Efeito da dessecação das fezes de triatomíneos na sobrevivência de formas metacíclicas de Trypanosoma cruzi

Soares, V.A.; Marsden, Philip D.; Johnson, C. E.

doi:10.1590/s0037-86821986000400006

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…It is important to point it out that the detection of T. cruzi DNA cannot be directly associated with the oral transmission of Chagas disease by the consumption of açai as DNA can also be detected from recently inactivated parasites. Nevertheless, previous studies showed that, at high humidity, T. cruzi in triatomine faeces preserves their mobility and infectivity up to 30 min at 33 °C [ 34 ]. Furthermore, T. cruzi can remain infective inside dead triatomines stored at 10 °C for six days and between 26 and 30 °C for at least two months [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil

et al. 2018

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BackgroundSeveral cases of food-borne acute Chagas disease (ACD) have been reported in the Brazilian Amazon so far. Up to 2004, the occurrence of ACD by oral transmission, associated with food consumption, was rare. Recent cases of ACD in Brazil have been attributed to the consumption of juice from the açai palm containing reservoir animals or insect vectors waste, infected with Trypanosoma cruzi. This study aimed to determine the T. cruzi contamination rate and to genotype the parasite in food samples prepared from açai, which are commercialized in Rio de Janeiro and the Pará States in Brazil.MethodsThe amplificability of DNA extracted from açai samples, and T. cruzi and Triatominae detection were performed by conventional PCR. Molecular characterization was done by multilocus PCR analysis, to determine the parasite discrete type units (DTUs) based on the size of PCR products in agarose gels, using the intergenic region of the spliced leader (SL), 24 Sα rDNA and nuclear fragment A10 as targets.ResultsFrom the 140 samples of açai-based products analyzed, T. cruzi DNA was detected in 14 samples (10%); triatomine DNA was detected in one of these 14 samples. The parasite genotyping demonstrated that food samples containing açai showed a mixture of T. cruzi DTUs with TcIII, TcV and TcI prevailing.ConclusionsIn this study, the molecular detection and identification of T. cruzi from açai-based manufactured food samples, was performed for the first time. Although parasite DNA is a marker of possible contamination during food manufacturing, our findings do not indicate that açai is a source of Chagas disease via oral transmission per se, as live parasites were not investigated. Nevertheless, a molecular approach could be a powerful tool in the epidemiological investigation of outbreaks, supporting previous evidence that açai-based food can be contaminated with T. cruzi. Furthermore, both food quality control and assessment of good manufacturing practices involving açai-based products can be improved, assuring the safety of açai products.

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Section: Discussionmentioning

confidence: 99%

Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil

et al. 2018

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Transmission of Chagas Disease (American Trypanosomiasis) by Food

Pereira

Schmidt

Barbosa

et al. 2010

Advances in Food and Nutrition Research

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Notas sobre o Trypanosoma cruzi e suas características bio-ecológicas, como agente de enfermidades transmitidas por alimentos

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2006

Rev. Soc. Bras. Med. Trop.

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A transmissão do Trypanosoma cruzi por via oral (VO) tem caráter habitual no ciclo enzoótico primitivo deste parasita, através da ingestão -por mamíferos suscetíveis -de vetores e reservatórios infectados. No caso do homem, esta transmissão ocorre de maneira esporádica e circunstancial, através de alimentos contaminados com o parasita, principalmente a partir de triatomíneos ou de suas dejeções 13 25 60 . Também, pode ocorrer através da ingestão de carne crua ou mal cozida de caça, ou de alimentos contaminados por urina ou secreção anal de marsupiais infectados, por acidentes em laboratório ou, mesmo, por meio de hábitos primitivos de ingestão de triatomíneos 6 19 26 56 . A presente nota objetiva resgatar as principais informações registradas sobre a transmissão do Trypanosoma cruzi por VO, como subsídio aos esforços para seu melhor conhecimento e prevenção, buscando-se resumir a experiência acumulada e ressaltar alguns aspectos do parasita, mais pertinentes esta modalidade de transmissão. Como pressuposto, admite-se que, no ciclo silvestre, a transmissão oral seguirá sendo uma forma habitual e freqüente de circulação do parasita, independentemente das ações de controle do vetor domiciliado e da seleção de doadores infectados nos bancos de sangue 17 55 58 . Notas sobre o Trypanosoma cruzi e suas características bio-ecológicas, como agente de enfermidades transmitidas por alimentos ABSTRACTExperimental evidence and field observations concerning the oral transmission of Trypanosoma cruzi to human beings and mammalian species are reviewed, confirming its unquestionable occurrence, mainly in the enzootic cycle of the parasite. Several types of foods and carriers of the flagellate have been involved in this transmission route, where the human cases are generally related to infected triatomines and/or natural reservoirs in the proximities of the event. The penetration points of the parasite (mouth, esophagus, stomach and intestinal mucosae) are reviewed, as well as the general anatomic and pathological processes, such as hemorrhagic phenomena, mesenteritis and interstitial hepatitis. In particular, different aspects of the biology and biochemistry of the parasite are analyzed, especially in terms of its epidemiology and the possibilities of the prevention of the oral transmission of Chagas' disease.

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Efeito da dessecação das fezes de triatomíneos na sobrevivência de formas metacíclicas de Trypanosoma cruzi

Cited by 3 publications

References 2 publications

Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil

Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil

Transmission of Chagas Disease (American Trypanosomiasis) by Food

Notas sobre o Trypanosoma cruzi e suas características bio-ecológicas, como agente de enfermidades transmitidas por alimentos

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