Capillaria hepatica: alguns aspectos imunopatológicos da infecção espúria e da infecção verdadeira

Nascimento, Ivana; Sadigursky, Moysés

doi:10.1590/s0037-86821986000100005

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…Besides, positive fluorescence was never observed in control sections or in cases of C. hepatica spurius infection (animals subjected to ingestion of non-infective immature eggs). Spurious infection has been claimed to sensitize the host on the basis of the ELISA test (Nascimento & Sadigursky 1986), a finding not confirmed in the present investigation, especially when the IIF was applied. Fluorescence was then weak and non-specific, disappearing when dilutions of 1:400 or over were employed.…”

Section: Discussioncontrasting

confidence: 80%

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A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test

Assis

Cunha

Baptista

et al. 2004

Mem. Inst. Oswaldo Cruz

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A highly specific pattern of immunofluorescence was noted when sera from Capillaria hepatica-infected rats were tested against the homologous worms and eggs present either in paraffin or cryostat sections from mouse liver. The pattern was represented by a combined apple green fluorescence of the internal contents of worms and eggs, which persisted in serum-dilutions of 1:400 up to 1:1600. Unequivocal fluorescent pattern was observed from 15 days up to 3 months following inoculation of rats with embryonated C. hepatica eggs and such result was confirmed by the ELISA. After the 4th month of infection, the indirect immunofluorescence test turned negative, probably revealing the extinction of parasitism, however the ELISA was contradictory, disclosing high levels of antibodies in this period. The IIF was also negative when control normal rat sera and sera from rats administered by gavage with immature C. hepatica eggs (spurious infection), or for reactions made against Schistosoma mansoni eggs, although a weakly positive pattern occurred with Fasciola hepatica eggs. The indirect immunofluorescence test may be recommended for use with human sera to detect early C. hepatica infection in special clinical instances and in epidemiological surveys, since it is a simple, inexpensive, and reliable test, presenting excellent sensitivity and specificity. Although the diagnosis is positive only during early infection, this is the period when the symptoms are usually more severe and the need for differential diagnosis is greater.

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Section: Discussioncontrasting

confidence: 80%

“…Group 3 -Ten rats were administered with 600 immature eggs, representing a so-called spurious infection (Nascimento & Sadigursky 1986).…”

Section: Methodsmentioning

confidence: 99%

A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test

Assis

Cunha

Baptista

et al. 2004

Mem. Inst. Oswaldo Cruz

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“…During natural infection they are retained within the liver for prolonged periods of time, but once in the external environment, with appropriate conditions of moisture and temperature, they are able to mature and infect. In cases of so-called spurius infection , when immature eggs are ingested by a susceptible host, they pass unchanged through the gastrointestinal tract, but are able to immunologically sensitize the host 8 . Their chitinous shell is difficult to digest once inside the host tissues, so it may retard resorption of the lesions and act as adjuvant for host sensitization.…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of hepatic septal fibrosis associated with Capillaria hepatica infection of rats

Santos¹,

Tolentino²,

Andrade

2001

Rev. Soc. Bras. Med. Trop.

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Septal fibrosis is a common form of hepatic fibrosis, but its etiology and pathogenesis are poorly understood. Rats infected with the helminth Capillaria hepatica constitute a good experimental model of such fibrosis. To investigate the pathogenetic contribution of the several parasitic factors involved, the following procedures were performed in rats: a) regarding the role of eggs, these were isolated and injected either into the peritoneal cavity or directly into the liver parenchyma; b) for worms alone, 15-day-old infection was treated with mebendazole, killing the parasites before oviposition started; c) for both eggs and worms, rats at the 30th day of infection were treated with either mebendazole or ivermectin. Eggs only originated focal fibrosis from cicatricial granulomas, but no septal fibrosis. Worms alone induced a mild degree of perifocal septal fibrosis. Systematized septal fibrosis of the liver, similar to that observed in the infected controls, occurred only in the rats treated with mebendazole or ivermectin, with dead worms and immature eggs in their livers. Thus, future search for fibrogenic factors associated with C. hepatica infection in rats should consider lesions with both eggs and worms.

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“…Although previously published studies show that the prevalence of C. hepatica in certain rodent species, tends to be high (between 56.5 and 89.3%) 5 , 7 , 20 , 31 , the prevalence of C. hepatica infection appears to be low among residents of the studied area and among rodents that live close to their dwellings.…”

Section: Discussionmentioning

confidence: 57%

STUDY OF THE PREVALENCE OF Capillaria hepatica IN HUMANS AND RODENTS IN AN URBAN AREA OF THE CITY OF PORTO VELHO, RONDÔNIA, BRAZIL

Rocha

Basano

Souza

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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Introduction: Hepatic capillariosis, caused by Capillaria hepatica (Calodium hepaticum) (Bancroft, 1893), Travassos, 1915 (Nematoda, Trichinelloidea, Capillariidae), is a common zoonosis in rodents but is rare in humans. Seventy-two cases in humans have been reported worldwide since the first case was described by MACARTHUR in 192417,27. This study aimed to determine the prevalence of Capillaria hepatica in humans and rodents in an urban area of Porto Velho, the capital of Rondônia, in Brazil. Methods: After conducting a census of the area, 490 residents were randomly selected, and, after signing a term of consent, provided blood samples that were screened for anti-Capillaria hepatica antibodies. Simultaneously, rats were captured to assess the prevalence of this parasite in rodents by histopathological examination in liver sections. Results: A prevalence of 1.8% was found among residents who had specific antibodies at a dilution of 1:150, indicating exposure to parasite eggs; 0.8% of the subjects also had positive titers at a dilution of 1:400, indicating true infection. The prevalence in rats was 2%. Conclusions: The prevalence of infection with this parasite among humans and rats was low. While the prevalence encountered among humans was within the limits reported in the literature, the prevalence among rodents was much lower.

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Capillaria hepatica: alguns aspectos imunopatológicos da infecção espúria e da infecção verdadeira

Cited by 3 publications

References 14 publications

A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test

A contribution to the diagnosis of Capillaria hepatica infection by indirect immunofluorescence test

Pathogenesis of hepatic septal fibrosis associated with Capillaria hepatica infection of rats

STUDY OF THE PREVALENCE OF Capillaria hepatica IN HUMANS AND RODENTS IN AN URBAN AREA OF THE CITY OF PORTO VELHO, RONDÔNIA, BRAZIL

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