Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

Ribeiro-Vieira, Renata Aparecida Martinez Antunes; Ribeiro, Daniel Araki; Salvadori, Daisy Maria Fávero; Marques, Sílvio Alencar

doi:10.1590/s0037-86822007000400021

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Some studies have demonstrated that certain fungi – such as Stachybotrys chartarum, Aspergillus versicolor, Fusarium mycotoxin, Saccharomyces cerevisiae and Aspergillus flavus – also have the potential to damage DNA [ 24 - 27 ]. However, in a study of Paracoccidioides brasiliensis , this fungus was not able to induce DNA damage [ 28 ]. This finding is interesting since P. brasiliensis and L. loboi present many similarities.…”

Section: Discussionmentioning

confidence: 99%

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Barbosa

Oliveira

Tavares

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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BackgroundJorge Lobo’s disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It is caused by the fungus Lacazia loboi. Herein we report a study that relates the genotoxicity caused by L. loboi in isogenic mice with nutritional status, through a normal or restricted diet.MethodsDNA damage was assessed in the peripheral blood by the comet assay (tail intensity).ResultsThe results for leukocytes showed increases in the mean tail intensity in mice under dietary restriction, in infected mice under dietary restriction and in infected mice ingesting a normal diet.ConclusionThese results indicate that dietary restriction and L. loboi infection may increase DNA damage levels in mice, as detected by the comet assay.

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Section: Discussionmentioning

confidence: 99%

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Barbosa

Oliveira

Tavares

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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“…DNA fragmentation was analyzed by comet assay as described previously (Ribeiro-Vieira et al, 2007). After treatment, 10 µL of 16HBE cells was added to 200 µL of 0.6% low-melting-point agarose at 37°C and deposited on slides previously covered with 0.8% normal-melting-point agarose.…”

Section: Methodsmentioning

confidence: 99%

“…The fluorescence staining experiments were conducted as described previously (Ribeiro-Vieira et al, 2007). After treatment, cells grown on coverslips were fixed in freshly prepared 4% paraformaldehyde in PBS for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Effects of chromium (VI) on the toxicity of benzo[z]pyrene in 16HBE cells

Wang

Xia

2022

Toxicol Ind Health

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Contamination of human habitats with complex mixtures of heavy metals and polycyclic aromatic hydrocarbons (PAHs) is an important environmental and industrial health problem. Hexavalent chromium (Cr(VI)) and benzo(a)pyrene (B[a] P) are typical of the two, respectively. In recent decades, a great deal of research has focused on their carcinogenicity and mechanisms of action. However, few studies have been conducted to evaluate their combined effects on humans and cells, which has important implications for overall understanding of their toxicity and interaction. In the current study, the combined toxic effects of B[a] P and Cr(VI) were studied in human bronchial epithelial cells (16 HBE). We measured the genotoxic activity and epigenetic changes of these two toxicants alone and in combination on these cells and analyzed the difference between their single and combined toxicity. The results showed that B[a]P caused DNA damage in 16HBE cells in a concentration-dependent manner, while the presence of Cr(VI) showed a sharp decrease in DNA damage, and it inhibited the expression of genes related to base excision repair induced by B[a]P. In addition, Cr(VI) also reduced B[a]P-triggered epigenetic changes in 16HBE cells. In conclusion, the combined effect of B[a]P and Cr(VI) on 16HBE cells was less toxic than single B[a]P exposure, indicating that the combined toxicity of the two toxicants is partially antagonistic. Further research is required to explore the mechanism of this antagonism.

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Short-term and long-term exposure to hexavalent chromium alters 53BP1 via H3K18ac and H3K27ac

et al. 2019

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Paracoccidioidomycosis: no genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

Abstract: Paracoccidioidomycosis is a systemic fungal infection caused by

Cited by 3 publications

References 12 publications

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Effects of chromium (VI) on the toxicity of benzo[z]pyrene in 16HBE cells

Short-term and long-term exposure to hexavalent chromium alters 53BP1 via H3K18ac and H3K27ac

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