Effect of a booster-dose of rabies vaccine on the duration of virus neutralizing antibody titers in bovines

Albas, Avelino; Pardo, Paulo Eduardo; Gomes, Albério A. B.; Bernardi, Fernanda; Ito, Fumio

doi:10.1590/s0037-86821998000400006

Cited by 15 publications

(17 citation statements)

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“…However, single doses of rabies vaccines have been associated with low levels of neutralizing antibodies and also with occurrence of disease in vaccinated animals (9,15). On the other hand, the use of a booster has significantly increased antibody specific levels (2,6,10,14,17).…”

Section: Introductionmentioning

confidence: 99%

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies

Albas

Fontolan

Pardo

et al. 2006

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:In this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three alternative immunization procedures. Forty-five bovines (breed nelore) were then organized in three groups (A, B and C, with 15 animals/group). Group A received only one vaccine dose at day zero and Group B received the first dose at day zero and then another dose at day 30 (early booster). Group C was also immunized with two doses; however, the booster was postponed until day 180 after the first dose (delayed booster). Blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390, and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. The protocol used to immunize Group C (booster at day 180) was clearly more efficient. In this group, antibody levels were higher and also remained higher for longer periods in comparison with the other two groups. These results show that booster timing significantly affected antibody levels. Therefore, programs addressed to control this disease in cattle should consider not only the use of a booster but also its administration time.

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Section: Introductionmentioning

confidence: 99%

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies

Albas

Fontolan

Pardo

et al. 2006

J. Venom. Anim. Toxins incl. Trop. Dis

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“…To be considered immune, an individual requires antibody titers ≥ 0.5 IU ml -1 (international units per milliliter) (WHO, 1992) and a number of studies have suggested that this dose should also be applied as the minimum for protection in cattle (SIHVONEN et al, 1994;ALBAS et al, 1998;RODRIGUES DA SILVA et al, 2000;ALBAS et al, 2005). Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, factors such as the quality of the vaccine, the physiological state of the animals and the individual capacity of the immune system response can all contribute to a reduction in the efficiency and effectiveness of vaccination in cattle herds. It should be highlighted that despite the variation in bovine responses, the vaccines used were all properly released, registered and approved by the Ministry of Agriculture, Livestock and Food Supply (ALBAS et al, 1998;GIOMETTI et al, 2006;MARIA et al, 2009). …”

Section: Frequency Of Cattle With Titers Of Antirabies Antibodiesmentioning

confidence: 99%

Efeito da concentração de bactérias probióticas como imunomodulador da produção de anticorpos antirrábicos em bovinos vacinados

et al. 2016

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This study evaluated the effects of supplementation with a combination of probiotic microorganisms, added at different concentrations to the mineral mixture, on the production of serum antibodies against rabies virus in cattle vaccinated with a single dose of rabies vaccine. Forty-two male Nellore cattle, aged 12 months, were randomly divided into three groups (n = 14): the control group (CG) received 70 grams of mineral mixture/animal/day; and the 2 gram probiotic group (G2P) and 8 gram probiotic group (G8P) received 70 grams of mineral mix/animal/day with 2 and 8 grams added, respectively, of a combination of probiotic microorganisms (Lactobacillus acidophilus, Streptococcus faecium, Bifidobacterium thermophilum and Bifidobacterium longum). Individual antibody titers were determined using a neutralization in cell-based rapid fluorescent focus inhibition test (RFFIT) technique. One-way analysis of variance (one-way ANOVA) was used with contrasts using the Tukey method to determine whether the experimental groups differed within each time point, and the paired t-test was used to determine whether differences occurred between time points within each group. The level of significance was set at 5%. There were statistically significant differences between the mean serum concentrations of the CG and G8P groups at 30 and 60 days after the first vaccination, and at 60 days, 100% of the animals maintained minimum titers of protective antibodies only in the G8P group. There was also improvement in the production of antibodies in the G2P group compared with the CG after 30 and 60 days, but this difference was not statistically significant. In conclusion, increasing doses of probiotic added to the mineral mix beneficially affected the rabies humoral immune response, as determined by serum antibodies, and enabled the maintenance of minimum protective titers for a longer period in previously vaccinated cattle. ResumoEsse estudo avaliou o efeito da suplementação de uma associação de microrganismos probióticos, adicionados à mistura mineral em diferentes doses, na produção de anticorpos séricos antirrábicos em bovinos primovacinados. Quarenta e dois bovinos Nelore machos, com idade de 12 meses, foram divididos em três grupos (n=14): grupo controle (GC) recebeu 70 gramas de mistura mineral/animal/dia; grupos probiótico 2 gramas (G2P) e 8 gramas (G8P) receberam 70 gramas de mistura mineral/animal/dia adicionados respectivamente de 2 e 8 gramas de probióticos (Lactobacillus acidophilus, Streptococcus faecium, Bifidobacterium thermophilum e Bifidobacterium longum). Os títulos individuais de anticorpos antirrábicos foram determinados por meio da técnica de soroneutralização em células baseado no rapid fluorescent focus inhibition test (RFFIT). Os resultados obtidos foram comparados pelo teste t não pareado, com 5% de nível de significância. Houve diferenças estatísticas significativas entre as médias de concentrações séricas entre os grupos GC e G8P, após 30 e 60 dias da primovacinação e após 60 dias, somente o G8P manteve...

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“…Primeiramente notou-se que a resposta imune humoral anti-rábica nos bovinos dos grupos GC e GP foi heterogênea, ficando confirmada pelo elevado coeficiente de variação (C.V.) dos títulos de anticorpos, para grupos (C.V.= 137,19%) e dias de observação (C.V.= 57,48%), apresentando animais que não responderam à vacinação (títulos de anticorpos <0,50 UI/mL) intercalados com outros que apresentaram títulos de anticorpos protetores mínimos (≥0,50 UI/mL) a elevados. Este fato também foi observado por Ciuchini et al (1981), Albas et al (1998Albas et al ( , 2005, Giometti et al (2006) e Maria et al (2007) avaliando a resposta imune humoral a outras vacinas anti-rábicas. Portanto, esta variação nos títulos de anticorpos nos bezerros pode ser uma característica fisiológica da resposta imune humoral anti-rábica em bovinos primovacinados.…”

Section: Resultsunclassified

“…Além disso, foi observada uma elevada porcentagem de bovinos (75,6%) protegidos. Esta redução nos títulos de anticorpos anti-rábicos é um efeito fisiológico da resposta imune humoral em bovinos primovacinados contra a raiva, pois este fato também foi observado por Albas et al (1998Albas et al ( , 2005, Giometti et al (2006) Apesar da vacina avaliada neste experimento ter excelente capacidade de induzir a soroconversão e de manter os títulos de anticorpos anti-rábicos considerados protetores nos bovinos primovacinados contra a raiva por longos períodos, estes resultados não dispensam a necessidade da aplicação da dose de reforço em bovinos primovacinados conforme preconizado pelo Programa de Controle da Raiva em Herbívoros do Instituto Pasteur.…”

Section: Resultsunclassified

Avaliação da vacinação anti-rábica e da suplementação com probiótico na resposta imune humoral em bovinos

et al. 2009

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ResumoObjetivou-se avaliar a resposta imune humoral a uma nova vacina anti-rábica, desenvolvida no Instituto Butantan em bovinos primovacinados e o efeito do probiótico nesta resposta. Trinta e quatro bovinos da raça Nelore com idade de 15 meses foram divididos aleatoriamente em 2 grupos (17 bovinos/grupo): os animais foram vacinados no dia zero e um dos grupos recebeu uma mistura mineral com probiótico (GP), enquanto o outro apenas a mistura (GC). Colheu-se sangue dos animais nos dias 0, 75 e 150 após a vacinação para determinação dos títulos de anticorpos anti-rábicos neutralizantes pela técnica de soroneutralização em células BHK 21 (RFFIT). Foram encontrados títulos de anticorpos protetores (≥0,5 UI/mL) em 82,4% dos animais do grupo GP e 76,5% do grupo GC. Não houve diferença significativa (p>0,05) nos títulos de anticorpos entre os soros coletados dos dois grupos de animais nos dias 75 e 150. Verificou-se também que para ambos os grupos no dia 150 houve uma redução significativa (p<0,01) nos títulos de anticorpos. Conclui-se que a vacina anti-rábica é eficiente em produzir soroconversão e em manter os títulos de anticorpos em bovinos primovacinados. A ingestão do probiótico não interferiu na resposta imune humoral anti-rábica. Palavras-chave: Probiótico, resposta imune humoral, vacina anti-rábica, anticorpos, bovino AbstractThis study evaluated the humoral immune response of a new rabies vaccine developed by the Instituto Butantan (potency of 3.27 UI/ml) in primovaccinated cattle and the effect of probiotic on this response. Thirty-four 15-month old Nelore cattle were randomly divided into 2 groups (17 animals/group). All the animals were vaccinated on day 0 (zero) and then animals in one group received probiotic added to a mineral mixture (GP) while the others were given only the mineral mixture (GC). Blood samples were collected on days 0, 75 and 150 for rabies neutralizing antibodies titers by seroneutralization assay on BHK 21 cells (RFFIT). Protective antibody titers (≥0.5 UI/mL) were found in 82.4% of the animals from GP and in 76.5% of the animals from GC and no statistical difference (p>0.05) between antibody titers in GP and GC was detected on days 75 and 150. It was also observed that in both groups antibody titers was decreased on day 150 (p<0.01). In conclusion, the tested rabies vaccine promotes efficient soroconversion and keeps antibody levels in primovaccinated cattle, but probiotic does not affect the humoral anti-rabies immune response.

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Effect of a booster-dose of rabies vaccine on the duration of virus neutralizing antibody titers in bovines

Abstract: Efeito de uma dose de reforço da vacina anti-rábica sobre a duração de títulos de anticorpos neutralizantes do vírus, em bovinos

Cited by 15 publications

References 1 publication

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies

Efeito da concentração de bactérias probióticas como imunomodulador da produção de anticorpos antirrábicos em bovinos vacinados

Avaliação da vacinação anti-rábica e da suplementação com probiótico na resposta imune humoral em bovinos

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