Enzimotipagem de espécies do gênero Candida isoladas da cavidade bucal

Abstract

The production of phospholipase and proteinase exoenzymes was evaluated in seventy nine samples of Candida isolated from the oral cavity of patients with oral lesions characteristic of candidosis and from individuals presenting a clinically normal mouth, attended at the University of Dentistry of Ribeirão Preto USP. Among the strains of C. albicans isolated from oral lesions, the phospholipase and proteinase were detected in 83.3% and 66.7%, respectively. C. tropicalis and C. parapsilosis produced only proteinase. Regarding the isolated strains from niches without lesions, out of a total of 32 C. albicans, 71.9% presented phospholipase and 68.7% proteinase. C. tropicalis only presented the enzyme proteinase, C. glabrata, C. krusei, C. guilliermondii and Candida spp did not present any of the exoenzymes. Among the samples of C. albicans from both groups, the enzymotype 22 (positive phospholipase and proteinase weakly positive), was prevalent. Different enzymotypes of the same species were detected in samples collected from the same patient.

Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil

“…This finding differs from other authors and corroborates the results of other studies in which phospholipase activity was detected in C. albicans, C. parapsilosis, C. tropicalis, and C. guilliermondii (35,36). Enzymatic activity has been detected in yeasts isolated from biological samples from different anatomical origins (anus, vagina, and mouth) in 17 patients (37,38).…”

“…Some studies also found that certain species of yeasts can produce proteases whereas others cannot, which may contribute to the increased virulence.However, in numerous cases of vaginal infection caused by yeasts proteinase activity is not detected. This may indicate that the secretion of protease is not the only factor involved in the ability of causing vulvovaginitis (35,36).…”

Phenotypic aspects of oral strains of Candida albicans in children with down's syndrome

“…Nevertheless, the individual analysis of the productive enzyme capacity of each strain of C. albicans revealed that strains from the mouth of children with Down's syndrome showed enhanced production. Cândido et al (2000) studied 79 Candida strains isolated from the mouth of patients with lesions characteristic of candidiasis and from individuals with clinically normal oral cavity treated at the School of Dentistry in Ribeirão Preto, universidade de São Paulo (FO/RP/uSP), brazil, and found out that 83.3% and 66.7% of C. albicans strains from oral lesions produced phospholipase and proteinase, respectively. However, the strains of this yeast obtained from the mouth of individuals with no lesions produced phospholipase in 71.9% and proteinase in 68.7% of the cases.…”

“…Among the strains of C. albicans in both groups, enzymetype 22 (positive phospholipase and weakly positive proteinase) was the most prevalent. This showed that the phospholipidolytic capacity of oral strains of C. albicans is a preponderating factor for candidiasis (Cândido et al, 2000;Ribeiro et al, 2002). ghannoum & Abu-Elteen (1990) and Cândido et al (2000) affirm that these hydrolytic enzymes produced by Candida yeasts are virulence factors for this yeast-like fungus.…”

“…Therefore, the phenotypic characterization of yeast-like fungi enables us to assess macroscopically how these microorganisms behave as a constituent part of the topical microbiota and/or as the triggering mechanism of candidiasis regarding morphological, serological, biological, and antifungal parameters (Prince et al, 1982;ghannoum & Abu-Elteen, 1990;Odds, 1998;Cândido et al, 2000;Pinto, 2003).…”