As técnicas de imunoperoxidase no estudo da etiologia das doenças infectuosas e parasitárias

Barbosa, Alfredo J. A.

doi:10.1590/s0037-86821988000100001

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…On the other hand, when using both LPG antibodies, the background reactions were higher. This may occur due to endogenous enzyme activity, inadequate fixation, sample degradation, or the presence of contaminating natural antibodies and proteins [8], [39]. The polymer-based technology produced results identical to the IHC method, with the advantage of being faster and less laborious.…”

Section: Discussionmentioning

confidence: 99%

American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin

et al. 2013

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BackgroundAmerican tegumentary leishmaniasis (ATL) is endemic in Latin America, where Brazil has over 27 thousand cases per year. The aim of the present study was to develop an immunohistochemical method (IHC) for ATL diagnosis. For this purpose, we used serum from a dog naturally infected with Leishmania (L) infantum (canine hyperimmune serum) as the primary antibody, followed by a detection system with a secondary biotinylated antibody.MethodologySkin samples were obtained from 73 patients in an endemic area of Caratinga, Minas Gerais (MG) State, Brazil all testing positive for ATL with the Montenegro skin test, microscopy, and PCR. Canine hyperimmune serum of a dog naturally infected with Leishmania (L.) infantum was employed as a primary antibody in an immunohistochemical diagnostic method using streptavidin-biotin peroxidase. To assess the specificity of this reaction, IHC assays employing two monoclonal antibodies were carried out. As the polymer-based technology is less time-consuming and labor intensive than the IHC labeled streptavidin-biotin peroxidase method, we compared the two methods for all samples.ResultsThe IHC method detected ATL in 67 of the 73 cases (91.8%). Immunolabeled parasites were primarily detected inside macrophages either in the superficial or the deep dermis. Detection was facilitated by the high contrast staining of amastigotes (dark brown) against the light blue background. A lower detection rate (71.2%) was observed with the both of the monoclonal Leishmania antibodies compared to the canine hyperimmune serum. This may have been due to a non-specific background staining observed in all histological samples rendering positive detection more difficult. The higher efficacy of the canine hyperimmune serum in the IHC method was confirmed by the method using streptavidin-biotin peroxidase as well as that with the polymer-based technology (biotin-avidin-free system).ConclusionsThe data are encouraging with regard to validating IHC as a standard alternative method for ATL diagnosis.

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Section: Discussionmentioning

confidence: 99%

American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin

et al. 2013

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“…There are direct techniques to identificate tachyzoites and cysts in animal tissues by means of: histological sections stained with hematoxylin and eosin (HE), immunohistochemistry (IHC) techniques and bioassays in mice (ROSA et al, 2001). In histological sections stained with HE, it is difficult to identify cysts of T. gondii because the parasite can be confused with nuclei or nuclear fragments that stain similarly (TSUNEMATSU et al, 1964;BARBOSA, 1988). This technique is generally used to look for histological lesions consistent with infection by this parasite, with or without associated use of immunohistochemistry (UGGLA et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Nunes

Silva²,

Oliveira³

et al. 2015

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate occurrence of Toxoplasma gondii in sheep slaughtered in the state of Alagoas, Brazil, by means of different diagnosis techniques. Serum samples and tissues from 100 slaughtered sheep were used. To detect antibodies, the indirect immunofluorescence antibody test (IFAT) was used, and tissues from seropositive animals (cut-off ≥1:64) were submitted to Polymerase Chain Reaction (PCR) and immunohistochemistry (IHC). To assess the concordance between the direct techniques, the kappa test was used. In the IFAT, it was observed that 14% (14/100) of the ovine samples were serum-positive. In the PCR, 21.43% (3/14) of the animals were positive and in IHC, it was observed that 7.14% (1/14) were positively stained for T. gondii in cerebral tissue. Histopathologically, the predominant finding was the presence of mononuclear cell infiltrate in the heart and a perivascular cuff in the cerebrum and cerebellum. The concordance between the direct diagnosis techniques was moderate (k=0.44). Thus, it is important to use different direct techniques in diagnosing toxoplasmosis in naturally infected sheep.

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“…Já o exame histopatológico é específico, porém não é uma técnica considerada conclusiva para o diagnóstico da infecção por T. gondii, visto que o mesmo pode ser confundido com núcleos ou fragmentos nucleares corados, de forma semelhante, por causa da ausência de características tintoriais próprias (TSUNEMATSU et al, 1964;BARBOSA, 1988).…”

Section: Conclusãounclassified

Toxoplasmose Ovina: Revisão de Literatura

Lima

Melo²

2022

UNICIÊNCIAS

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A ovinocultura é desenvolvida em diversas regiões do país e do mundo, formada por animais com capacidade de adaptação a condições ambientais adversas, e neste cenário, o Brasil se destaca por ser considerado o primeiro produtor de ovinos do Continente Americano, com um rebanho de aproximadamente 18 milhões de cabeças. Nesse contexto, diversas enfermidades infecciosas têm provocado grandes prejuízos, incluindo aquela causada pelo protozoário Toxoplasma gondii, que resulta em problemas reprodutivos e perdas econômicas na ovinocultura. O T. gondii é o agente etiológico da toxoplasmose, uma importante zoonose, com distribuição cosmopolita e de grande interesse em saúde pública. Trata-se de um agente que pode provocar sérios danos aos fetos, pela transmissão vertical tanto em humanos quanto nos animais podendo causar abortamento ou infecção congênita em seus hospedeiros intermediários, tendo os felídeos como os únicos hospedeiros definitivos. Este artigo foi elaborado por meio de pesquisa bibliográfica, com o objetivo de abordar acerca da toxoplasmose ovina. Diversos trabalhos têm demonstrado a detecção do parasito em carnes destinadas ao abate, sendo muito relevante em âmbito de saúde pública, pois é uma fonte de infecção para os humanos caso não seja realizado o cozimento destes alimentos de maneira eficiente. Por isso, é essencial que sejam estabelecidas medidas sanitárias nas criações de ovinos como forma de prevenir a propagação da doença. Palavras-chave: Protozoário. Ovinocultura. Abortamento. Abstract Sheep farming is developed in several regions of the country and the world, made up of animals with the ability to adapt to adverse environmental conditions, and in this scenario, Brazil stands out for being considered the first sheep producer on the American continent, with a herd of approximately 18 million heads. In this context, several infectious diseases have caused great damage, including that caused by the protozoan Toxoplasma gondii, which results in reproductive problems and significant economic losses in sheep farming. T. gondii is the etiologic agent of toxoplasmosis, an important zoonosis, with cosmopolitan distribution and great interest in public health. It is an agent that can cause serious damage to fetuses, due to vertical transmission in both humans and animals, causing abortion or congenital infection in their intermediate hosts, and the felids are the only definitive hosts. This article was elaborated through bibliographical research, with the objective of approaching the one about sheep toxoplasmosis. Several studies have demonstrated the parasite detection in meat intended for slaughter, which is very relevant in terms of public health, as it is a source of infection for humans if these foods are not cooked efficiently. Therefore, it is essential to establish sanitary measures in sheep farming as a way to prevent the spread of the disease. Keywords: Protozoa. Sheep farming. Abortion.

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As técnicas de imunoperoxidase no estudo da etiologia das doenças infectuosas e parasitárias

Cited by 4 publications

References 15 publications

American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin

American Tegumentary Leishmaniasis: Effectiveness of an Immunohistochemical Protocol for the Detection of Leishmania in Skin

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Toxoplasmose Ovina: Revisão de Literatura

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