Estudo epidemiológico da infecção hospitalar e resistência a antimicrobianos e ao telurito em Enterobacteriaceae com referência especial a Klebsiella pneumonia

Pereira, José Augusto Adler

doi:10.1590/s0037-86821993000400014

Cited by 3 publications

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“…In the hospital environment related to this study, as well as in similar hospitals, this convergence in multiresistance is often associated to gentamicin resistance (Pereira 1990), both in enterobacteria and in other gram negative rods. Gentamicin resistance, and resistance to other aminoglycosides, is often due to the expression of a variety of modifying enzymes (AME, i.e., aminoglycoside modifying enzymes: acetylases, phosphorylases, and adenylases), which can impair the effectiveness of antibiotics (Shaw et al 1993).…”

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confidence: 80%

“…Both strains were resistant to the antimicrobial agents ampicillin, cephalotin, gentamicin, kanamycin, chloramphenicol, tellurite, displayed intermediary resistance to amicacin and, were sensitive to tetracyclin. E. coli HB101 harboring a pUC18 derivative plasmid containing gentamicin resistance gene (denominated pUJ401F10) originated from the plasmid pKP401F10 of K. pneumoniae (original strain isolated from faeces of a patient with urinary infection at HUPE/ UERJ) (Pereira 1990). Distinct clones of E. coli K12 F -transconjugant strains (1/1, 1/15 and 1/18) derived from the mating of the K. pneumoniae AS15-G6C strain with the E. coli K12 reference strain, containing multiresistance codifying plasmids, including gentamicin resistance.…”

Section: Methodsmentioning

confidence: 99%

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Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Barros

Pinheiro

Bozza

et al. 1999

Mem. Inst. Oswaldo Cruz

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The intestinal microbiota, a barrier to the establishment of pathogenic bacteria, is also an impor using as a probe a labeled cloned insert containing aminoglycoside modifying enzyme (AME) gene sequence originated from a plasmid of a Klebsiella pneumoniae strain previously isolated in the same hospital. Further, we found indications of inactivation to other resistance genes in variants selected under similar conditions, as well as, indications of co-amplification of other AME markers (amikacin).Since the intestinal environment is a scenario of selective processes due to the therapeutic and prophylactic use of antimicrobial agents, the processes of amplification of low level antimicrobial resistance (not usually detected or sought by common methods used for antibiotic resistance surveillance) might compromise the effectiveness of antibiotic chemotherapy.

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confidence: 80%

Section: Methodsmentioning

confidence: 99%

Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Barros

Pinheiro

Bozza

et al. 1999

Mem. Inst. Oswaldo Cruz

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“…Groups of genes (and the markers for resistance to antimicrobials) would by this hypothesis appear in a random manner. But, in environments with active selection by antimicrobials, the associations among genes (and subsequently among phenotypic markers for resistance) would occur in a statistically dependent manner, thus increasing the association frequencies of resistance genes (and of phenotypic markers), which in the context of random associations, would be relatively rare (25,27,28). Among the strains obtained in our study, those isolated from the hospital drain are examples of associations of resistance markers that are indicators of strong selection.…”

Section: Discussionmentioning

confidence: 99%

Ecological aspects of the antimicrobial resistence in bacteria of importance to humn infections

Meirelles-Pereira

Pereira

Silva

et al. 2002

Braz. J. Microbiol.

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In view of the intimate relationship of humans with coastal lagoons (used for recreation, tourism, water supply, etc.), the discharge of domestic effluents may lead to the establishment of routes of dissemination of pathogenic microorganisms, including microorganisms carrying genes for resistance to antimicrobials, through the surrounding human communities. The objective of the present investigation was to relate the presence of antimicrobial-resistant bacteria to the environmental characteristics of three coastal lagoons, comparing the results with those from hospital sewage. Of the lagoons evaluated, two (Geribá and Imboassica) receive domestic sewage discharge, and the other (Cabiúnas) is still in a natural state. We isolated in a culture medium containing 32 ¼ µg/ml of Cephalothin, fecal coliforms (E. coli), non-fecal coliforms (Klebsiella, Enterobacter, Serratia, and Citrobacter), non-glucose-fermenting Gram-negative bacilli, and Aeromonas sp. In cultures from the hospital drain we found strains showing numerous markers for resistance to most of the 11 antimicrobials tested. On the other hand, in cultures from Cabiúnas and Imboassica lagoons, we found strains showing resistance only to antibiotics frequently observed in non-selective situations (considered as "common" markers). The capacity for dilution in the ecosystem, and salinity appeared related with the occurrence of multi-resistant bacterial strains. The intensity of recent fecal contamination was not shown to be associated with the numbers and types of markers found.

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“…In addition to conferring resistance to oxidative stress on these strains, PT resistance may be associated (as a marker) with genes that are disseminated through plasmids carrying genes encoding antimicrobial resistance (Pereira et al, 1993;Santos et al, 2018). The associations between antimicrobial resistance genes, PT resistance (associated with resistance to phagocytosis and other oxidant processes) and the presence of IS26, may also be of importance for understanding the pathogenesis of Health Care Related Infections (HAI).…”

Section: Introductionmentioning

confidence: 99%

Dissemination of antimicrobial resistance and Potassium Tellurite (PT) in the hospital environment: can the use of means with Tellurite and the detection of insertion sequences contribute to prevention and intervention?

Fonseca¹,

Gonçalves²,

Souza³

et al. 2022

SHS

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Multidrug resistance is a public health problem. Enterobacteriaceae strains may be reservoirs of antimicrobial and potassium tellurite (PT) resistance genes, which is a determinant of bacterial escape to intraphagocytic killing mechanisms. The mobility of resistance genes and their genetic rearrangements are significantly involved in the dissemination of these genes. Mobile genetic elements such as integrons and transposons, as well as IS26 (considered among the predominant ISs), facilitate the formation and mobilization of sets of resistance genes in plasmids capable of transferring these determinants of resistance and virulence horizontally. There is evidence of associations, in plasmids, of genes encoding adhesins, toxins and antimicrobial resistance, possibly related to PT resistance. In the present work, 342 samples were collected for bacteriological culture in different units of a University Hospital in Rio de Janeiro, Brazil (Hospital Kitchen, Medical and Surgical Clinics Wards, and Intensive Care Unit): 98 samples from hands of professionals, 85 hand samples from food handlers, 104 stool samples (rectal swab) from patients and 55 food samples. We isolated 27 strains of Enterobacteriaceae through selective media containing cephalothin (32µg/mL). Bacterial identification was performed by conventional phenotypic methods. Antimicrobial susceptibility tests, as well as the phenotypic test for Extended-Spectrum Beta-lactamases (ESBLs) production, were performed by disk diffusion. PT resistance was also tested by direct seeding in media with 25µg/mL and 112 µg/mL of the compound. Plasmids were visualized by agarose gel electrophoresis of bacterial DNA extracts. Additionally, antimicrobial resistance genes and IS26 were detected by PCR assays. From a total of 27 strains of Enterobacteriaceae isolated, eight strains of Enterobacteriaceae were selected, considering phenotypes indicative of ESBLs production, resistance to aminoglycosides and presence of plasmids of high and low molecular weight (from the hands of food handlers, food and feces from hospitalized patients). Three strains 3/8 (37.5%) were resistant to aminoglycosides and cephalosporins, concomitantly. Four 4/8 strains (50.0%) were positive for the phenotypic test for ESBL production and six 6/8 strains (75.0%) were resistant to PT. Strains with resistance to aminoglycosides and producing ESBL showed amplification product for the genes tested. We consider that these antimicrobial resistance genes, also the determinants for resistance to PT (as a bacterial virulence factor), as well as IS26, may contribute to the plasticity and fitness of R plasmids, in different environments, favoring the circulation of relevant pathogenic strains.

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Estudo epidemiológico da infecção hospitalar e resistência a antimicrobianos e ao telurito em Enterobacteriaceae com referência especial a Klebsiella pneumonia

Cited by 3 publications

References 0 publications

Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Ecological aspects of the antimicrobial resistence in bacteria of importance to humn infections

Dissemination of antimicrobial resistance and Potassium Tellurite (PT) in the hospital environment: can the use of means with Tellurite and the detection of insertion sequences contribute to prevention and intervention?

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