High prevalence of methicillin-resistant Staphylococcus aureus with SCCmec type III in cystic fibrosis patients in southern, Brazil

Abstract

INTRODUCTION: Bacterial colonization of the lungs is the main cause of morbidity in cystic fibrosis (CF). Pathogens such as Staphylococcus aureus are very well adapted to the pulmonary environment and may persist for years in the same patient. Genetic determinants of these bacteria, such as the presence of SCCmec have recently emerged as a problem in this population of patients. METHODS: Staphylococcus aureus isolates obtained from different clinical materials coming from CF and non-CF patients attended at a cystic fibrosis reference hospital were compared according to SCCmec type and antibiotic susceptibility profile. RESULTS: Three hundred and sixty-four single-patient Staphylococcus aureus isolates were collected, of which 164 (45%) were from CF patients. Among the latter, 57/164 (44.5%) were MRSA, and among the non-CF patients, 89/200 (35%) were MRSA. Associated pathogens were found in 38 CF patients. All 57 MRSA from CF patients harbored the multiresistant cassette type III. In contrast, 31/89 MRSA from non-CF patients harbored SCCmec type I (35%) and 44/89 harbored type III (49%). The antibiotic susceptibility pattern was similar between CF and non-CF patients. CONCLUSIONS: The high prevalence of multiresistant SCCmec type III among CF patients compared with non-CF patients in our institution may make it difficult to control disease progression through antibiotic therapy for promoting the survival of this kind of patient.

High Frequency of icaAD, clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

“…In the study by Ghasemian et al, SCCmec type III was reported as the major SCCmec type 31 (22). In the study by Reiter, among patients with cystic fibrosis, all harbored SCCmec type III (27).…”

High biofilm production by invasive multiresistant staphylococci

“…In this study, the majority of biofilm‐producer isolates was multiresistant. Brazilian resistance rates often diverge from worldwide rates, tending to demonstrate high prevalence of multiresistant staphylococci (16, 17), may be due to frequently indiscriminate antimicrobial use in our community set. The SCCmec types IV and V isolates also demonstrated resistance to various antimicrobials other than methicillin in our study, and were found mainly in colonizing CoNS, including S. epidermidis .…”

“…Traditionally, SCCmec types I, II, and III are multiresistant hospital‐acquired cassettes, and SCCmec types IV and V carry only mecA gene and are community‐associated cassettes (12). The SCCmec structure and distribution in S. aureus and coagulase‐negative Staphylococcus (CoNS) have been studied frequently (13–16) and it could be an important factor for multiresistance development (16, 17). Besides, SCCmec is a vehicle for exchanging resistance genes between staphylococci due to ccr complex (11, 14), and biofilm formation might be a substantial advantage for this process.…”

Distribution of SCCmec types in Staphylococcus aureus Strains Isolated from the Pediatrics Suffered From Severe UTIs

“…UTIs have been described to be majorly caused by Gram-negative enterobacteria with E. coli being the most predominant (17,18). However, there is an increasing prevalence of S. aureus as a UTIs' etiologic agent in different studies including Iran (34.56%) (This study), Nigeria (33.6%) 19 , Brazil (45%) 20 and India (20.5%) 21 . Another Iranian investigation which was conducted by Momtaz and Hafezi (2014) 22 reported that of 132 clinical samples, 66 were positive for S. aureus (50%) which was entirely high.…”