Relato de infecção natural de morcegos por flagelados tripanosomatideos em diferentes municípios do Estado do Rio de Janeiro

Barros, Juliana Helena da Silva; Romijn, Phyllis Catharina; Baptista, Cibele; Pinto, Andressa Guimarães de Souza; Madeira, Maria de Fátima

doi:10.1590/s0037-86822008000600025

Cited by 6 publications

(7 citation statements)

References 6 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies regarding differences among T. dionisii isolates are scarce, and most recent publications have focused solely on the molecular identification of this parasite in both hosts and vectors from Brazil and some other countries [ 7 , 8 , 10 , 11 , 12 , 18 ]. The three isolates of T. dionisii investigated (M1011, M1014, and M1015) were obtained in a study in different municipalities of Rio de Janeiro, where 22 samples of trypanosomatids were isolated by hemoculture of 84 bats [ 32 ]. Three samples were characterized as T. dionisii and 19 as T. madeirae [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

et al. 2020

Self Cite

View full text Add to dashboard Cite

This study describes the morphological, biochemical, and molecular differences among Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil. Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the insectivorous isolate were stumpy, reflected in statistically evident differences in the cell body and flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%), which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis. These sequences were clustered into two separate subgroups with high bootstrap values according to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii samples from bats with different feeding habits were found in the same branch. These results support the separation of the three isolates into two subgroups, demonstrating that different subpopulations of T. dionisii circulate among bats.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For blood collection, the animals were anesthetized with ketamine at a concentration calculated according to body mass, and up to 1 mL of blood was collected via cardiac puncture. Afterward, the animals were identified with a collar and released at the same capture site [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fourteen trypanosome cultures were characterized in the present study, all of which were obtained by hemoculturing from 78 specimens (21 positive for trypanosomes by hemoculture) of D. rotundus captured in the Municipal Districts of Miracema, Paraty, Maricá, Niterói, and Laje do Muriaé in the State of Rio de Janeiro (RJ) (Barros et al, 2008). Hemocultures were performed in tubes containing a biphasic medium (NNN/Schneider's) supplemented with 10% fetal calf serum (FCS), with incubation at 28 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, Neotropical bats are hosts for a large and underestimated diversity of trypanosomes that have been unraveled using molecular phylogenetic approaches. The current knowledge on the genetic diversity of trypanosomes infecting hematophagous bats suggests a great diversity of trypanosomes in D. rotundus (Barros et al, 2008; Cavazzana et al, 2010; Ramírez et al, 2014; Pinto et al, 2015; Argibay et al, 2016; Orozco et al, 2016), and Diphylla ecaudata (Cavazzana et al, 2010; Lourenço et al, 2018). In a previous study, we surveyed for trypanosomes in 78 D. rotundus captured in southeastern Brazil (Rio de Janeiro) by hemoculturing (Barros et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Barros

Lima

Schubach

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Molecular phylogenetic studies have revealed the growing diversity of bat trypanosomes. Here, 14 isolates from blood samples of the vampire bat Desmodus rotundus (Phyllostomidae) from Rio de Janeiro, Southeast Brazil, were cultivated, and morphologically and molecularly characterized. All isolates represent a novel species named Trypanosoma madeirae n. sp. positioned in the Neobat lineage of the clade T. cruzi. The Neobat lineage also comprises closely related trypanosomes of clades Neotropic 1, 2 and 3 from diverse phyllostomid species. Trypanosomes of Neotropic 1, found in Trachops cirrhosus and Artibeus jamaicensis (phyllostomids), likely represent a different species or genotype closely related to T. madeirae. Consistent with its phylogenetic positioning, T. madeirae differs from Trypanosoma cruzi in morphology of both epimastigote and trypomastigote culture forms and does not infect Triatoma infestans. Similar to its closest relatives of Neobat lineage, T. madeirae was unable to develop within mammalian cells. To date, PCR-surveys on archived blood/liver samples unveiled T. madeirae exclusively in D. rotundus from Southern to Northern Brazil. The description of a new species of bat trypanosome associated with vampire bats increases the repertoire of trypanosomes infecting D. rotundus, currently comprised of Trypanosoma cruzi, T. cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli, Trypanosoma pessoai, and Trypanosoma madeirae.

show abstract

Blood culture positivity rate for Trypanosoma cruzi in patients with chronic Chagas disease differs among different clinical forms

Nielebock

Miranda

Brasil

et al. 2020

Transactions of the Royal Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Background The purpose of this research was to compare the clinical and epidemiological characteristics of patients with chronic Chagas disease with and without positive blood cultures for Trypanosoma cruzi. Methods This was a retrospective longitudinal study that included 139 patients with chronic Chagas disease who underwent blood culture for T. cruzi. Blood cultures were performed using Novy–MacNeal–Nicolle medium enriched with Schneider's medium. Multivariate Cox proportional hazards regression analysis adjusting for age and sex was performed to identify if positive blood culture for T. cruzi was associated with all-cause mortality. Results The blood culture positivity rate was 30.9%. Most patients were born in the Northeast and Southeast regions of Brazil. Patients with positive blood cultures were older (52±13 vs 45±13 y; p=0.0009) and more frequently women (72.1% vs. 53.1%; p=0.03) than patients with negative blood cultures. The frequency of patients with cardiac or cardiodigestive forms was higher among patients with positive vs negative blood cultures (74.4% vs 54.1%; p=0.02). A total of 28 patients died during a mean follow-up time of 6.6±4.1 y. A positive blood culture was associated with all-cause mortality (hazard ratio 2.26 [95% confidence interval 1.02 to 5.01], p=0.045). Conclusions We found a higher proportion of patients with Chagas heart disease among patients with T. cruzi–positive blood cultures. A positive blood culture was associated with an increased risk of all-cause mortality. Therefore T. cruzi persistence may influence Chagas disease pathogenesis and prognosis.

show abstract

Relato de infecção natural de morcegos por flagelados tripanosomatideos em diferentes municípios do Estado do Rio de Janeiro

Cited by 6 publications

References 6 publications

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Trypanosoma madeirae sp. n.: A species of the clade T. cruzi associated with the neotropical common vampire bat Desmodus rotundus

Blood culture positivity rate for Trypanosoma cruzi in patients with chronic Chagas disease differs among different clinical forms

Contact Info

Product

Resources

About