Exames de laboratório: sensibilidade, especificidade, valor preditivo positivo

Guimarães, Morgana Rodrigues

doi:10.1590/s0037-86821985000200009

Cited by 8 publications

(6 citation statements)

References 1 publication

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“…In previous studies, the DPP ® test showed specificity ranging from 87.8% to 98.6% and sensitivity between 90.6% and 98% using confirmed positive samples 9,10 . However, despite showing high levels of sensitivity among clinically symptomatic dogs, sensitivity of the DPP ® test to identify the Leishmania infection in asymptomatic dogs was only 47% in one of the studies 13 . In a systematic review and a meta-analysis of the data from 25 studies, Peixoto et al 20 concluded that the ELISA tests using crude antigens and the DPP ® tests have moderate accuracy (83% [ 95% CI: 78%-88%] sensitivity and 73% [ 95% CI: 70%-75%] specificity).…”

Section: Discussionmentioning

confidence: 94%

“…The results of the two tests were compared using agreement analysis to establish the serological parameters of sensitivity and specificity, and the observed and expected agreement indicators [13][14][15] . The accuracy was expressed by the kappa (κ) index by inferring more rigor in relation to general agreement indicators, taking into account the proportions of the expected and observed agreement.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Souza

Silva

Labarthe

2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Visceral leishmaniasis is a disease that affects humans, wildlife, and domestic species. Since dogs play a key role in urban Leishmania spp. transmission, the Brazilian government maintains the Monitoring and Control Program of Visceral Leishmaniasis (VLMCP) in endemic regions, which promotes awareness campaigns aiming to enhance the control of the infection. The VLMCP recommends the Dual Path Platform (DPP ® ) canine visceral leishmaniasis test (Bio-Manguinhos, Brazil) for screening and enzyme-linked immunosorbent assay to confirm the infection. The DPP ® test is produced and distributed by the Health Ministry to the Municipal Health Centers responsible for the local VLMCP. The test is not available to all the clinics, forcing some veterinarians to use other rapid tests for screening and diagnosis of this disease in their daily routine. Methods: The present study was conducted to compare the performance of the DPP ® and SNAP ® tests using sera from the dogs with confirmed infections of L. infantum and from the dogs with no previous testing, residing in areas with a low Leishmania infection. Results: There was 97.0% agreement between the two tests. Sensitivity and specificity of the SNAP ® test were 96.3% and 100%, respectively. Agreement between both the antibody tests and the parasitological detection methods was 96.8%. The DPP ® test had 95.8% sensitivity and 100% specificity. Conclusions: The SNAP ® and the DPP ® tests were virtually equivalent in terms of detection of canine antibodies against L. infantum, and both the tests demonstrated high and similar levels of sensitivity and specificity.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Souza

Silva

Labarthe

2019

Rev. Soc. Bras. Med. Trop.

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“…A partir disso, definiu-se a concentração de 0.2µM como a mais adequada para uma amplificação eficiente, sem excesso de primers, o que poderia ocasionar a formação de dímeros e anelamentos inespecíficos (BUSTIN et al, 2009 Guimarães (1985), para avaliar o desempenho da qPCR frente ao cultivo microbiológico.…”

Section: Reação Em Cadeia Da Polimerase Em Tempo Real (Qpcr)unclassified

Desenvolvimento de primers para identificação e diferenciação de espécies de Candida em secreção vaginal por PCR em tempo real (qPCR)

Jackisch

Koehler

Toillier

et al. 2017

RJP

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RESUMOInfecções fúngicas são cada vez mais frequentes, principalmente relacionadas ao sistema geniturinário e causadas por Candida spp. A PCR mostra-se promissora para o diagnóstico e para sua aplicação faz-se necessário o desenvolvimento de primers. Para isso obteve-se sequências da região ITS do rDNA 18S de 5 espécies de Candida, alinhou-se e verificou-se os tamanhos dos amplicons e as Tms teóricas. Cepas padrão e cepas cultivadas foram utilizadas como referência. Além disso, 67 amostras de DNA de secreção vaginal foram submetidas a qPCR e os resultados foram confirmados em gel de agarose 2%. Os testes in silico demonstraram a capacidade do par de primers em diferenciar as cinco espécies, o que foi confirmado após a amplificação das cepas padrão e de amostras de secreção vaginal, identificadas em 20 amostras. O limite de detecção foi de 58 células/mL para as cepas padrão de C. albicans e C. tropicalis e 29 células/mL para C. parapsilosis. A sensibilidade da qPCR frente ao teste de crescimento microbiológico foi de 68%, com especificidade de 90%. A técnica possui potencial de diagnóstico, porém precisa ser otimizada para testes quantitativos.Palavras-chave: Infecção geniturinária. qPCR. Candida spp. Desing de primers. ABSTRACTFungal infections are increasingly frequent, mainly related to the genitourinary system and caused by Candida spp. The PCR is promising for the diagnosis and for its application it is necessary the development of primers. For this purpose 18S rDNA ITS region sequences were obtained from 5 Candida species, aligned and the sizes of the amplicons and the theoretical Tms were verified. Standard and cultivated strains were used as reference. In addition, 67 samples of vaginal secretion DNA were subjected to qPCR and the results were confirmed on 2% agarose gel. In silico tests demonstrated the ability of the pair of primers to differentiate the five species, which was confirmed after amplification of standard samples and vaginal secretion (it was possible to identify the species in 20 samples). The detection limit was 58 cells/mL for the standard C. albicans and C. tropicalis strains and 29 cells/mL for C. parapsilosis. The sensitivity of qPCR to the microbiological growth test was 68%, with specificity of 90%. The technique has diagnostic potential, but needs to be optimized for quantitative tests.

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“…Para efeito de cálculo da sensibilidade e especificidade do Dot--ELISA, utilizaram-se as fórmulas propostas por Guimarães MCS (1985):…”

Section: Cálculo De Sensibilidade E Especificidadeunclassified

Desenvolvimento e padronização do Dot-ELISA usando peptídeos recombinantes para o diagnóstico sorológico de Neospora caninum

et al. 2014

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Pesq. Vet. Bras. 34(8):723-727, agosto 2014 723 RESUMO.-A neosporose é reconhecida como uma das maiores causas de aborto e perdas neonatais em bovinos de leite e corte em todo o mundo. Nos últimos anos esta doença tem atraído o interesse de pesquisadores com foco na epidemiologia e métodos eficazes de diagnóstico desta doença. No presente estudo objetivou-se desenvolver e pa- Neosporosis is recognized as a major cause of abortion and neonatal loss in cattle worldwide, both for dairy cattle and beef cattle. In recent years this disease has attracted the interest of researchers and studying the epidemiology and effective methods of diagnosis of this disease. The present study aimed to develop and standardize on a Dot-ELISA for the serological diagnosis of Neospora caninum by using recombinant peptide as antigen for the development of a diagnostic kit used on the field. The recombinant antigen (rNcGRA1) was designed based on the method of reverse genetics derived antigenic epitopes of dense granules protein of N. caninum and synthesized by GenScript (USA). It was produced by the fermentation in yeasts Pichiapastoris KM71. The serological technique was used for the Dot-ELISA detection of IgG specific for N. caninum in which 0.22μm nitrocellulose membranes were sensitized with 1μL of antigen and subsequently the plasmas were diluted in a washing solution and incubated for 1 hour. The results will revealed by the addition of Protein G labeled with peroxidasse for 30 minutes, followed by the developing solution based on 3,3'-Diaminobenzidine (DAB). Soon after standardization tested 44 bovine plasmas were diagnosed by indirect immunofluorescence assay (IFA), agreeing with the results on a 95.5% and a sensitivity and specificity of 100% and 92% respectively. In regard to the diagnostic kit for the Technology Platform Rapid Flow-Through Miriad ® , the peptide presented rNcGRA1 visible markings to react with positive plasma, and showed no markings using the negative plasma. This study is the first to use recombinant peptides and prove to be efficient for the serological diagnosis of cattle naturally infected.

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Exames de laboratório: sensibilidade, especificidade, valor preditivo positivo

Cited by 8 publications

References 1 publication

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Evaluation of DPP® and SNAP® Rapid Tests for diagnosis of Leishmania infantum canine infections

Desenvolvimento de primers para identificação e diferenciação de espécies de Candida em secreção vaginal por PCR em tempo real (qPCR)

Desenvolvimento e padronização do Dot-ELISA usando peptídeos recombinantes para o diagnóstico sorológico de Neospora caninum

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