Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones

Camandaroba, Edson Luiz Paes; Campos, Regina Helena de Freitas; Magalhães, Juracy B.; Andrade, Sônia Gumes

doi:10.1590/s0037-86822001000200001

Cited by 16 publications

(25 citation statements)

References 13 publications

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“…However, parasites were repeatedly observed in heart cells and skeletal muscle during the chronic phase, in association with inflammatory cell infiltrates. The predilection of Nicaragua parasites for cardiac and skeletal muscle in the chronic infected mice is similar to what has been described for other lineage I T. cruzi parasite infections (Botero et al, 2007; Camandaroba et al, 2001). Other authors described T. cruzi lineage I nests in endomyocardial biopsies of a chronic chagasic patient with end-stage heart failure (Texeira et al, 2006) and also reported to cause mainly chagasic cardiomyopathy (Toledo et al, 2002).…”

Section: Discussionsupporting

confidence: 81%

Trypanosoma cruzi: Biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs

Grosso¹,

Búa²,

Perrone³

et al. 2010

Experimental Parasitology

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We describe some biological and molecular characteristics of a Trypanosoma cruzi isolate derived from a Triatomine captured in Nicaragua. PCR based typification showed that this isolate, named Nicaragua, belonged to the lineage Tc I. Nicaragua infected culture cells were treated with allopurinol, showing different behaviour according to the cellular compartment, being cardiomyocyte primary cultures more resistant to this drug. The course of the infection in a mice experimental model and its susceptibility to benznidazole and allopurinol was analysed. In benznidazole treatment, mice reverted the high lethal effect of parasites during the acute infection, however, a few parasites were detected in the heart of 88 % of mice one year post-infection. Since Trypanosoma cruzi is a heterogeneous species population it is important to study and characterize different parasites actually circulating in humans in endemic areas. In this work we show that T. cruzi Nicaragua isolate, is sensitive to early benznidazole treatment.

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Section: Discussionsupporting

confidence: 81%

Trypanosoma cruzi: Biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs

Grosso¹,

Búa²,

Perrone³

et al. 2010

Experimental Parasitology

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“…Por otro lado, se ha encontrado que tanto en Chile como en otros países del Cono Sur, donde se sabe que prevalece el grupo T. cruzi II, predominan las formas digestivas de la enfermedad (7), mientras que en Colombia la manifestación clínica Nuestros resultados concuerdan con lo planteado por N. Añez y col (16), quienes encontraron que la infección con T. cruzi I presenta parasitemias mucho más altas que las causadas por T. cruzi II y que, además, T. cruzi I genera en pacientes durante la fase crónica un alto grado de daño en miocardio y músculo esquelético (34). Por el contrario, otros autores han encontrado que las infecciones con T. cruzi II son bastante graves y que comprometen algunos tejidos como recto y esófago (1,16,31).…”

Section: Cuadrounclassified

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Botero¹,

Mejía²,

Triana³

2007

biomedica

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Introducción. T. cruzi I y T. cruzi II son grupos genéticamente diferentes y se cree que dicha variabilidad es determinante del tropismo tisular en el hospedero vertebrado y responsable de las diversas manifestaciones clínicas de la enfermedad de Chagas. Objetivo. Caracterizar biológica y genéticamente dos clones colombianos de los grupos T. cruzi I y II en el modelo murino. Materiales y métodos. Las cepas CAS15 y AF1 pertenecientes a los grupos T. cruzi I y II fueron clonadas en medio semisólido. Un clon de cada una de ellas y una mezcla de ambos se utilizaron para infectar ratones, los cuales se sacrificaron a diferentes tiempos post-infección. Para analizar la presencia del parásito en sangre y diferentes órganos, se utilizaron el microhematocrito y la reacción en cadena de la polimerasa con los marcadores de la secuencia satélite del ADN nuclear y con el espaciador intergénico del gen mini-exón. Resultados. El clon T. cruzi I fue más infectivo, observándose un tropismo preferencial por corazón, recto y músculo esquelético, mientras que el clon T. cruzi II presentó un tropismo preferencial por bazo e hígado. Durante la infección con la mezcla de los clones, se observó que el clon T. cruzi I predominó sobre el T. cruzi II tanto en sangre como en órganos. Conclusiones. Los resultados confirman que las diferencias genéticas entre los grupos de T. cruzi podrían estar determinando el tropismo tisular y de esta manera jugar un papel fundamental en el entendimiento de las manifestaciones clínicas de la enfermedad de Chagas en Colombia.Palabras claves: Trypanosoma cruzi, enfermedad de Chagas, variación (Genética), tropismo. Biological and genetic characterization of two Colombian clones of Trypanosoma cruzi groups I and IIIntroduction. Genetic differences between T. cruzi I and T. cruzi II may determine differences in their tissue tropism in the vertebrate host and may also be responsible for the differences in clinical manifestations of Chagas disease. Objective. Two Colombian clones of the T. cruzi groups I and II were characterized biologically and genetically in a murine model. Materials and methods. Strains Cas15 and AF1 belonging to the T. cruzi groups I and II were cloned in semisolid medium. A clone of each strain and a mix of both were used to infect mice; the mice were subsequently sacrificed at selected post-infection intervals. In order to identify the parasite presence in blood and organs, two methods were used (a) microhematocrit and (b) polymerase chain reaction with primers for satellite DNA and the intergenic region of miniexon. Results. The T. cruzi I clone was more infectious, with a preferential tropism observed in heart, rectum and skeletal muscle, whereas clone T. cruzi II exhibited a preferential tropism for spleen and liver. During the infection with the clone mixture a predominance of the T. cruzi I clone over clone II in blood as well as in organs was observed. Conclusion. The results corroborate that the genetic differences between the T. cruzi groups correlate with their tissue tropism, an...

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“…2001), it has been shown that clones isolated from the Colombian strain at the peak of parasitemia (30th day of infection) disclosed phenotypic homogeneity and high similarity with the parental strain, high resistance to chemotherapy (Camandaroba et al . 2003) as well as the same histotropism (Camandaroba et al . 2001).…”

mentioning

confidence: 92%

Trypanosoma cruzi: clones isolated from the Colombian strain, reproduce the parental strain characteristics, with ubiquitous histotropism

Camandaroba

Pessina

Andrade

2006

Int J Experimental Path

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Clonal histotropism and biological characters of five clones isolated during the early acute phase of the infection of Swiss mice with the Colombian strain of Trypanosoma cruzi (T. cruzi I), Biodeme Type III, were investigated. Clones were isolated from mice at the 10th and the 30th day of infection with the Colombian strain. Isolation was performed by micromanipulation and injection of one trypomatigote blood form into newborn mice, followed by passages into suckling mice for obtaining the inocula for the experimental groups. Mice infected with parental strain were also studied. All the clones have shown the basic characteristics of Biodeme Type III, with the same patterns of parasitemia, tissue tropism, morphological characters and isoenzymic profiles, such as the parental strain. Histotropism was most intense to myocardium and skeletal muscles, with intense lesions found in the advanced phase (20th to 30th day of infection). Both parental strain and the clones were seen to parasitize several organs and tissues; amastigote nests were identified in the cytoplasm of macrophages, adipose cells, smooth muscle of intestinal wall and Auerbach's neuronal plexus. The findings of the present study confirm the homology of the clones isolated from the Colombian strain, with predominance of a 'principal clone' and an ubiquitous distribution of parasites belonging to a same clone.

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Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones

Cited by 16 publications

References 13 publications

Trypanosoma cruzi: Biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs

Trypanosoma cruzi: Biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Trypanosoma cruzi: clones isolated from the Colombian strain, reproduce the parental strain characteristics, with ubiquitous histotropism

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