha, rich in palmitic acid methyl ester (56.02%), suppressed growth of four species of Candida namely C. albicans, C .…”

The Influence of Fatty Acid Methyl Esters (FAMEs) in the Biochemistry and the Na+/K+-ATPase Activity of Culex quinquefasciatus Larvae

“…The organic phase was dissolved in hexane and then refluxed with sulfuric acid methanolic solution 2 % v/v for 60 min. Fatty acid methyl esters were obtained after extraction and solvent elimination (Lima et al 2011). The FAMEs, called 2 and 3, were produced in the Phytochemistry Laboratory of the Federal University of São João Del Rei (UFSJ) and kindly provided for use in this study.…”

“…Vegetable oils also have biological activities such as antibacterial, antifungal, and antioxidant activities, among others. The activities observed for vegetable oils are attributed mainly to the fatty acids present in the oils, being the main constituents of the oils (Chandrasekaran et al 2011;Erdemoglu and Kusmenoglu 2003;Lima et al 2011;Lima et al 2012). Further, the active fraction of the bark of Punica granatum or from the root of Canna indica inhibits the activity of acetylcholinesterase and Na ?…”

Larvicidal activity of oils, fatty acids, and methyl esters from ripe and unripe fruit of Solanum lycocarpum (Solanaceae) against the vector Culex quinquefasciatus (Diptera: Culicidae)

“…Fatty acids and methyl esters were isolated from S. lycocarpum via transesterifi cation (8) . OUF and ORF (2g each) were refl uxed with 1.0 mol/L methanolic NaOH solution for 30 min and extracted with ethyl ether.…”

“…Samples of the dried and powdered unripe (170.01g) and ripe (250.58g) fruit were subjected to oil extraction using a Soxhlet extractor with petroleum ether as the solvent (Vetec®, São Paulo, Brazil; 700mL, 6h). The extracted oils were concentrated in a rotary evaporator at 50°C under reduced pressure to produce 26.95g of oil of unripe fruit (OUF) and 29.09g of oil of ripe fruit (ORF).Fatty acids and methyl esters were isolated from S. lycocarpum via transesterifi cation (8) . OUF and ORF (2g each) were refl uxed with 1.0 mol/L methanolic NaOH solution for 30 min and extracted with ethyl ether.…”