Phenetic studies on randomly amplified polymorphic DNA-polymerase chain reaction-variability of four geographical populations of Lutzomyia whitmani (Diptera: Psychodidae) in Brazil

Souza, Carina Margonari de; Fortes-Dias, Consuelo Latorre; Linardi, Pedro Marcos; Dias, Edelberto Santos

doi:10.1590/s0037-86822004000200007

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…Balbino 24 found a moderate genetic divergence among populations of Lu. longipalpis in seven municipalities in Northeastern Brazil, while, Souza et al 20 found that Ny. whitmani populations of four localities in the municipality of Corte de Pedra, State of Bahia, were more homogeneous than sandfly populations of Ilhéus (Bahia), Serra de Baturité (Ceará), and Martin Campos (Minas Gerais).…”

Section: Discussionmentioning

confidence: 94%

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Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil

Gasparotto

Costa-Ribeiro

Soccol

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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The genetic study of sandfly populations needs to be further explored given the importance of these insects for public health. Were sequenced the NDH4 mitochondrial gene from populations of Nyssomyia neivai from Doutor Camargo, Lobato, Japira, and Porto Rico, municipalities in the State of Paraná, Brazil, to understand the genetic structure and gene flow. Eighty specimens of Ny. Neivai were sequenced, 20 from each municipality, and 269 base pairs were obtained. A total of 27 haplotypes and 28 polymorphic sites were found, along with a haplotypic diversity of 0.80696 and a nucleotide diversity of 0.00567. Haplotype H5, with 33 specimens, was the most common among the four populations. Only haplotypes H5 and H7 were present in all four populations. The population from Doutor Camargo showed the highest genetic diversity, and only this population shared haplotypes with those from the other municipalities. The highest number of haplotypes was sheared with Lobato which also had the highest number of unique haplotypes. This probably occurred because of constant anthropic changes that happened in the environment during the first half of the twentieth century, mainly after 1998. There was no significant correlation between genetic and geographical distances regarding these populations. However, the highest genetic and geographical distances, and the lowest gene flow were observed between Japira and Porto Rico. Geographical distance is a possible barrier between these municipalities through the blocking of haplotype sharing.

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Section: Discussionmentioning

confidence: 94%

“…intermedia shows a greater genetic diversity in the wild than in domestic environments 18 , 19 . Souza et al 20 observed that Ny. whitmani has a high polymorphism and this is associated with the species’ adaptability to different regions.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil

Gasparotto

Costa-Ribeiro

Soccol

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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“…These arrangements of specimens from ecotypes that differ from those in which they were prevalent can also be justified by the existence of migration and gene flow. After evaluating the genetic variability of four populations of Lutzomyia (Nyssomyia) whitmani (Antunes and Coutinho, 1939) from different areas of ACL transmission in Brazil using RAPD, Souza et al (2004a) reported two main spatial groupings with a high level of intra-population variability: Corte de Pedra, Ilhéus (Bahia) and Serra de Baturité (Ceará) in the first group, and Martinho Campos (Minas Gerais) in the second. The vast majority of individuals of these populations were grouped according to their region of origin.…”

Section: Discussionmentioning

confidence: 99%

GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL

Gonçalves¹,

Castro

Luz

et al. 2021

Acta biol. Colomb.

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Lutzomyia intermedia (Diptera: Psychodidae) features as one of the main vectors that are involved in the transmission of American cutaneous leishmaniasis (ACL) in the Neotropical region. However, genetic studies involving this taxon are still incipient and important for understanding the level of variability of different populations, their role, and implications as vectors. The aim of this study was to determine the level of genetic diversity of L. intermedia present in the Ribeira River Valley, an area of ACL transmission in the state of Paraná, Brazil, through the Random amplified polymorphic DNA (RAPD). Two municipalities were chosen to collect sand flies: Cerro Azul (new transmission area of the ACL) and Adrianópolis (endemic area of the ACL). The insects were captured in the house, in the peridomicile and in the wild (forest). Two of the used markers made it possible to estimate the polymorphism of the studied populations, resulting in 40 genotypes, most of them from peridomicile. The dendrogram generated by the analysis with the primer A10 showed different degrees of similarity, suggesting that there may be gene flow in the studied populations. The Principal Coordinate Analysis (PCO) with the A2 primer, was useful in grouping L. intermedia according to its ecological and geographical origin. There was no distinction between the lineages composing the L. intermedia complex. The results of this study, with the record of great genotypic diversity in L. intermedia, may contribute to explain the maintenance of the life cycle of Leishmania braziliensis (Kinetoplastida: Trypanosomatidae) in the region.

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“…Despite their limitations, analyses by RAPD‐PCR have been widely used to evaluate degrees of heterogeneity in sandfly species (Adamson et al ., 1993; Dias et al ., 1998; Martin‐Sanchez et al ., 2000; de Souza et al ., 2004; Margonari et al ., 2004). This methodology has also been used for population studies of other vectors of human pathogens, such as mosquitoes and triatomines, and the information obtained has provided backing for discussions on the taxonomic status of species (Monteiro et al ., 2001).…”

Section: Discussionmentioning

confidence: 99%

Micro‐geographical variation among male populations of the sandfly,Lutzomyia(Nyssomyia)intermedia, from an endemic area of American cutaneous leishmaniasis in the state of Rio de Janeiro, Brazil

Meneses¹,

Cupolillo²,

Monteiro

et al. 2005

Medical Vet Entomology

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The genetic relationships among male Lutzomyia (Nyssomyia) intermedia (Lutz & Neiva) (Diptera: Psychodidae) from three populations from the same endemic area of American cutaneous leishmaniasis (ACL) in the state of Rio de Janeiro, Brazil, were compared. The sandflies were collected in three ecologically different habitats: domestic, extra-domestic and sylvatic over a total range of 800 m. Three molecular markers were employed to assess population variation. Based on MLEE markers, it could not be concluded that the three populations do not belong to the same gene pool (F(st) = 0.005). No within-population departure from Hardy-Weinberg equilibrium was detected (P < 0.05) and they presented the same level of gene variation. The number of migrants (Nm) indicated that at least 50 individuals per generation migrated between the three habitats. RAPD-PCR markers revealed that, except for the primer five, all were polymorphic. Phenetic analysis of the genotypes showed the presence of two principal clusters corresponding to: (1) domestic plus extra-domestic and (2) sylvatic. Unique genotypes were observed in each population. The sylvatic population was the most polymorphic, showing the largest number of genotypes and low level of similarity between them. Three mtDNA gene markers were studied by SSCP analysis. The most frequent haplotype for each marker ranged in frequency from 60 to 87% and individuals with unique haplotypes varied from 1 to 5%. Interestingly, the SSCP analysis showed a low level of polymorphism within populations. The disagreement between the different molecular markers observed and the hypothesis that L. intermedia could be participating in the transmission cycle of Leishmania (Viannia) braziliensis in environments ranging from the interior of human dwellings to the forest, are discussed.

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Phenetic studies on randomly amplified polymorphic DNA-polymerase chain reaction-variability of four geographical populations of Lutzomyia whitmani (Diptera: Psychodidae) in Brazil

Cited by 5 publications

References 22 publications

Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil

Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil

GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL

Micro‐geographical variation among male populations of the sandfly,Lutzomyia(Nyssomyia)intermedia, from an endemic area of American cutaneous leishmaniasis in the state of Rio de Janeiro, Brazil

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