Aplicação de método de coloração tricrômica, em fezes diarréicas de infectados pelo HIV, para pesquisa de microsporídios

Abstract

The microsporidia have been involved in several clinical manifestations in patients with AIDS, of whom diarrhoea is the commonest. The diagnosis of microsporidiasis depended on invasive procedures and the identification of the organisms is made by electron microscopy. The modified trichrome staining method allows that the diagnosis be made without such procedures by using light microscopy. In the present work, the modified trchrome method was applied in stools from 62 patients with diarrhoea, who had asymptomatic HIV infection or AIDS. Of the 62 samples analyzed, there was detection of microsporidial spores in one. This work confirms the existence of such protozoans in our patients, associated with manifestations of chronic diarrhoea in patients with AIDS who have severe immunodeficiency and ascertains that this staining method allows satisfactory identification of microsporidia from faeces, as well points out some directions to further studies.

Infection by intestinal parasites in disabled patients and their guardians

“…The Hoffman-Pons-Janer 8 , centrifugal flotation in zinc sulfate solution 9 and Rugai 10 methods were used to prepare samples. In addition, Gram-Chromotrope 11 , Leishman 12 , Kinyoun 13 , Kato-Katz 14 e trichrome 15 staining techniques were used for protozoan research. Two slides were examined for each stool sample and method to detect parasites using optical microscopy (Nikon, Japan) with magnifications of 100x, 400x and 1000x.…”