Teste de aglutinação direta no sorodiagnóstico da leishmaniose visceral no estado do Pará

Garcez, Lourdes Maria; Shaw, Jeffrey Jon; Sílveira, Fernando Tobias

doi:10.1590/s0037-86821996000200009

Cited by 14 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this meta-analysis included only two studies from Latin –America, both from Brazil, and both with small sample sizes. Garcez et al (1996) reported 100% sensitivity on 16 parasitologically confirmed VL cases and 98.3% specificity on a mixed group of 65 healthy endemic controls and patients with other diseases [67]. Schallig et al (2002) reported 100% sensitivity on 21 confirmed VL cases and 100% specificity on 19 healthy controls and 42 samples of patients with other diseases [68].…”

Section: Resultsmentioning

confidence: 99%

Control of Visceral Leishmaniasis in Latin America—A Systematic Review

2010

View full text Add to dashboard Cite

BackgroundWhile three countries in South Asia decided to eliminate anthroponotic visceral leishmaniasis (VL) by 2015, its control in other regions seems fraught with difficulties. Is there a scope for more effective VL control in the Americas where transmission is zoonotic? We reviewed the evidence on VL control strategies in Latin America—diagnosis, treatment, veterinary interventions, vector control—with respect to entomological and clinical outcomes.Methodology/Principal FindingsWe searched the electronic databases of MEDLINE, LILACS, and the Cochrane Central Register of Controlled Trials, from 1960 to November 2008 and references of selected articles. Intervention trials as well as observational studies that evaluated control strategies of VL in the Americas were included. While the use of rapid diagnostic tests for VL diagnosis seems well established, there is a striking lack of evidence from clinical trials for drug therapy and few well designed intervention studies for control of vectors or canine reservoirs.ConclusionElimination of zoonotic VL in the Americas does not seem a realistic goal at this point given the lack of political commitment, gaps in scientific knowledge, and the weakness of case management and surveillance systems. Research priorities and current strategies should be reviewed with the aim of achieving better VL control.

show abstract

Section: Resultsmentioning

confidence: 99%

Control of Visceral Leishmaniasis in Latin America—A Systematic Review

2010

View full text Add to dashboard Cite

show abstract

“…Antibody titers ≥ to 10 were detected in four (100%) crab-eating foxes (Cerdocyon Difficulties with wild animal capture, taxonomic identification and standardization of laboratory techniques for ACL diagnosis constitute restricting factors in the understanding of leishmaniasis epidemiology (17). Several authors, employing Leishmania promastigotes, had utilized DAT in epidemiological studies (32)(33)(34)(35). In fact, the test does not require special equipment or species-specific immunoglobulin conjugate, and may be useful in cases of natural and experimental infections (34).…”

Section: Search For Antibodies By Datmentioning

confidence: 99%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua. These results suggest that Cerdocyon thous and Lycalopex vetulus (crab-eating fox), Cebus apella (capuchin monkey), Dasyprocta azarae (agouti), Procyon cancrivorus (crab-eating raccoon) and Nasua nasua (coati) play an important role in the ACL transmission cycle in the northwestern region of Paraná, Brazil.

show abstract

“…Whether this slightly relatively low sensitivity is to be attributed to use of the heterologous Leishmania subspecies as antigen in the DAT is difficult to conclude based on this limited number of sera. Using an aqueous trypsinized antigen of L. donovani , Garcez et al (1996) reported much lower sensitivity (71.4 %) in Brazilian dogs with confirmed CVL. Terán-Angel et al (2007) suggested that the lower sensitivity encountered with the DAT in identifying dogs with genuine CVL is most likely associated with the use of a different Leishmania species lacking particular antigen characteristics unique to the strain that is endemic in the area wherein the study was carried out.…”

Section: Discussionmentioning

confidence: 94%

Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

Semião-Santos

Veloso

Andrade

et al. 2014

Journal of Medical Microbiology

View full text Add to dashboard Cite

Ahfad University for Women, Omdurman, SudanWe compared the performance of a locally produced b-mercaptoethanol-modified promastigote antigen (b-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for b-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for b-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen's k50.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of b-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs. INTRODUCTIONIn the Mediterranean region, visceral leishmaniasis (VL) is a zoonotic disease caused by Leishmania infantum. As in most northern European countries, many people in this region own dogs which, due to optimal habitat, attract phlebotomine flies, increasing the chance of disease transmission to humans and other animals (Gramiccia, 2011). Based on available data, it is assumed that among the existing 15 million dogs residing in southern Europe 2.5 million (16.7 %) are infected with Leishmania (Moreno & Alvar, 2002). Canine visceral leishmaniasis (CVL) seroprevalence rates varying from 1.7 % in southern Cyprus to more than 40 % in southern Italy have been reported (Deplazes et al., 1998;Oliva et al., 2006). More than half of these dogs present asymptomatic Leishmania infection and are thus considered as eligible for transmitting the disease (Molina et al., 1994;Michalsky et al., 2007). Unfortunately, none of the available serological tests has the required efficiency for detecting CVL at the early phase. This shortcoming has urged public health and veterinary authorities to seriously search for tools that are sensitive and reliable for detection of the disease at this phase (Rajasekariah et al., 2008). Establishing diagnostic methods 3Emeritus microbiologist.Abbreviations: (C)VL, (canine) visceral leishmaniasis; DAT, direct agglutination test; b-ME, b-mercaptoethanol.

show abstract

Teste de aglutinação direta no sorodiagnóstico da leishmaniose visceral no estado do Pará

Cited by 14 publications

References 20 publications

Control of Visceral Leishmaniasis in Latin America—A Systematic Review

Control of Visceral Leishmaniasis in Latin America—A Systematic Review

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

Contact Info

Product

Resources

About