Nota sobre uma nova técnica de hemocultura para diagnóstico parasitológico na doença de chagas na sua fase crônica

Chiari, Égler; Dias, João Carlos Pinto

doi:10.1590/s0037-86821975000300004

Cited by 22 publications

(20 citation statements)

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“…40 This same situation seems to occur with PCR performed in blood, where positive results in different chagasic populations and techniques fluctuate 45-100%. [20][21][22]41,42 The simultaneous application of 2 parasitologic tests increases diagnostic sensitivity, 43,44 but PCR is more sensitive in detecting circulating parasites. Our data indicate 74.5% agreement between positive PCR in blood and the presence of circulating parasites detected by indirect parasitologic tests, which is similar to findings in published data.…”

Section: Discussionmentioning

confidence: 99%

Relationship between Trypanosoma cruzi and human chagasic megaesophagus: blood and tissue parasitism.

Lages‐Silva

Crema²,

Ramı́rez³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. The persistence of Trypanosoma cruzi in tissue and blood of 52 patients in the digestive form of chronic Chagas disease was studied. These patients had chagasic megaesophagus and underwent corrective surgery. Parasitologic (xenodiagnosis, hemoculture, or both), histopathologic (hematoxylin and eosin, and peroxidase-anti-peroxidase staining), and molecular (polymerase chain reaction [PCR] followed by slot-blot hybridization) tests were used in the analysis. The presence of T. cruzi, its genomic fragments, or its antigens could be detected in 98% (51 of 52) of the patients. The parasite was randomly identified in 76.9% of esophageal tissues and in 90.4% by PCR and in 73.1% by parasitologic methods from the blood. Fifty percent (26 of 52) of tissue samples had inflammation, 80.8% of which was associated with the parasite. Trypanosoma cruzi was also identified unassociated with inflammatory alterations. Higher tissue parasitism and intense inflammatory processes were observed in esophageal tissue from patients with Grade IV megaesophagus. These data demonstrate that in the digestive form of Chagas' disease, particularly in cases of megaesophagus, T. cruzi is frequently found, both in blood and tissues and may contribute to the pathogenic mechanisms involved.

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Section: Discussionmentioning

confidence: 99%

Relationship between Trypanosoma cruzi and human chagasic megaesophagus: blood and tissue parasitism.

Lages‐Silva

Crema²,

Ramı́rez³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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“…It should be pointed out that prior to the development of this approach, the method used until for parasitological diagnosis of this phase was that first performed by Chagas (the wet blood smear and animal inoculation). For the chronic phase, during this period, parasitological diagnosis included hemoculture (Chiari & Brenner 1966). Serological diagnosis was firmly established with the standardization of the CFR [Almeida & Fife 1976, the introduction of indirect haemaglutination (IHA)] (Cerisola et al 1962), IgG-IIF indirect immunofluorescence (Camargo 1966) and the immunoenzymatic ELISA test (Voller et al 1975).…”

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confidence: 99%

Diagnosis of Chagas disease: what has been achieved? What remains to be done with regard to diagnosis and follow up studies?

Gomes

Lorena

Luquetti

2009

Mem. Inst. Oswaldo Cruz

118

107

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“…Embora os resultados apresentados na Tabela 2 tenham dem onstrado que não foi possível aum entar a sensibilidade do m é todo, através da utilização de um a 3.a sé rie de colheitas, a observação dos resulta dos obtidos por paciente (Tabela 1) assi nalou que a obtenção de hem oculturas po sitivas é aleatória, sendo no entanto um a decorrência da repetição dos exames (Ta bela 2), como já haviam adm itido Chiari & B rener (4). P ortanto a distribuição da freqüência de hem oculturas positivas por pacientes, por colheita ou por série pode apresentar variações entre diferentes ex perimentos, sendo aconselhável a m anu tenção de um esquema padronizado que possibil-te um a análise com parativa dos resultados obtidos.…”

Section: Discussionunclassified

Comprovação parasitológica na fase crônica da doença de Chagas por hemoculturas em meio "LIT"

Mourão¹,

Chiari

1975

Rev. Soc. Bras. Med. Trop.

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Foram realizadas hemoculturas seriadas, semeando-se 0,2 ml de sangue de pacientes com Doença de Chagas na fase crônica, em cada um de 14 tubos de ensaio contendo 6 ml de meio LIT. o exame foi repetido por 3 dias consecutivos, obtendo-se um total de 42 tubos semeados, correspondentes a uma série. A colheita do material foi repetida 30 e 60 dias após. As hemoculturas foram mantidas a 28°C e examinadas com 30, 45 e 60 dias. Dos 1337 tubos semeados 54 apresentaram-se positivos, observando-se que a maior freqüência de tubos positivos ocorreu após 45 dias de incubação, o que permitiu o diagnóstico parasitológico de 13 entre 15 pacientes examinados (86,6%). A hemocultura seriada em. meio LIT deve ser considerada como uma técnica a ser utilizada na triagem de pacientes para ensaios clínicos ou outros tipos de pesquisas que indiquem a necessidade de prévio diagnóstico parasitológico. Por ser um método trabalhoso e demorado não é possível recomendar o seu emprego em diagnóstico de rotina.

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Nota sobre uma nova técnica de hemocultura para diagnóstico parasitológico na doença de chagas na sua fase crônica

Cited by 22 publications

References 0 publications

Relationship between Trypanosoma cruzi and human chagasic megaesophagus: blood and tissue parasitism.

Relationship between Trypanosoma cruzi and human chagasic megaesophagus: blood and tissue parasitism.

Diagnosis of Chagas disease: what has been achieved? What remains to be done with regard to diagnosis and follow up studies?

Comprovação parasitológica na fase crônica da doença de Chagas por hemoculturas em meio "LIT"

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