Prevalência de genótipos e de mutantes pré-core A-1896 do vírus da hepatite B e suas implicações na hepatite crônica, em uma população da Amazônia oriental

Conde, Simone Regina Souza da Silva; Moia, Lizomar de Jesus Maués Pereira; Barbosa, Marco Aurélio Alves de Freitas; Amaral, Ivanete do Socorro Abraçado; Miranda, Esther Castello Branco Mello; Soares, Manoel do Carmo Pereira; Brito, Elizabete Maria de Figueiredo; Souza, Olglaíze do Socorro Costa; Araújo, Marialva Tereza Ferreira de; Demachki, Sâmia; Rebello, João Renato Pinho; Mesquita, Michele Gomes Soares; Bertollini, Denis Alberto; Ishak, Ricardo

doi:10.1590/s0037-86822004000700005

Cited by 15 publications

(12 citation statements)

References 37 publications

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“…Sitnik et al, studying the prevalence of HBV genotypes in chronic carriers from different regions of Brazil, also found genotype C in individuals of Asian origin 5,17 . Our results agree with other Brazilian studies [18][19][20][21][22] showing that genotypes A and D were the most frequent in the general population. It is interesting to observe that viral load was higher in genotype D carriers than in the other carriers.…”

Section: Discussionsupporting

confidence: 93%

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Hepatitis B virus infection in children, adolescents, and their relatives: genotype distribution and precore and core gene mutations

Compri

Miura

Porta

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION:The objectives of this study were evaluate hepatitis B virus (HBV) serological markers in children and adolescents followed up at the Child Institute of the Hospital das Clínicas, Faculdade de Medicina de São Paulo, Universidade de São Paulo; identify chronic HBV carriers and susceptible individuals in the intrafamilial environment; characterize HBV genotypes; and identify mutations in the patients and household contacts. METHODS: Ninety-five hepatitis B surface antigen-positive children aged <19 years and 118 household contacts were enrolled in this study. Commercial kits were used for the detection of serological markers, and PCR was used for genotyping. RESULTS: Hepatitis B e antigen (HBeAg) was detected in 66.3% (63/95) of cases. Three of the 30 HBeAg-negative and anti-HBeAg-positive patients presented with precore mutations and 11 presented with mutations in the basal core promoter (BCP). Genotype A was identified in 39 (43.8%) patients, genotype D in 45 (50.6%), and genotype C in 5 (5.6%). Of the 118 relatives, 40 were chronic HBV carriers, 52 presented with the anti-HBc marker, 19 were vaccinated, and 7 were susceptible. Among the relatives, genotypes A, D, and C were the most frequent. One parent presented with a precore mutation and 4 presented with BCP mutations. CONCLUSIONS: Genotypes A and D were the most frequent among children, adolescents, and their relatives. The high prevalence of HBV in the families showed the possibility of its intrafamilial transmission.

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Section: Discussionsupporting

confidence: 93%

“…FINANCIAL SUPPORT REFERENCES investigators 5,19,23 . Mutations are more frequent in the BCP region than in the precore and core regions.…”

Section: Conflict Of Interestmentioning

confidence: 99%

Hepatitis B virus infection in children, adolescents, and their relatives: genotype distribution and precore and core gene mutations

Compri

Miura

Porta

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…Studies on the distribution of HBV genotypes in Brazil and the Amazon also show that in the northern region, genotype A is the most common, followed by genotype D and genotype F 12,[17][18][19] . However, other studies performed in the Brazilian Amazon revealed that genotype F is more prevalent [20][21][22] .…”

Section: Ethical Considerationsmentioning

confidence: 74%

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Dias

Oliveira

Castilho

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Hepatitis B virus (HBV) infection is a serious public health issue worldwide. Hepatitis B virus is classified into eight genotypes, varying from A to H, with distinct geographical distributions. In Brazil, the most frequent genotypes are A, D, and F. METHODS: This study aimed to characterize the HBV genotypes in cases of hepatitis B virus and hepatitis D virus (HDV) co-infections in an endemic area in the Western Brazilian Amazon. We analyzed 86 serum samples reactive for HBsAg from indigenous and non-indigenous populations obtained from previous serological surveys. RESULTS: Of the 86 reactive serum samples, 39 were found to be HBV-DNA-positive by semi-nested PCR. The genotypes were established by sequencing the amplified S gene region. We obtained 20 sequences classified into three genotypes: A, D, and F. Genotype A was the most frequent (60%), followed by D (35%) and F (5%). CONCLUSIONS: The distribution of the HBV genotypes reflected the pattern of historical occupation of the region.

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“…Other authors [35] corroborate the present study with regard to the predominance of genotype A in Brazilian regions; however, in other studies, genotype F has greater frequency among isolated tribes of Amazonia who do not maintain contact with whites. In this same study, it was shown that contact with whites modified the prevalence of the genotypes favoring the predominance of genotype A [36,37]. These data suggest a change in the native genotypic profile of the region, possibly influenced by the migratory dynamics that has been occurring in western Amazonia since the end of the 19 th century and beginning of the 20 th century, characterized in this study by the increased prevalence of genotype A in relation to genotype F, seen as the native genotype of this region.…”

Section: Clinico-epidemiological Manifestationsmentioning

confidence: 80%

Characterization of HBeAg-negative chronic hepatitis B in western Brazilian Amazonia

Victoria¹,

Oliveira²,

Victoria³

et al. 2008

Braz J Infect Dis

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The present study was conducted with 55 patients native from western Brazilian Amazonia, who were HBV-DNA positive after seroconversion of HBeAg. It is a descriptive case study, with the patients separated into two groups: with hepatitis and without hepatitis on histological examination. The aim of the present study was to describe the clinical and molecular characteristics of patients who are chronic carriers of HBsAg. The prevalence of hepatitis was 63.64%, with a predominance of males (41.82%) and a mean age of 42.5 years, occurring mostly in natives of the southeast sub-region (32.73%). Time was a variable proportional to the course of the disease and the most frequent symptoms were: dyspepsia, asthenia and loss of libido with the majority of the patients having history of prior contact with HBV or positive family history. Splenomegalia was the most frequent sign (40%). Among the tests, platelet count, serum albumin and prothrombin activity were significant in the diagnosis of hepatitis. Alphafetoprotein was greater in patients with hepatitis, and hepatocellular carcinoma was detected in 3.63% of the patients with hepatic cirrhosis. Three types of HBV genotypes were diagnosed: A, D and F in the samples amplified for gene S. Genotype A (AA) was observed in 54.54% of the cases with hepatitis, in contrast to other studies showing the predominance of genotype F in this region. We observed mutations in 36.36%, with a predominance of the mutations in the core promoter region (31.81%), due to the greater prevalence of genotype A in this study. Key-Words: HBeAg-negative chronic hepatitis B, western Brazilian Amazonia, mutations.Western Brazilian Amazonia is considered a region of high endemicity for hepatitis B virus (HBV), where a large part of the population is infected in perinatal period and during infancy. The prevalence of chronic carriers of HBV surface antigen (HBsAg) in these areas varies between 5% and 15% and approximately 50% to 95% of the population show serologic evidence of past infection with HBV [1,2,3]. Various factors are implicated in the development and outcome of HBV infection, but the progression to chronic hepatitis is proportionally greater in individuals infected by the vertical route or during the perinatal period [4]. Chronic hepatopathies related to HBV are seen in two forms: HBeAg positive and HBeAg negative. The HBeAg-negative chronic forms are represented by two groups of patients: chronic inactive HBsAg carriers and chronic hepatitis B (CHB) patients. The distinction between these two forms is difficult, particularly in patients with biochemical and intermittent virologic activity. It is estimated that in the world there are approximately 350 million inactive HBsAg carriers, and of these, 7% to 30% are infected with mutant forms of HBV, which despite the presence of anti-HBe antibody continue to be HBV-DNA positive [5]. Chronic HBsAg carriers who show HBeAg-negative/antiHBe-positive serologic status and have serum HBV-DNA detectable by PCR and active hepatic disease characterized by ...

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Prevalência de genótipos e de mutantes pré-core A-1896 do vírus da hepatite B e suas implicações na hepatite crônica, em uma população da Amazônia oriental

Cited by 15 publications

References 37 publications

Hepatitis B virus infection in children, adolescents, and their relatives: genotype distribution and precore and core gene mutations

Hepatitis B virus infection in children, adolescents, and their relatives: genotype distribution and precore and core gene mutations

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Characterization of HBeAg-negative chronic hepatitis B in western Brazilian Amazonia

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