Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Pereira, Cláudio Márcio do Nascimento Abreu; Possas, Cristina; Viana, Célio Mauro; Rodrigues, Dália dos Prazeres

doi:10.1590/s0037-86822007000100011

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…In our work, and that of Chen et al (2005), collections were made during cold months, which might have influenced the number of bacterial isolates obtained. Our results were lower in comparison to the studies of Vieira et al (2004) and Pereira et al (2007), which were conducted in climates with warmer temperatures. V. parahaemolyticus is most frequently found in estuaries, sediments, mollusks, and crustaceans (ICMSF, 1978).…”

Section: Discussioncontrasting

confidence: 99%

“…obtained in SP where the climate is considered subtropical, as well as in the region where our work was performed, in which the microorganism was isolated from 4.61% (6 of 130) of the analyzed samples. Pereira et al (2007) isolated V. parahaemolyticus from 11.6% of 86 mussel (Perna perna) samples from the town of Niterói, Rio de Janeiro (RJ), which has a tropical climate. In Fortaleza, Ceará, where the climate is semiarid, Vieira et al (2004)analyzed90 samples of freshwater crabs (Ucides cordatus), of which 8.9% tested positive for V. parahaemolyticus.…”

Section: Discussionmentioning

confidence: 99%

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Vibrio parahaemolyticus e Salmonella enterica isolados de pescados capturados no estuário da Lagoa dos Patos

Rosa

Silva

Barbosa

et al. 2016

SCA

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Microorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were eviscerated and cleaned for trade. Thirteen fish landing were analyzed and 65 entire fishes and 65 cleaned fishes were studied to determine the presence of V. parahaemolyticus and S. enterica. Bacterial isolates were compared using rep-PCR. V. parahaemolyticus was isolated from one entire Micropogonias furnieri and two entire Mugil platanus, as well as from three eviscerated M. platanus. S. enterica was isolated from two eviscerated Paralichthys orbignyanus. Identical rep-PCR bands from V. parahaemolyticus were observed in entire and eviscerated fishes from the same discharge, suggesting processing failures that neither eliminated the microorganism from the raw material nor prevented cross-contamination. S. enterica was not isolated from entire fishes, presumably because contamination occurred due to hygiene and sanitary failures. Our results showed that M. furnieri and M. platanus captured in the Lagoa dos Patos estuary may host V. parahaemolyticus and that this microorganism, as with S. enterica, may also persist even after the fish is cleaned. This is the first record of the isolation of V. parahaemolyticus from M. furnieri.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vibrio parahaemolyticus e Salmonella enterica isolados de pescados capturados no estuário da Lagoa dos Patos

Rosa

Silva

Barbosa

et al. 2016

SCA

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“…Moreover, V. parahaemolyticus infections have increased globally 28,33 . Studies in Brazil demonstrated the presence of V. parahaemolyticus in environmental samples from a variety of sources as well as in clinical samples 1,25,26,27,31,34,35,38 . In 2002, according to data of the National Health Foundation (FUNASA), in Ceará, Brazil, an outbreak of gastroenteritis occurred, and Kanagawa-positive strains of V. parahaemolyticus serovar O3:K6 were isolated 31 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Rojas

Matté

Dropa

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYVibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the bla TEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.

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“…In this context, Hc030 and Hc3060 protein fractions inhibited the growth of V. parahaemolyticus, an important gram-negative pathogen that causes human gastroenteritis associated with seafood consumption without appropriate cooking (Pereira et al 2007, Su & Liu 2007. So, plant-derived proteins would be a good source of antibacterial compounds.…”

Section: Discussionmentioning

confidence: 99%

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

Brito

Melo

Alves

et al. 2016

Hoehnea

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-(Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions). The crude extract and protein fractions of Hymenaea courbaril L. seeds were investigated for the presence of trypsin and papain inhibitors and antimicrobial activity against Vibrio parahaemolyticus, Staphylococcus aureus, and Escherichia coli. Protein fractions were obtained from the crude extract after precipitation with ammonium sulfate into three saturation ranges (0-30%, 30-60%, and 60-90%), called Hc030, Hc3060, and Hc6090, respectively. The crude extract and protein fractions inhibited trypsin and papain activity, but to different degrees. Antimicrobial activity was observed in Hc030 and Hc3060 fractions, but only against V. parahaemolyticus.The inhibitor isolated from the Hc3060 fraction was more effective in inhibiting trypsin (100% inhibition) than papain (54% inhibition), and showed an apparent molecular mass of 20 kDa. This study shows that H. courbaril seeds contain proteins with protease-inhibiting and antibacterial activity, indicating that this species is a source of bioactive compounds. Keywords: antimicrobial, cystatin, Fabaceae, protease RESUMO -(Purificação parcial de um inibidor de tripsina/papaína de sementes de Hymenaea courbaril L. e atividade antibacteriana de suas frações proteicas). O extrato bruto e frações proteicas de sementes de Hymenaea courbaril foram investigados para a presença de inibidores de tripsina e papaína e atividade antimicrobiana contra Vibrio parahaemolyticus, Staphylococcus aureus e Escherichia coli. As frações foram obtidas após precipitação do extrato bruto com sulfato de amônio em três faixas de saturação (0-30%, 30-60% e 60-90%) e denominadas Hc030, Hc3060 e Hc6090, respectivamente. O extrato bruto e as frações apresentaram diferente especificidade em inibir a atividade da tripsina e papaína. A atividade antimicrobiana foi observada nas frações Hc030 e Hc3060 e somente contra V. parahaemolyticus. O inibidor isolado a partir de Hc3060 foi mais eficiente na inibição da tripsina (100% de inibição) do que da papaína (54% de inibição), e mostrou uma massa molecular aparente de 20 kDa. Este estudo mostra que as sementes de H. courbaril possuem inibidores de proteases e proteínas com atividade antibacteriana, indicando que a espécie é uma fonte de compostos bioativos.

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Características de Vibrio parahaemolyticus isolados de mexilhões (Perna perna) comercializados em Niterói, Rio de Janeiro

Cited by 15 publications

References 11 publications

Vibrio parahaemolyticus e Salmonella enterica isolados de pescados capturados no estuário da Lagoa dos Patos

Vibrio parahaemolyticus e Salmonella enterica isolados de pescados capturados no estuário da Lagoa dos Patos

Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil

Partial purification of trypsin/papain inhibitors from Hymenaea courbaril L. seeds and antibacterial effect of protein fractions

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