Lagochilascaris minor: antibody production in experimentally infected mice

Prudente, Mariana Félix de Souza; Crespo, Adriana de Moraes Costa; Carvalhaes, Mara Sílvia

doi:10.1590/s0037-86822009000300016

Cited by 6 publications

(7 citation statements)

References 11 publications

(4 reference statements)

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“…IL-6, a multifunctional cytokine, is involved in the regulation of inflammatory responses [31]. Studies have shown that IL-6 expression is critical during the acute phase of helminth infections in mice, enhancing IgA secretion in the gastrointestinal mucosa and inducing fever during inflammation [32][33][34]. The response may play a crucial role in the immunological interaction between H. contortus and its host.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Wang,

Jin,

Kong

et al. 2024

Cells

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Background: Haemonchus contortus is a parasite widely distributed in tropical, subtropical, and warm temperate regions, causing significant economic losses in the livestock industry worldwide. However, little is known about the genetics of H. contortus resistance in livestock. In this study, we monitor the dynamic immune cell responses in diverse peripheral blood mononuclear cells (PBMCs) during H. contortus infection in goats through single-cell RNA sequencing (scRNA-Seq) analysis. Methods and Results: A total of four Boer goats, two goats with oral infection with the L3 larvae of H. contortus and two healthy goats as controls, were used in the animal test. The infection model in goats was established and validated by the fecal egg count (FEC) test and qPCR analysis of the gene expression of IL-5 and IL-6. Using scRNA-Seq, we identified seven cell types, including T cells, monocytes, natural killer cells, B cells, and dendritic cells with distinct gene expression signatures. After identifying cell subpopulations of differentially expressed genes (DEGs) in the case and control groups, we observed the upregulation of multiple inflammation-associated genes, including NFKBIA and NFKBID. Kyoto Encyclopedia of the Genome (KEGG) enrichment analysis revealed significant enrichment of NOD-like receptor pathways and Th1/Th2 cell differentiation signaling pathways in CD4 T cells DEGs. Furthermore, the analysis of ligand–receptor interaction networks showed a more active state of cellular communication in the PBMCs from the case group, and the inflammatory response associated MIF–(CD74 + CXCR4) ligand receptor complex was significantly more activated in the case group, suggesting a potential inflammatory response. Conclusions: Our study preliminarily revealed transcriptomic profiling characterizing the cell type specific mechanisms in host PBMCs at the single-cell level during H. contortus infection.

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Section: Discussionmentioning

confidence: 99%

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Wang,

Jin,

Kong

et al. 2024

Cells

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“…Lagochilascaris minor is the most important, since it is the only species causing human lagochilascariasis. 5 Animal experiments have provided insight into the life cycle of this helminth. Probably mice and other rodents are intermediate hosts for the parasite and can be infected by ingestion of eggs.…”

Section: Discussionmentioning

confidence: 99%

A Case of Lagochilascariasis in Suriname with the Involvement of the ENT System and the Skull Base

Douma

Akrum

Joe

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. We describe a case of human lagochilascariasis, with skull-base involvement and a chronic and relapsing course after treatment. This rare parasitic infection is usually manifested in the head and neck area, characterized by progressive granulomatous inflammation and the formation of abscesses. Transmission to humans most likely occurs by the consumption of undercooked meat of wild rodents. On the basis of literature studies, we propose the most likely life cycle of the parasite that involves wild feline and rodent species, with humans as accidental hosts. Even in endemic areas, it is very difficult to recognize the disease at an early stage. Progression will eventually lead to involvement of the (central) nervous system, as described in our case. Treatment is often difficult and involves resection and prolonged treatment with anthelmintic drugs. Recurrences are not uncommon and at present, long-term oral administration of ivermectin seems to be the most effective treatment.

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“…During experimental infection with Heligmosomoides polygyrus , antibodies block the intestinal invasion in the earliest days of the infection [ 34 ]. BALB/c mice have been demonstrated to produce IgM, IgG, IgA, and IgE against the parasite's crude extract (CE) and secreted-excreted (SE) antigens, with a higher production of IgA, IgM, and IgG in response to the CE antigens than to the SE products of L. minor [ 11 ]. These results suggest that the large number of CD19+ cells in the spleens of BALB/c mice is associated with the increased production of antibodies that participate in the resistance to L. minor infection.…”

Section: Discussionmentioning

confidence: 99%

“…It is evident that the difference in the susceptibility of mice to experimental lagochilascariosis can be influenced by the individual immune response, which plays an essential role in the progression of the infection by reducing or blocking the pathological processes [ 8 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…During this period, less severe pulmonary lesions and fewer subcutaneous nodules containing L3 larvae were observed in BALB/c mice than in C57BL/6 mice [ 12 ]. Moreover, we have recently demonstrated that both strains produce different classes of antibodies in response to crude extract and soluble antigens from the parasite [ 11 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Spleen Cells in Susceptible and Resistant Mice with Experimental Lagochilascariosis

et al. 2013

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Lagochilascariosis is an emerging parasitic disease caused by the helminth Lagochilascaris minor. The experimental mouse model has been used to study the immune response against L. minor infection. In the present work, immunohistochemistry analysis of spleen cells populations was evaluated in susceptible (C57BL/6) and resistant (BALB/c) mice experimentally infected with L. minor. The BALB/c mice exhibited increased spleen cell indexes as follows: F4/80+ at 100 days after infection (DPI), CD4+ at 100 and 250 DPI, CD8+ at 35 and 100 DPI, and CD19+ at 100, 150, and 250 DPI. In the spleens of the infected C57BL/6 mice, increased indexes of the following spleen cells were observed: F4/80+ cells at 250 DPI, CD4+ cells at 150 DPI, CD8+ cells at 35, 150, and 250 DPI, and CD19+ cells at 150 to 250 DPI. The index of spleen cells confirmed the differences between the control and infected groups at several time points following the infection. These data demonstrate an association between a preferential increase in the number of CD4+ and CD19+ spleen cells and resistance to experimental lagochilascariosis in BALB/c mice and between a preferential increase in the number of CD8+ spleen cells and susceptibility in C57BL/6 mice.

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Lagochilascaris minor: antibody production in experimentally infected mice

Cited by 6 publications

References 11 publications

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

A Case of Lagochilascariasis in Suriname with the Involvement of the ENT System and the Skull Base

Analysis of Spleen Cells in Susceptible and Resistant Mice with Experimental Lagochilascariosis

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