Estudo das fontes alimentares de Panstrongylus lutzi (Neiva &amp; Pinto, 1923) (Hemiptera: Reduviidae: Triatominae) no Estado do Ceará

Caranha, Lindembergh; Lorosa, Elias Seixas; Rocha, Dayse da Silva; Jurberg, José; Galvão, Cléber

doi:10.1590/s0037-86822006000400006

Cited by 17 publications

(5 citation statements)

References 15 publications

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“…This species was the fifth most frequently found in captures across 12 Brazilian states in recent years [13, 105]. P. lutzi occurs in Caatinga and can be found inhabiting armadillo nests [113]; however, it has a much more eclectic diet in domestic environments and shows high levels of natural infection [45]. …”

Section: Discussionmentioning

confidence: 99%

Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling

Gurgel-Gonçalves

Galvão

Costa

et al. 2012

Journal of Tropical Medicine

191

182

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Although Brazil was declared free from Chagas disease transmission by the domestic vector Triatoma infestans, human acute cases are still being registered based on transmission by native triatomine species. For a better understanding of transmission risk, the geographic distribution of Brazilian triatomines was analyzed. Sixteen out of 62 Brazilian species that both occur in >20 municipalities and present synanthropic tendencies were modeled based on their ecological niches. Panstrongylus geniculatus and P. megistus showed broad ecological ranges, but most of the species sort out by the biome in which they are distributed: Rhodnius pictipes and R. robustus in the Amazon; R. neglectus, Triatoma sordida, and T. costalimai in the Cerrado; R. nasutus, P. lutzi, T. brasiliensis, T. pseudomaculata, T. melanocephala, and T. petrocchiae in the Caatinga; T. rubrovaria in the southern pampas; T. tibiamaculata and T. vitticeps in the Atlantic Forest. Although most occurrences were recorded in open areas (Cerrado and Caatinga), our results show that all environmental conditions in the country are favorable to one or more of the species analyzed, such that almost nowhere is Chagas transmission risk negligible.

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Section: Discussionmentioning

confidence: 99%

Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling

Gurgel-Gonçalves

Galvão

Costa

et al. 2012

Journal of Tropical Medicine

191

182

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“…The species P. lutzi and T. sordida , which have both been previously reported in the state of Piauí 9 , were also collected in the study area. Panstrongylus lutzi is frequently found in the northeastern region of Brazil 18 , 19 , 22 and presents greater importance as a T. cruzi vector because it can be found in the intradomicile and present high levels of natural infections 22 , 23 . In contrast, T. sordida is typical of the cerrado biome (central region of Brazil) and occurs in the northeast region mainly in areas bordering that biome with the caatinga 14 .…”

Section: Discussionmentioning

confidence: 99%

Triatomine bugs (Hemiptera, Reduviidae, Triatominae) in the Domiciles of the Guaribas Valley Territory, in Northeastern Brazil

Mendes-Sousa

Araújo

Sousa

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Triatomine bugs are hematophagous insects that are extremely important in public health because they are natural vectors of Trypanosoma cruzi, the causative agent of Chagas disease. In this study, we aimed to assess the occurrence of triatomine species and the natural T. cruzi infection in the Guaribas Valley territory, an endemic region for Chagas disease in northeastern Brazil. Methods: Insects were actively captured from July 2017 to October 2019 in the intra-and peridomiciles of 16 municipalities of the Guaribas Valley territory, in the southeast area of Piauí state. Triatomine species were identified following a taxonomic key, and natural infection was investigated through insects' fresh feces exams. Results: A total of 430 triatomines were collected, including 211 nymphs and 219 adults. Of all collected specimens, 39 (9.1%) were from the intradomiciles and 391 (90.9%) from peridomiciles. Nine species, including two subspecies, could be identified: Triatoma brasiliensis brasiliensis, T. brasiliensis macromelasoma, T. pseudomaculata, T. sordida, T. juazeirensis, T. melanocephala, Panstrongylus lutzi, Rhodnius domesticus, R. nasutus, and R. robustus. T. brasiliensis were the most frequently collected bugs, representing 72% of all the identified insects. None of the examined invertebrates presented flagellate forms of T. cruzi. Conclusions: This is the first report of T. b. macromelasoma and T. juazeirensis in the Guaribas Valley territory. The persistence of triatomine species in the domiciles in an endemic area for Chagas disease emphasizes the relevance of entomological surveillance and vector control measures in the studied area.

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“…Panstrongylus lutzi is one of the native species of northeastern Brazil, and its geographical distribution coincides with the extension of the Caatinga biome. Mainly in Ceará and Pernambuco states, it is increasingly common the presence of adults in the intradomicile and generally presenting high rates of T. cruzi infection, elevating the epidemiological importance of this species [44]. Studies evaluating natural infections by T. cruzi in P. lutzi have been reported using parasitological search by microscopy; in one of these, T. cruzi flagellates were identified in 29.1% of the specimens found in the intradomicile, in 20 municipalities of Ceará [44].…”

Section: Discussionmentioning

confidence: 99%

“…Mainly in Ceará and Pernambuco states, it is increasingly common the presence of adults in the intradomicile and generally presenting high rates of T. cruzi infection, elevating the epidemiological importance of this species [44]. Studies evaluating natural infections by T. cruzi in P. lutzi have been reported using parasitological search by microscopy; in one of these, T. cruzi flagellates were identified in 29.1% of the specimens found in the intradomicile, in 20 municipalities of Ceará [44]. As mentioned, the high positivity found in the present study for kDNA-PCR may have been overestimated due to the collection of a small number of P. lutzi specimens and the vast majority being infected.…”

Section: Discussionmentioning

confidence: 99%

Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

et al. 2017

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BackgroundChagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. The diversity of vector species and their habitats are among the factors that hinder the control of the disease. Control programs periodically monitor the prevalence of T. cruzi infection in insect bugs through microscopical observation of diluted feces. However, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all evolutionary stages of the insect and may in turn be limited to differentiate T. cruzi from other morphologically similar trypanosomatids. Here, we report two highly sensitive and accurate methodologies to infer T. cruzi infection rates and to quantify parasite load in the gut of field-collected triatomines.MethodsTriatomines were manually collected in the period 2011–2012 and 2014–2015, in domestic, peridomestic or sylvatic habitats in rural areas of 26 municipalities, encompassing three distinct Brazilian biomes: Caatinga, Cerrado and Atlantic Rainforest. Following morphological and taxonomical identification, the search for flagellated protozoa was performed by optical microscopy. A conventional PCR targeting T. cruzi kDNA and a TaqMan qPCR directed to the parasite nuclear satellite DNA (SAT) were developed, both in multiplex, with the triatomine 12S subunit ribosomal RNA gene, used as internal amplification control. Both methods were used for detection (kDNA-PCR) and parasite load quantification (SAT-DNA-qPCR), to investigate T. cruzi infection in captured triatomines.ResultsThe combined methods were assayed on a panel of 205 field-collected triatomine samples. Diagnostic analysis revealed 21% positivity for the kDNA-PCR, whereas microscopic examination enabled identification of T. cruzi in only 7.0% of the PCR-positive samples. Negative PCR results were confirmed by the absence of T. cruzi flagellates using microscopy. Caatinga biome yielded the highest T. cruzi infection rate (60%), followed by the Atlantic Rainforest and Cerrado with 7.1 and 6.1%, respectively. In addition, a wide range distribution of parasite load, varying from 8.05 × 10-2 to 6.31 × 1010 was observed with a median of 2.29 × 103 T. cruzi/intestine units. When parasite load was analyzed by triatomine species, a significantly higher median was found for Panstrongylus lutzi in comparison with Triatoma brasiliensis.ConclusionsOur results demonstrate highly sensitive PCR-based methodologies to monitor T. cruzi infection in triatomines. In addition, the qPCR assay offers the possibility of further evaluation parasite load, as a promising biomarker of the vectorial capacity of triatomines in Chagas disease endemic areas.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2343-x) contains supplementary material, which is available to authorized users.

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Estudo das fontes alimentares de Panstrongylus lutzi (Neiva & Pinto, 1923) (Hemiptera: Reduviidae: Triatominae) no Estado do Ceará

Cited by 17 publications

References 15 publications

Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling

Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling

Triatomine bugs (Hemiptera, Reduviidae, Triatominae) in the Domiciles of the Guaribas Valley Territory, in Northeastern Brazil

Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

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