Infecção experimental de macacos cebus apella sp pelo Trypanosoma cruzi: avaliação clínica, eletrocardiqgráfica e anatomopatológica

Abstract

Thirty-two monkeys were captured and adapted to laboratory conditions captives isolated. They were submitted to multiple xenodiagnosis which were negative. Twelve were infected intraperitoneally with different strains of T. cruzi (1.10(5) to 5.10(6)). Twenty were the control group. Between on to six years both the control group and the infected monkeys, were submitted to xenodiagnosis, serological testing clinical examination and electrocardiography. The clinical examination and the electrocardiogram were always normal. The monkey were autopsied and histological examination detected in the infected group four monkeys with evidence of disease: one with parasites in the tissue and three with chronic myocarditis. Parasitaemia was in 66.66% of the monkeys in the acute phase and the serology was positive in 91.66% in the chronic phase. The authors concluded that Cebus monkeys were not susceptible to the development of the disease but they could be utilized to maintain of such strains and studies of serological research in long-terms infections.

First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay

“…apella is highly susceptible to T. cruzi infection and has been used as a model for experimental infections, being able to maintain parasitemia during long-term infection, with low level of severe symptoms that characterized Chagas disease in humans and without mortality (ALMEIDA et al, 1992;RIARTE et al, 1995). Long-lasting natural infection was also observed in L. rosalia (golden lion Tamarin) specimens, which after ten-year follow-up were able to maintain high parasitemias (LISBOA et al, 2015).…”

Advances in Imaging of Animal Models of Chagas Disease

“…Non-human primates have also been explored as models for Chagas disease (Bonecini-Almeida Mda et al, 1990; de Almeida et al, 1992; de Meirelles Mde et al, 1990; Milei et al, 1982). Early studies revealed that ECG changes in infected monkeys were correlated with specific anatomic lesions (Milei et al, 1982).…”

“…In later studies, Cebus apella sp. monkeys were infected with different strains of T. cruzi and were submitted to xenodiagnosis, serological testing clinical examination and electrocardiography (de Almeida et al, 1992). The ECGs were always normal for the 12 infected monkeys in the study, and at autopsy, only three of the monkeys exhibited chronic myocarditis.…”

“…Interestingly, similar arguments have recently appeared in the literature questioning the relevance of the mouse model of cerebral malaria to human cerebral malaria (Taylor-Robinson, 2010). Thus, several studies have employed T. cruzi -infected larger animals such as rabbits, rats, guinea pigs, dogs and subhuman primates (Barbabosa-Pliego et al, 2009; Bonecini-Almeida Mda et al, 1990; Chandrasekar et al, 1998; Chen et al, 1996; Cruz-Chan et al, 2009; da Silva et al, 1996; de Almeida et al, 1992; de Meirelles Mde et al, 1990; Figueiredo et al, 1986; Guedes et al, 2002, 2009; Junqueira Junior et al, 1992; Labrador-Hernandez et al, 2008; Milei et al, 1982; Morris et al, 1991; Perez et al, 2009; Ramirez and Brener, 1987; Teixeira et al, 1983; Zabalgoitia et al, 2004). …”

Trypanosoma cruzi infection in Leontopithecus rosalia at the Reserva Biológica de Poço das Antas, Rio de Janeiro, Brazil

“…sciureus (Aben-Athar 1922, Chagas 1924, Deane & Damasceno 1961, Marinkelle 1966; Callithrix jacchus (Deane 1962); Chiropotes satanas, Pithecia pithecia, Callicebus torquatus (Deane et al 1970); Lagothrix lagotricha, Ateles fusciceps, Cebuella pygmaea, Saguinus mystax and S. oedipus (Ayala et al 1973, Marinkelle 1982. Although experimentally infected non-human primates develop an acute infection that is similar to acute Chagas disease, they do not develop a comparable chronic phase of the infection (Marsden et al 1970, Rosner et al 1988, Bonecini-Almeida et al 1990, Almeida et al 1992.…”