Estudo dos caracteres de uma amostra boliviana de Trypanosoma cruzi

Biological characterization of Trypanosoma cruzi strains

“…cruzi strains -Five strains of T. cruzi from different biological and geographic origins were studied. Bolivia strain (Funayama & Prado Junior 1974) was isolated from Triatoma infestans in Vitichi (Bolivia) and it causes high parasitaemias and moderate mortality in mice. RAL strain (Ribeiro et al 1993) was also isolated from T. infestans in São Paulo (Brazil).…”

“…Nevertheless, the behaviour is not related to mortality; so, while GM kills 100% of experimentally infected mice, Bolivia only causes death of the 83% of the animals (Atienza 1994). Virulence seems to be an intrinsic property of each strain (Kagan et al 1966, Barbosa et al 1988; in fact, some strains usually maintained in laboratories are characterized as strains with high pathogenicity and low parasitaemias (Y strain) or strains with low pathogenicity and high parasitaemias (Bolivia) (Silva & Nusenzweig 1953, Funayama 1974. In the same way as pathogenicity is not related to parasitaemia, the infectivity to cultured mammalian cells could not be a consequence of the natural pathogenicity of a strain (Atienza 1994).…”

Relationship between biological behaviour and randomly amplified polymorphic DNA profiles of Trypanosoma cruzi strains

“…Parasites and DNA preparation -Four strains of T. cruzi were used to DNA extraction: Bolivia strain (Funayama & Prado Junior 1974), RAL strain (Ribeiro et al 1993), GM strain (Prado Junior et al 1992a) and MC strain (Prado Junior et al 1992b). After infecting groups of NMRI mice by the intraperitoneal route, samples of blood obtained by cardiac puncture were cultured in glass tubes with 5 ml LIT medium (10% heat-inactivated fetal calf serum) and two subcultures were made before beginning the assays.…”

Estudio Cariométrico de Placentas de Ratones con Infección Aguda por Diferentes Cepas de Trypanosoma cruzi

“…Grupo I -Inyectado el DP10 con suero fisiológico estéril (grupo control); Grupo II -Inoculado el DP13 con la cepa colombiana del T. cruzi (Federici et al, 1964), Grupo III -Inoculado el DP13 con la cepa Y del T. cruzi (Silva & Nussenzweig, 1953), Grupo IV -Inoculado el DP6 con la cepa Bolivia del T. Cruzi (Funayama & Prado, 1974), y Grupo V -Inoculado el DP8 con la cepa RC del T. cruzi (Albuquerque & Barreto, 1968 Los animales fueron sacrificados en el DP19, por dislocación cervical bajo anestesia con tribromoetanol. Los fetos y anexos fueron retirados y rápidamente fijados en alcohol 80% -85 mL, formol 10 mL y ácido acético -5 mL, por 24 h.…”