Multirresistência a antimicrobianos mediada por plasmídios R em cepas de Shigella flexneri isoladas no nordeste do Brasil

Sidrim, José Júlio Costa; Moreira, José Luciano Bezerra; Paixão, Germana Costa; Lima, Sergio Bezerra; Filho, Renato Evandro M.; Rocha, Marcos Fábio Gadelha; Lima, Aldo Ângelo Moreira

doi:10.1590/s0037-86821998000300003

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…strains isolated in Mozambique showed that the majority of the MDR isolates were from S. flexneri (47.7%) compared with 7.4% from other species [8]. In Brazil, Sidrim et al, 1998, studied the resistance of 26 S. flexneri strains isolated in the Northeast of Brazil and observed that all strains studied were MDR [45]. Penatti et al (2007) showed that 90% of the S. flexneri strains isolated in Campinas, State of São Paulo, Brazil, were MDR [46].…”

Section: Discussionmentioning

confidence: 99%

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

et al. 2020

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Shigella flexneri has been a major public health problem in developing countries. This work analyzed the frequency of 16 virulence genes, the genotypic diversity, and the antimicrobial resistance profiles of 130 S. flexneri strains isolated in Brazil. The ipaH gene was found in all the 130 strains. The frequencies of the other genes were variable ial (88.5%), sigA (82.3%), iuc (74.6%), virA (73%), pic (72.3%), virF (57.7%), sat (48.5%), ipaBCD (37%), sen (36%), set1A (35.4%), sepA (30%), set1B (30%), virB (14%), icsA (10%), and ipgD (5.4%). A total of 57 (43.8%) strains were multidrug-resistant. ERIC-PCR grouped 96 of the strains into a single cluster with ≥ 70.4% of similarity, 75 of these strains presented a similarity ≥ 80.9%. PFGE grouped 120 of the strains into a single cluster with 57.4% of similarity and 82 of these strains presented a similarity ≥ 70.6%. In conclusion, the high frequency of some virulence genes reinforces the pathogenic potential of the strains studied. The high rates of MDR strains are alarming once it may lead to failure when antimicrobial treatment is necessary. Genotype techniques reveled a major cluster with high genetic similarity including S. flexneri strains from the different Brazilian states and distinct years of isolation, showing that they probably emerged from a common ancestor.

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Section: Discussionmentioning

confidence: 99%

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

et al. 2020

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“…Important biological functions are associated with plasmids in Salmonella including virulence factors, resistance to heavy metals, antibiotics, phages and utilization of carbon sources [ 10 ]. The virulence plasmids usually have high molecular weight [ 11 ]. The dissemination of the plasmid may cause an increase in bacterial resistance [ 12 ], interfering with the clinical importance of infections by contributing to increased spread of antibiotic resistance genes [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Bacteriological water quality in school’s drinking fountains and detection antibiotic resistance genes

Freitas

Silva

Bataus

et al. 2017

Ann Clin Microbiol Antimicrob

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The fecal coliform can contaminate water of human consumption causing problems to public health. Many of these microorganisms may contain plasmid and transfer them to other bacteria. This genetic material may confer selective advantages, among them resistance to antibiotics. The objectives of this study were to analyze the presence of fecal coliforms in water and at drinker surface, to identify the existence of plasmid, conducting studies of resistance to antibiotics, plasmid stability and capacity of bacterial conjugation. Were collected microorganisms in water of drinker surface and were used specific culture media and biochemical tests for identification of organisms, tests were performed by checking the resistance to antibiotics (ampicillin 10 μg, tetracycline 30 μg, and ciprofloxacin 5 μg), was performed extraction of plasmid DNA, plasmid stability and bacterial conjugation. Was obtained results of 31% of Salmonella spp. and 51% for other coliforms. Among the samples positive for coliforms, 27 had plasmid stable and with the ability to perform conjugation. The plasmids had similar forms, suggesting that the resistance in some bacteria may be linked to those genes extra chromosomal.

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“…Likewise, Sidrim et al . () have demonstrated the transfer of plasmids containing ampicillin resistance genes from Shigella flexneri to Escherichia coli . In the present study, the level of copper resistance of transconjugants obtained with Cu T recipient strains was similar to that of the Cu R donor strains, whereas transconjugants obtained with Cu S recipient strains had a lower level of resistance than the Cu R donor strains.…”

Section: Discussionmentioning

confidence: 99%

Description of copper tolerant Xanthomonas citri subsp. citri and genotypic comparison with sensitive and resistant strains

et al. 2019

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The copper-based products widely used for control of citrus canker may lead to the development of Xanthomonas citri subsp. citri (X. citri) resistant to copper (Cu R ). However, the study of copper sensitivity of X. citri strains from Paran a state, Brazil, did not reveal the existence of Cu R , but copper tolerant (Cu T ) strains. The aim of this study was to describe for the first time the existence of Cu T X. citri and compare the genetic determinants that differentiate the Cu T strains from the sensitive (Cu S ) and Cu R strains. Cu T strains supported intermediate concentrations of copper in comparison to Cu S and Cu R . Cu T strains lack the gene clusters copLAB or copABCD responsible for copper resistance in Cu R strains and the large plasmids (c. ≥200 kb) that normally carry these genes. The nucleotide sequences of chromosomal homologous genes cohLAB, involved in copper homeostasis, were 100% similar in strains of all phenotypes. Cu T strains differed from Cu S strains by the higher expression of the homologous chromosomal genes cohA and cohB in the presence of copper. Cu T X. citri strains are not precursors of Cu R strains and do not pose a threat to the efficient use of copper-based bactericides for management of citrus canker in citrus orchards. Copper resistance and tolerance are distinct phenotypes and should not be used as synonyms. The proper characterization of the sensitivity to copper leads to a more confident monitoring of the distribution of copper resistant populations of X. citri and adoption of containment measures only when necessary.

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Multirresistência a antimicrobianos mediada por plasmídios R em cepas de Shigella flexneri isoladas no nordeste do Brasil

Cited by 5 publications

References 14 publications

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

Bacteriological water quality in school’s drinking fountains and detection antibiotic resistance genes

Description of copper tolerant Xanthomonas citri subsp. citri and genotypic comparison with sensitive and resistant strains

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