Contribuição ao diagnóstico morfológico da adiaspiromicose pulmonar

Abstract

The diagnosis of adiaspiromycosis is usually based on lung sections stained by hematoxylin-eosin, periodic acid Schiff and methenamine silver. Authors describe the fungus aspect examined by mucicarmin, picro-sirius and Congo red methods, including polarized light microscopy. In doubtful cases, these methods could contribute to histopathological diagnosis of Emmonsia parva var crescens.

Pulmonary adiaspiromycosis – yet a challenging condition?

“…Finally, comments are added about the Brazilian contribution to the morphologic diagnosis of pulmonary adiaspiromycosis utilizing mucicarmin, picro-sirius, and Congo red, in addition to the routine methods. By mucicarmine the inner and middle layers of the wall are discreetly positive, without birefringence to polarized light; with picro-sirius the wall has orange birefringence to polarized light; by red Congo there are more than three layers in the wall, with intense yellowish birefringence in polarized light; and phase contrast microscopy may reveal clear trilaminar wall structure, even in H & E 5 . Data herein included can be useful to solve diagnostic challenges mainly in low-income regions where more expensive resources are not available in daily practice.…”

“…In this scenery, one must emphasize the growing number of reports about lung adiaspiromycosis affecting diverse mammals and other animals all over the world [1][2][3][4][5] . Hughes K and Borman AM (2018) described an Oryctolagus cuniculus with pulmonary and tracheobronchial lymph node adiaspiromycosis and reviewed the related literature 3 .…”