Hemoculturas para o diagnóstico parasitológico da fase crônica da doença de Chagas

Mourão, Oto G.; Mello, Odeni C.

doi:10.1590/s0037-86821975000400004

Cited by 12 publications

(4 citation statements)

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“…Aliquots of 2 mL blood samples collected by venipuncture in sodium citrate tubes were added to bacto agar blood base culture medium and observed every 10 days. Once the cultures were found to be positive for epimastigotes, they were transferred to liver infusion tryptose medium with 10% fetal bovine serum ( Mourão & Mello, 1975 ).…”

Section: Methodsmentioning

confidence: 99%

Orally-transmitted Chagas disease: Epidemiological, clinical, serological and molecular outcomes of a school microepidemic in Chichiriviche de la Costa, Venezuela

Noya

Colmenares

Díaz-Bello

et al. 2016

Parasite Epidemiology and Control

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Oral transmission of Trypanosoma cruzi is a frequent cause of acute Chagas disease (ChD). In the present cross-sectional study, we report the epidemiological, clinical, serological and molecular outcomes of the second largest outbreak of oral ChD described in the literature. It occurred in March 2009 in Chichiriviche de la Costa, a rural seashore community at the central littoral in Venezuela. The vehicle was an artisanal guava juice prepared at the local school and Panstrongylus geniculatus was the vector involved. TcI genotype was isolated from patients and vector; some showed a mixture of haplotypes. Using molecular markers, parasitic loads were high. Eighty-nine cases were diagnosed, the majority (87.5%) in school children 6–15 years of age. Frequency of symptomatic patients was high (89.9%) with long-standing fever in 87.5%; 82.3% had pericardial effusion detected by echocardiogram and 41% had EKG abnormalities. Three children, a pregnant woman and her stillborn child died (5.6% mortality). The community was addressed by simultaneous determination of specific IgG and IgM, confirmed with indirect hemagglutination and lytic antibodies. Determination of IgG and IgA in saliva had low sensitivity. No individual parasitological or serological technique diagnosed 100% of cases. Culture and PCR detected T. cruzi in 95.5% of examined individuals. Based on the increasing incidence of oral acute cases of ChD, it appears that food is becoming one of the most important modes of transmission in the Amazon, Caribbean and Andes regions of America.

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Section: Methodsmentioning

confidence: 99%

Orally-transmitted Chagas disease: Epidemiological, clinical, serological and molecular outcomes of a school microepidemic in Chichiriviche de la Costa, Venezuela

Noya

Colmenares

Díaz-Bello

et al. 2016

Parasite Epidemiology and Control

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“…Once the cultures were found to be positive for epimastigotes, they were transferred to liver infusion tryptose medium with 10% foetal bovine serum (Mourão & Mello 1975).…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease

Noya

Díaz-Bello

Colmenares

et al. 2012

Mem. Inst. Oswaldo Cruz

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Orally transmitted Chagas disease (ChD), which is a well-known entity in the Brazilian Amazon Region, was first documented in Venezuela in December 2007, when In Venezuela, effective control measures successfully reduced the annual incidence of Trypanosoma cruzi infections from 10/1,000 individuals in the 1950s to 1/1,000 in the 1980s (Feliciangeli et al. 2003). Since then, the recorded outbreaks of acute Chagas disease (ChD) have been as follows: between 1988-1996, 59 cases occurred in the western states of Venezuela (Añez et al. 1999), after which nine cases involving two deaths occurred during the period of January 2006-March 2007 in the same endemic area (Añez et al. 2007). In 2004 and 2006, the deaths of two children near Caracas were documented ). Nevertheless, an outbreak of orally transmitted ChD (OChD) in a school in a middle class neighbourhood in Caracas was the warning signal that ChD was re-emerging via a different mode of transmission, i.e., the oral route (Alarcón de Noya et al. 2010b Noya et al. 2010b), an outbreak occurred in an urban school that was presumably linked to the ingestion of contaminated home-made juice that had been prepared in a different part of the city where triatomines were present.The aim of this study is to provide information about the parasitological, immunological and molecular laboratory tests used in diagnosing ChD to gleam further knowledge from the largest OChD outbreak ever reported. SUBJECTS, MATERIALS AND METHODSStudy population -The study population consisted of students, teachers and administrative personnel from a school located in the municipality of Chacao in Caracas, Venezuela. Once the primary case was diagnosed (Martín et al. 2009) and the epidemiological link to another case was established (Alarcón de Noya et al. 2010c), parasitological examinations and serological tests were carried out in these cases, in addition to other symptomatic patients who voluntarily requested medical attention at the Tropical Medicine Institute (IMT), Universidad Central de Venezuela in Caracas. After the death of a five-year-old student, most of the school community was managed as part of an on-site emergency, both in the school and at the hospital, where several of the patients were hospitalised. T. cruzi infection was diagnosed by collecting only one tube of blood for serological testing, except for in people who were symptomatic at the time of the interview and from whom additional blood was taken for parasitological or molecular testing (Supplementary data). For logistical and ethical reasons, a unique protocol could not be followed and, consequently, the results are simply presented as they were obtained over time. It was not possible to re-sample the blood of individuals with positive serological tests to isolate the parasite before treatment because the treatment had to begin before the group dispersed for the Christmas holiday.Direct parasitological tests -Observations of the parasite were performed using fresh blood smears as well as Giemsa-stained preparations (W...

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“…The hemoculture technique got credibility when CHIARI & BRENER, in 1966, got 31.8% of positivity using the LIT medium, bringing new possibilities 3 . MOURÃO & MELLO, in 1975, developed a process for removing plasma and washing the cells to remove the antibodies or other factors that could apparently inhibit growth of T. cruzi 7 .…”

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confidence: 99%

Trypanosoma cruzi parasitemia observed in immunocompromised patients: the importance of the artificial xenodiagnosis

Braz

Neto

Carignani

et al. 2001

Rev. Inst. Med. trop. S. Paulo

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Trypanosoma cruzi parasitemia observed in immunocompromised patients (transplant or positive HIV) occurred more frequently by the artificial xenodiagnosis method (10/38) compared with hemoculture (2/38), given the same quantity of blood. Other ways of diagnosis, like mice inoculation (5/38), QBC and buffy coat (2/38), were evaluated also. This result showed the importance of the artificial xenodiagnosis. The other techniques increased only one more patient positive.

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Hemoculturas para o diagnóstico parasitológico da fase crônica da doença de Chagas

Cited by 12 publications

References 1 publication

Orally-transmitted Chagas disease: Epidemiological, clinical, serological and molecular outcomes of a school microepidemic in Chichiriviche de la Costa, Venezuela

Orally-transmitted Chagas disease: Epidemiological, clinical, serological and molecular outcomes of a school microepidemic in Chichiriviche de la Costa, Venezuela

The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease

Trypanosoma cruzi parasitemia observed in immunocompromised patients: the importance of the artificial xenodiagnosis

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