Estruturas leveduriformes de Sporothrix schenckii podem gerar confusão no diagnóstico parasitológico direto da leishmaniose tegumentar americana?

Mello, Cintia Xavier de; Schubach, Armando de Oliveira; Madeira, Maria de Fátima

doi:10.1590/s0037-86822011000100031

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…The finding of S. schenckii yeast on direct examination is a rare event [22], usually occurring in cases of disseminated sporotrichosis in immunocompromised patients [5,23]. Such structures could be visualized in a case of sporotrichosis selected for this study (fig.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the finding of amastigote forms of Leishmania sp. is not always an easy task, due to the possibility of confusing them with other microorganisms or even the numerous artifacts present in smear preparations which can lead to possible misdiagnosis [5]. …”

Section: Introductionmentioning

confidence: 99%

“…Structures which present any similarity to amastigote forms, either in morphology or coloration, can lead to mistaken analysis by a less-experienced microscopist [5]. Recently, our group described a case of disseminated sporotrichosis in which a slide prepared for direct examination showed numerous rounded and ellipsoid structures located inside and outside macrophages, similar to the amastigote forms of Leishmania , alerting to a possible misdiagnosis [5]. …”

Section: Introductionmentioning

confidence: 99%

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Tegumentary Leishmaniasis: A Diagnostic Approach Considering Aspects of the Cytological Examination

Mello

Morais²,

Schubach³

et al. 2014

Acta Cytologica

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Objective: To elaborate figures that highlight the microscopic appearance of amastigote forms of the genus Leishmania and other structures subject to confusion during the routine of the direct examination of tegumentary leishmaniasis (TL). Study Design: We reviewed imprint and scraping slides previously prepared from patients with a definite diagnosis. Smear examinations were performed with an immersion objective (×1,000) selecting structures of interest for photodocumentation and elaboration of the illustrations. Results: We elaborated two 13 × 17 cm figures containing photomicrographs of amastigote forms with typical and atypical morphology of Leishmania parasites and other microorganisms, mainly fungi in the yeast-like phase and bacteria which could be confused with amastigote forms during the direct examination. Conclusions: The production of material like we show here is important and should be encouraged because of the need for constant training of professionals working in the area of TL diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tegumentary Leishmaniasis: A Diagnostic Approach Considering Aspects of the Cytological Examination

Mello

Morais²,

Schubach³

et al. 2014

Acta Cytologica

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“…The possibility of the presence of other microorganisms in the lesion, such as Sporothrix schenckii and Histoplasma capsulatum , besides artifacts, highlights the need for skilled professionals for this procedure. 3 , 4 …”

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confidence: 99%

Skin Scraping is the Most Accessible Technique for the Parasitological Diagnosis of American Tegumentary Leishmaniasis

Mello¹,

Madeira²

2015

The American Journal of Tropical Medicine and Hygiene

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Comparison of the sensitivity of imprint and scraping techniques in the diagnosis of American tegumentary leishmaniasis in a referral centre in Rio de Janeiro, Brazil

et al. 2011

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American tegumentary leishmaniasis (ATL) is an infectious disease that presents a wide spectrum of clinical manifestations making parasitological tests important for its diagnosis. Direct examination, although considered of low sensitivity is still employed mainly in areas with poor laboratory infrastructure. The aim of this study was to standardize the method of collecting and reading the scraping procedure and to then compare sensitivity of this procedure on two sites of the lesion (outer edge-OE and inner edge-IE) and of the imprint against the reference method (isolation in culture) in a group of 110 patients treated at a Referral Center in Rio de Janeiro, Brazil. ATL diagnosis was confirmed in 40 patients (36.4%), 39 cases were caused by L. braziliensis and 1 by L. amazonensis. Imprint was positive in 28 patients and scraping in OE in 17 and in IE in 25 patients, resulting in sensitivity of 70%, 42.5%, and 62.5% respectively. When the three direct examinations were combined, sensitivity value attained 77.5%. Aspects related to ease and quality of the collected material, pain intensity and frequency of bleeding in the scraping procedure were also broached and discussed in this study. The parameters of accuracy presented indicate that the direct methods can be safely used in ATL diagnosis, principally in IE scraping, as it is easy to produce and the examination is not costly, which allows the procedure to be repeated at different moments which, in turn, increases the possibility of finding the parasite. Despite that the direct methods are technically widespread, they are not standardized and the parameters of accuracy are unknown. If we consider the high incidence of leishmaniasis in low-income areas, the implantation of standardized and selective methods would provide advances in the diagnosis of leishmaniasis.

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Estruturas leveduriformes de Sporothrix schenckii podem gerar confusão no diagnóstico parasitológico direto da leishmaniose tegumentar americana?

Cited by 5 publications

References 3 publications

Tegumentary Leishmaniasis: A Diagnostic Approach Considering Aspects of the Cytological Examination

Tegumentary Leishmaniasis: A Diagnostic Approach Considering Aspects of the Cytological Examination

Skin Scraping is the Most Accessible Technique for the Parasitological Diagnosis of American Tegumentary Leishmaniasis

Comparison of the sensitivity of imprint and scraping techniques in the diagnosis of American tegumentary leishmaniasis in a referral centre in Rio de Janeiro, Brazil

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