Staphylococcus cohnii spp urealyticus: relato de caso de um patógeno incomum

d’Azevedo, Pedro Alves; Antunes, Ana Lúcia Sousa; Martino, Marinês Dalla Valle; Pignatari, Antônio Carlos Campos

doi:10.1590/s0037-86822008000200013

Cited by 4 publications

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“…S.cohnii is an opportunistic pathogen that can cause urinary tract infections, bacteremia, and sepsis ( Waldon et al., 2002 ; d’Azevedo et al., 2008 ; Soldera et al., 2013 ). Recently, S.cohnii endocarditis in the native valve was also reported in an 80-year-old patient, which indicated a poor prognosis ( Motta et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus cohnii infection diagnosed by metagenomic next generation sequencing in a patient on hemodialysis with cirrhotic ascites: a case report

Lei,

Guo,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundPatients with spontaneous bacterial peritonitis (SBP) often just receive empirical antibiotic therapy, as pathogens can be identified in only few patients using the techniques of conventional culture. Metagenomic next generation sequencing (mNGS) is a useful tool for diagnosis of infectious diseases. However, clinical application of mNGS in diagnosis of infected ascites of cirrhotic patients is rarely reported.Case presentationA 53-year-old male with cirrhosis on regular hemodialysis presented with continuous abdominal pain. After treatment with empiric antibiotics, his inflammatory parameters decreased without significant relief of abdominal pain. Finally, based on ascites mNGS detection, he was diagnosed as infection of Staphylococcus cohnii (S.cohnii), a gram-positive opportunistic pathogen. With targeted antibiotic treatment, the bacterial peritonitis was greatly improved and the patient’s abdominal pain was significantly alleviated.ConclusionsWhen conventional laboratory diagnostic methods and empirical antibiotic therapy fail, proper application of mNGS can help identify pathogens and significantly improve prognosis and patients’ symptoms.

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Section: Discussionmentioning

confidence: 99%

Staphylococcus cohnii infection diagnosed by metagenomic next generation sequencing in a patient on hemodialysis with cirrhotic ascites: a case report

Lei,

Guo,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

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Determinação da atividade antimicrobiana de duas técnicas de antissepsia cutânea utilizadas em doadores de sangue

Celere¹

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Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

Paim

Cantarelli

d’Azevedo

2014

Rev. Soc. Bras. Med. Trop.

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Introduction. The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positive human pathogens. The aim of this study was to evaluate the performance of Vitek 2 in identifying Gram-positive cocci and their antimicrobial susceptibilities. Methods. One hundred four isolates were analyzed to determine the accuracy of the automated system for identifying the bacteria and their susceptibility to oxacillin and vancomycin. Results. The system correctly identifi ed 77.9% and 97.1% of the isolates at the species and genus levels, respectively. Additionally, 81.8% of the Vitek 2 results agreed with the known antimicrobial susceptibility profi les. Conclusion. Vitek 2 correctly identifi ed the commonly isolated strains; however, the limitations of the method may lead to ambiguous fi ndings.Keywords: Gram-positive cocci. Vitek 2 automated system.Gram-positive cocci are widely distributed as part of the normal fl ora in humans; however, some species are recognized as major human pathogens and cause a large variety of infections worldwide. These microorganisms are frequently isolated from bloodstream infections, skin and soft tissue infections, sepsis, urinary tract infections and lower respiratory tract infections 1 .Automated bacterial identifi cation in the clinical laboratory provides a rapid and reliable diagnosis for most pathogens involved in infectious diseases. A previous study demonstrated the satisfactory performances of the automated methodologies, resulting in their use in routine practice with a highly acceptable level of identification accuracy; additionally, automated identification enabled the interpretation of antimicrobial susceptibility tests for the correct treatment of patients 2 .The aim of the present study was to evaluate the performance of the Vitek 2 automated system in the identifi cation of bacteria and antimicrobial susceptibilities of Gram-positive cocci isolates recovered from clinical samples and reference strains.The study was performed at Laboratório de Cocos GramPositivos (LCGP) of the Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA) and Laboratório Qualità, Rio Grande do Sul, Brazil. The isolates included in the present study were selected from the strain collection belonging LCGP. A total of 104 isolates of Gram-positive cocci were analyzed, including 29 reference strains selected from the American Type Culture Collection (ATCC) and 75 clinical strains isolated from different patients; these strains included Staphylococcus coagulase-negative (n=36), Enterococcus spp. (n=33) and Staphylococcus aureus (n=6). All of these strains have been previously characterized by the LCGP with regard to their virulence factors using molecular methods and susceptibility profi les and were identifi ed at the species level using conventional reference methods 3,4 . For the identifi cation of staphylococci, the following characteristics were tested: catalase; colony morphology and pigmentation; Gram stain; hemolysis; susceptibility to novobiocin; p...

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Staphylococcus cohnii spp urealyticus: relato de caso de um patógeno incomum

Cited by 4 publications

References 3 publications

Staphylococcus cohnii infection diagnosed by metagenomic next generation sequencing in a patient on hemodialysis with cirrhotic ascites: a case report

Staphylococcus cohnii infection diagnosed by metagenomic next generation sequencing in a patient on hemodialysis with cirrhotic ascites: a case report

Determinação da atividade antimicrobiana de duas técnicas de antissepsia cutânea utilizadas em doadores de sangue

Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

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