Padronização da técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina para a identificação de sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz &amp; Neiva, 1912)

Marassá, Ana Maria; Consales, Cleide Aschenbrenner; Galati, Eunice Aparecida Bianchi

doi:10.1590/s0037-86822004000600003

Cited by 15 publications

(13 citation statements)

References 20 publications

(11 reference statements)

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“…Para os controles positivo e negativo do teste, foram utilizados 60 exemplares de L. longipalpis, conforme descrito na padronização da técnica imunoenzimática de captura para a pesquisa do hábito alimentar em flebotomíneos 7 . Preparo das amostras.…”

Section: Methodsunclassified

Identificação do sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912) e Lutzomyia (Lutzomyia) almerioi (Galati & Nunes, 1999) pela técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina

Marassá

Consales

Galati

et al. 2006

Rev. Soc. Bras. Med. Trop.

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RESUMOLutzomyia longipalpis e Lutzomyia almerioi, espécies integrantes da fauna flebotomínea da Serra da Bodoquena, no Estado de Mato Grosso do Sul, têm sido objeto de estudo devido às suas elevadas abundâncias no Assentamento Guaicurus, foco de leishmaniose tegumentar humana e visceral canina. Em pesquisas que vem sendo realizadas neste acampamento para a identificação de vetores destas parasitoses, foram capturados no período de 2002 a 2004, com armadilhas automáticas luminosas, instaladas em ambiente peridoméstico (galinheiro), 83 exemplares ingurgitados de Lutzomyia longipalpis e Lutzomyia almerioi. O presente estudo teve como objetivo a investigação do hábito alimentar para ave das fêmeas de ambas as espécies de flebotomíneos, mediante o emprego da técnica imunoenzimática de captura,comparando-se a reatividade durante os anos de 2002 a 2004. Dentre 57 amostras de Lutzomyia longipalpis e 26 de Lutzomyia almerioi, foram encontradas 72% reagentes para ave em Lutzomyia longipalpis e 96% em Lutzomyia almerioi, o que justifica o estudo do hábito alimentar na região, como medida de prevenção e instituição de vigilância epidemiológica. Palavras-chaves: Identificação de sangue ingerido. Lutzomyia longipalpis. Lutzomyia almerioi. Técnica imunoenzimática de captura. Vetores. Abstract Lutzomyia longipalpis and Lutzomyia almerioi, phlebotomine species from the fauna of Serra da Bodoquena, in the State of Mato Grosso do Sul, Brazil, have been studied, particularly due to the fact of their abundance and occurrence, the Guaicurus settlement, focus of human tegumentary and canine visceral leishmaniasis. In researches that are being carried out in this settlement for identifying the vectors of these parasitosis, 83 engorged females belonging to the species Lutzomyia longipalpis and Lutzomyia almerioi were captured with automatic light traps from 2002 up to 2004 in the peridomiciliary environment of the Guaicurus settlement (hennery).The aim of this study was the investigation on bird feeding habit of females of both the phlebotomine species by the enzyme-linked immunosorbent assay technique, comparing the reactivity during the period from 2002 up to 2004. Of the 57 samples of Lutzomyia longipalpis and 26 of Lutzomyia almerioi that have been tested, 72% from Lutzomyia longipalpis and 96% from Lutzomyia almerioi were reactive, which justifies the feeding habit study in the region as a prevention measure and the institution of an epidemiological survey.

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Section: Methodsunclassified

Identificação do sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912) e Lutzomyia (Lutzomyia) almerioi (Galati & Nunes, 1999) pela técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina

Marassá

Consales

Galati

et al. 2006

Rev. Soc. Bras. Med. Trop.

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“…Results were expressed as absorbance and, for higher specificity, the cut-off value was set by adding up three standard deviations to the arithmetic mean of serum absorbances 7,11,16 .…”

Section: Cut-off Settingmentioning

confidence: 99%

Circulation of antibodies against yellow fever virus in a simian population in the area of Porto Primavera Hydroelectric Plant, São Paulo, Brazil

Lima

Romano-Lieber

Duarte

2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYYellow fever (YF) is an acute viral infectious disease transmitted by mosquitoes which occurs in two distinct epidemiological cycles: sylvatic and urban. In the sylvatic cycle, the virus is maintained by monkey's infection and transovarian transmission in vectors. Surveillance of non-human primates is required for the detection of viral circulation during epizootics, and for the identification of unaffected or transition areas. An ELISA (enzyme-linked immunosorbent assay) was standardized for estimation of the prevalence of IgG antibodies against yellow fever virus in monkey sera (Alouatta caraya) from the reservoir area of Porto Primavera Hydroelectric Plant, in the state of São Paulo, Brazil. A total of 570 monkey sera samples were tested and none was reactive to antibodies against yellow fever virus. The results corroborate the epidemiology of yellow fever in the area. Even though it is considered a transition area, there were no reports to date of epizootics or yellow fever outbreaks in humans. Also, entomological investigations did not detect the presence of vectors of this arbovirus infection. ELISA proved to be fast, sensitive, an adequate assay, and an instrument for active search in the epidemiological surveillance of yellow fever allowing the implementation of prevention actions, even before the occurrence of epizootics.

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“…The use of the biotin-avidin enzymelinked immunosorbent assay (ELISA) method allows the detection of small volumes of blood from single or various sources 6,[7][8][9] . The aim of the present study was to report on the feeding patterns of Nyssomyia intermedia (Ny.…”

Section: Introductionmentioning

confidence: 99%

Blood feeding patterns of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae) in a cutaneous leishmaniasis endemic area of the Ribeira Valley, State of São Paulo, Brazil

Marassá

Galati

Bergamaschi

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:The aim of this study was to identify the blood feeding sources of Nyssomyia intermedia (Ny. intermedia) and Nyssomyia neivai (Ny. neivai), which are Leishmania vectors and the predominant sandfl y species in the Ribeira Valley, State of São Paulo, Brazil, an endemic area for cutaneous leishmaniasis. Methods: Specimens were captured monthly between February 2001 and December 2003 on a smallholding and a small farm situated in the Serra district in the Iporanga municipality. The blood meals of 988 engorged females were tested using the avidin-biotin immunoenzymatic enzyme-linked immunosorbent assay (ELISA). Seven blood meal sources were investigated: human, dog, chicken, bovine, pig, horse and rat. Results: The results showed that among the females that fed on one or more blood sources, the respective percentages for Ny. intermedia and Ny. neivai, respectively, were as follows: human (23% and 36.8%), pig (47.4% and 26.4%), chicken (25.7% and 36.8%) and dog (3.9% and 0%), and the differences in the blood sources between the two species were statistically signifi cant (p = 0.043). Conclusions: Both species had predominant reactivity for one or two blood sources, and few showed reactivity indicating three or four sources. Many different combinations were observed among the females that showed reactivity for more than one source, which indicated their opportunistic habits and eclecticism regarding anthropic environmental conditions.

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Padronização da técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina para a identificação de sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912)

Cited by 15 publications

References 20 publications

Identificação do sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912) e Lutzomyia (Lutzomyia) almerioi (Galati & Nunes, 1999) pela técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina

Identificação do sangue ingerido por Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912) e Lutzomyia (Lutzomyia) almerioi (Galati & Nunes, 1999) pela técnica imunoenzimática do ELISA de captura, no sistema avidina-biotina

Circulation of antibodies against yellow fever virus in a simian population in the area of Porto Primavera Hydroelectric Plant, São Paulo, Brazil

Blood feeding patterns of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae) in a cutaneous leishmaniasis endemic area of the Ribeira Valley, State of São Paulo, Brazil

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