Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Cantarelli, Vlademir Vicente; Cavalcante, Bianca; Pilger, Diogo André; Souza, Fabiana; Dias, Cícero; Brodt, Teresa; Cantarelli, Maritza; Secchi, Carina; d’Azevedo, Pedro Alves

doi:10.1590/s0037-86822011000500021

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Yurt içi ve yurt dışından yapılan çalışmalarda, bla OXA-23-like için %0-98.4 arasında pozitiflik oranları bildirilmektedir [15][16][17][18] (Tablo III) Hastane enfeksiyonlarına neden olan dirençli etkenlerin hızla tanımlanması için son yıllarda moleküler testlere sıkça başvurulmaktadır 8,9 . Ancak dirençli A.baumannii izolatlarının erken tanısı için henüz yaygın olarak kullanılan testler bulunmamaktadır.…”

Section: Discussionunclassified

“…Uygulama alanları her geçen gün genişleyen bu yöntem, antibiyotik direncinin erken belirlenmesine de imkan vermektedir. Son yıllarda hastane enfeksiyonlarına neden olan dirençli etkenlerinin erken tanısı için çok sayıda ticari kit üretilmiş ve kullanıma sunulmuştur 8,9 . Bu çalışmanın amacı, A.baumannii'nin OXA alt gruplar için multipleks qPCR kiti tasarlamak ve Türkiye'nin farklı bölgelerinden toplanan A.baumannii izolatlarında OXA alt gruplarının dağılımı araştırmaktır.…”

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Distribution of blaOXA Genes in Acinetobacter baumannii Strains: A Multicenter Study

Aşık²,

Karakeçe³

et al. 2013

Mikrobiyol Bul

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Acinetobacter baumannii is the most important agent of nosocomial infections within the Acinetobacter genus. This gram-negative coccobacillus is intrinsically resistant to many antibiotics used in antimicrobial therapy, and capable of developing resistance including carbapenems. The objective of this study was to develop a multiplex real time polymerase chain reaction (qPCR) kit for OXA subgroups in A.baumannii, and to investigate the distribution of OXA subgroups in A.baumannii strains isolated from geographically different regions of Turkey. A total of 834 A.baumannii clinical isolates collected from different state and university medical centers in 13 provinces (Afyonkarahisar, Ankara, Bolu, Elazig, Erzurum, Isparta, Istanbul, Kahramanmaras, Konya, Sakarya, Van) between 2008-2011, were included in the study. The isolates were identified by conventional methods and automated systems [Vitek2 (bioMerieux, ABD) and Phoenix (BD Diagnostic, MD)]. The susceptibility profiles of the isolates were studied with automated systems and standard disc diffusion method. All samples were subjected to qPCR to detect blaOXA-51-like, blaOXA-23-like and blaOXA-58-like genes. A conventional PCR method was also used to detect blaOXA-24-like gene. The resistance rates observed during the study period were as follows: 96.8% for amoxicillin-clavulanate, 86.8% for ciprofloxacin, 74.7% for gentamicin, 71.7% for amikacin, 73.5% for cefaperozone-sulbactam, 72.1% for imipenem and 73% for meropenem. Six hundred and two (72.2 %) isolates were resistant to both imipenem and meropenem. Colistin was found to be the most effective antibiotic against A.baumannii isolates with 100% susceptibility rate. All isolates were positive for blaOXA-51-like, however blaOXA-24-like gene could not be demonstrated in any isolate. Total positivity rates of blaOXA-23-like and blaOXA-58-like genes were found as 53.7% and 12.5%, respectively, while these rates were 74.4% and 17.3% in carbapenem-resistant isolates, respectively. Twenty-five isolates were positive for both blaOXA-23-like and blaOXA-58-like genes. All of the carbapenem-resistant isolates have OXA type genes with the exception of blaOXA-24-like gene. The positivity rates for blaOXA-23-like and blaOXA-58-like genes varied for each center. In addition, there was a decrease in the frequency of blaOXA-58-like gene, however both blaOXA-23-like gene and carbapenem resistance rates increased during the study period. In conclusion, high rates of resistance to carbapenems were also remarkable but A.baumannii strains keep on sensitivity to colistin. Both blaOXA-23-like and blaOXA-58-like genes were shown to be widespread in carbapenem-resistant A.baumannii clinical isolates. However, blaOXA-23-like gene positive strains were increased throughout the study. Currently, multiplex qPCR is the best way for rapid diagnosis of resistant bacteria for prevention of hospital-acquired infections. The multiplex qPCR kit developed in this study could be useful for rapid diagnosis and identify the frequencies of blaOXA-23-lik...

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Distribution of blaOXA Genes in Acinetobacter baumannii Strains: A Multicenter Study

Aşık²,

Karakeçe³

et al. 2013

Mikrobiyol Bul

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Antibiotic Resistance Diagnostic Methods for Pathogenic Bacteria

Marroki

Bousmaha-Marroki

2022

Encyclopedia of Infection and Immunity

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Antimicrobial resistance—Do we share more than companionship with our dogs?

Røken

Forfang

Wasteson

et al. 2022

Journal of Applied Microbiology

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Aims:To investigate and compare antimicrobial resistance genes (ARGs) in faeces from cohabiting dogs and owners. Methods and Results: DNA from faecal samples from 35 dogs and 35 owners was screened for the presence of 34 clinically relevant ARGs using high throughput qPCR. In total, 24 and 25 different ARGs were present in the dog and owner groups, respectively. The households had a mean of 9.9 ARGs present, with dogs and owners sharing on average 3.3 ARGs. ARGs were shared significantly more in households with dogs over 6 years old (3.5, interquartile range 2.75-5.0) than in households with younger dogs (2.5, interquartile range 2.0-3.0) (p = 0.02). Dogs possessed significantly more mecA and aminoglycoside resistance genes than owners.Conclusions: Dogs and owners can act as reservoirs for a broad range of ARGs belonging to several antimicrobial resistance classes. A modest proportion of the same resistance genes were present in both dogs and owners simultaneously, indicating that ARG transmission between the dog and human gut is of minor concern in the absence of antimicrobial selection. Significance and Impact of the Study:This study provides insight into the common dog and human gut resistomes, contributing to an improved knowledge base in risk assessments regarding ARG transmission between dogs and humans.

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Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Abstract: Revista da Sociedade Brasileira de Medicina Tropical 44(5):631-632, set-out, 2011Rapid detection of Van

Cited by 3 publications

References 8 publications

Distribution of blaOXA Genes in Acinetobacter baumannii Strains: A Multicenter Study

Distribution of blaOXA Genes in Acinetobacter baumannii Strains: A Multicenter Study

Antibiotic Resistance Diagnostic Methods for Pathogenic Bacteria

Antimicrobial resistance—Do we share more than companionship with our dogs?

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