Treatment of T. cruzi infected human platelet concentrates with aminomethyltrimethyl psoralen (AMT) and ultravioleta (UV-A) light: preliminary results

Moraes‐Souza, Helio; Bordin, José Orlando; Bardossy, L; Blajchman, Morris A.

doi:10.1590/s0037-86821996000100009

Cited by 4 publications

(1 citation statement)

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“…The described inactivation protocol therefore will eliminate infectivity in a 50 mL unit of PC. The observation that the trypomastigote motility is more resistant to PUVA treatment than loss of infectivity explains an earlier report that AMT is ineffective as a parasite-inactivating reagent (15). In that report, viability of parasites was evaluated using motility 24 h after treatment as an endpoint.…”

Section: Discussionmentioning

confidence: 97%

Inactivation of Trypanosoma cruzi Trypomastigote Forms in Blood Components with a Psoralen and Ultraviolet A Light

Gottlieb

Margolis‐Nunno

Robinson

et al. 1996

Photochem & Photobiology

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Inactivation of the blood-borne parasite Trypanosoma cruzi by UVA and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) was studied in the blood components fresh frozen plasma (FFP) and platelet concentrate (PC). The AMT was utilized at a concentration of 50 micrograms/mL and the inactivation procedure included the flavonoid rutin (at 0.35 mM), a quencher of type I and type photo-reactants, which we have previously found to maintain platelet integrity during this treatment regimen. Within both FFP and PC, complete inactivation of the infective form of T. cruzi, the trypomastigote, was achieved at a UVA (320-400 nm radiation) fluence of 4.2 J/cm2. We note that while the infectivity of the parasite is eliminated at 4.2 J/cm2 the trypomastigote motility continues for at least 16 h-post-treatment and is inhibited only after much higher light doses. Isolation of total DNA from the parasite cells after treatment in the presence of 3H-AMT indicated that at the lethal UVA influence about 0.5 AMT adducts per kilobase pairs occurred. These results suggest that this psoralen plus UVA methodology which shows promise in enhancing the viral safety of PC, may in addition eliminate bloodborne T. cruzi, the causative agent of Chagas disease.

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Section: Discussionmentioning

confidence: 97%