Emergência da múltipla resistência a antimicrobianos em Vibrio cholerae isolados de pacientes com gastroenterite no Ceará, Brasil

Hofer, Ernesto; Quintaes, Bianca Ramalho; Reis, Eliane Moura Falavina dos; Rodrigues, Dália dos Prazeres; Seki, Liliane Miyuki; Feitosa, I.S.; Ribeiro, L.H.F.F.; Ferreira, Maria Rozzelê

doi:10.1590/s0037-86821999000200006

Cited by 6 publications

(5 citation statements)

References 20 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise , Hofer et al (1999, while working on isolates of V. cholerae and Escherichia coli, observed the ability they have to transfer this resistance on to other bacterial families through transference of multiresistant plasmid. Randrianarivelo et al (2010) recommend that the antimicrobials should be restricted only to therapeutic use and that preventive focus can be more expensive than posterior treatments.…”

Section: Resultsmentioning

confidence: 99%

Pathogenic Vibrio species isolated from estuarine environments (Ceará, Brazil) - antimicrobial resistance and virulence potential profiles

Menezes

Rodríguez

Carvalho

et al. 2017

An. Acad. Bras. Ciênc.

Self Cite

View full text Add to dashboard Cite

Detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. Thus, Vibrio diversity was investigated in four estuaries from state of Ceará (Pacoti, Choró, Pirangi and Jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. Isolation and identification were performed on TCBS agar (selective medium) and dichotomous key based on biochemical characteristics, respectively. Nineteen strains of genus Vibrio were catalogued. Vibrio parahaemolyticus (Choró River) and V. alginolyticus (Pacoti River) were the most abundant species in the four estuaries. All strains were submitted to disk diffusion technique (15 antimicrobials were tested). Resistance was found to: penicillin (82%), ampicillin (54%), cephalotin (7%), aztreonan (1%), gentamicin, cefotaxime and ceftriaxone (0.5%). Five pathogenic strains were chosen to verification of virulence factors. Four estuaries showed a high abundance of species. High number of tested positive strains for virulence is concerning, since some of those strains are associated to human diseases, while others are known pathogens of aquatic organisms.

show abstract

Section: Resultsmentioning

confidence: 99%

Pathogenic Vibrio species isolated from estuarine environments (Ceará, Brazil) - antimicrobial resistance and virulence potential profiles

Menezes

Rodríguez

Carvalho

et al. 2017

An. Acad. Bras. Ciênc.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Multidrug resistance in V. cholerae O1 isolates has so far been linked to plasmids, and the presence of self-transmissible multidrug-resistant plasmids from 100 to 200 kb has been reported in V. cholerae O1 isolates from Asia, Africa, and Ecuador (10,14,19,33,40,41,43) more recently, in isolates from Albania, Italy, and Brazil (11,15). However, plasmids such those harbored by isolates M1516 and M3030, which encode resistance to gentamicin, kanamycin, amikacin, streptomycin, trimethoprim-sulfamethoxazole, tetracycline, and chloramphenicol and, in the case of M1516, to erythromycin, in addition to ␤-lactam antibiotic resistance, have not previously been reported.…”

Section: Discussionmentioning

confidence: 99%

Plasmidic Extended-Spectrum β-Lactamases in Vibrio cholerae O1 El Tor Isolates in Argentina

Petroni

Corso

Melano

et al. 2002

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Since 1992 there have been seven major outbreaks of cholera in Argentina. Susceptibility analysis of 1,947 isolates (40% of reported cases) of Vibrio cholerae O1 biotype El Tor suggested the presence of extendedspectrum ␤-lactamases (ESBLs) in 28 isolates. Because of their different susceptibility profiles, V. cholerae isolates M1502, M1516, M1573, and M3030 (all of which are of the Ogawa serotype) were selected for the present study. By susceptibility analysis, isoelectric focusing, and PCR-based restriction fragment length polymorphism analysis, CTX-M-type enzymes were identified in three isolates, whereas a PER-2-type enzyme, in addition to a TEM-1-like enzyme, was identified in the other isolate. The presence of these ESBLs in V. cholerae isolates resulted in MICs well below those commonly observed for members of the family Enterobacteriaceae. Genes that encode both ESBLs were transferred to Escherichia coli by conjugation, together with all determinants of resistance to non-␤-lactam antibiotics (gentamicin, kanamycin, and sulfamethoxazole for all isolates; amikacin and streptomycin for three isolates; trimethoprim, tetracycline, and chloramphenicol for two isolates). Plasmid profile analysis and Southern blotting revealed the presence of single plasmids of about 150 kb in the four V. cholerae isolates and their respective transconjugants and revealed that the plasmids harbored genes encoding CTX-M-type or PER-2-type ESBLs. These results strongly suggest the broad spread of these ESBLs among genera belong to families other than the Enterobacteriaceae.

show abstract

“…In addition to the development of multi-drug resistant strains of bacteria which is also another growing problem [1-3]. All necessitates the search for alternate therapies to fight infections [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Microbiological Assessment of Moringa Oleifera Extracts and Its Incorporation in Novel Dental Remedies against Some Oral Pathogens

Elgamily

Moussa

Elboraey

et al. 2016

Open Access Maced J Med Sci

View full text Add to dashboard Cite

AIM:To assess the antibacterial and antifungal potentials of different parts of Moringa oleifera plant using different extraction methods in attempts to formulate natural dental remedies from this plant.MATERIAL AND METHODS:Three solvents extracts (Ethanol, acetone, and ethyl acetate) of different parts of Egyptian Moringa tree were prepared and tested against oral pathogens: Staphylococcus aureus, Streptococcus mutans, and Candida albicans using disc diffusion method; As well as to incorporate the plant extract to formulate experimental toothpaste and mouthwash. The two dental remedies were assessed against the same microbial strains. Statistical analysis was performed using One-Way ANOVA test to compare the inhibition zone diameter and t-test.RESULTS:Ethanol extracts as well as leaves extracts demonstrated the highest significant mean inhibition zone values (P ≤ 0.05) against Staphylococcus aureus and Streptococcus mutans growth. However, all extracts revealed no inhibition zone against Candida albicans. For dental remedies, experimental toothpaste exhibited higher mean inhibition than the mouthwash against Staphylococcus aureus, Streptococcus mutans and only the toothpaste revealed antifungal effect against Candida albicans.CONCLUSION:The different extracts of different parts of Moringa showed an antibacterial effect against Staphylococcus aureus and Streptococcus mutans growth. The novel toothpaste of ethanolic leaves extract has antimicrobial and antifungal potential effects all selected strains.

show abstract

Emergência da múltipla resistência a antimicrobianos em Vibrio cholerae isolados de pacientes com gastroenterite no Ceará, Brasil

Cited by 6 publications

References 20 publications

Pathogenic Vibrio species isolated from estuarine environments (Ceará, Brazil) - antimicrobial resistance and virulence potential profiles

Pathogenic Vibrio species isolated from estuarine environments (Ceará, Brazil) - antimicrobial resistance and virulence potential profiles

Plasmidic Extended-Spectrum β-Lactamases in Vibrio cholerae O1 El Tor Isolates in Argentina

Microbiological Assessment of Moringa Oleifera Extracts and Its Incorporation in Novel Dental Remedies against Some Oral Pathogens

Contact Info

Product

Resources

About