Imunização de camundongos com «vacina» viva avirulenta de Trypanosoma cruzi

Menezes, H

doi:10.1590/s0037-86821969000600001

Cited by 4 publications

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“…In a previous paper, as already m entioned, I dem onstrated th a t subcutaneous injection of 3 x 10-parasites of the PF strain was able to induce a n efficient pro tection in mice against a challenge th a t killed 80% of the control mice (14).…”

Section: Discussionmentioning

confidence: 89%

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The un-infectivity of the PF cultivated strain of trypanosoma cruzi to mice. An evaluation through a one year period by blood cultures and histopathology

Menezes¹

1975

Rev. Soc. Bras. Med. Trop.

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Trypanosoma cruzi of the cultivated PF strain w hen injecteã in mice per subcutaneous route, in aâequate doses, is able to induce an, efficient sterile im m unization in the animais (for at least one year) as determ ined by whole blood cultures and histopathology. IN T R O D U C T IO NIt is well known th a t each antigen presented to th e lymphoid system can react with a population of im munocompetent cells, variable with the antigen used (4).The quantity and th e route used for the antigen are also of im portance, giving place to an im mune response which may vary from optimal to no response a t ali (im m unotolerant condition).I h ad dem onstrated (14) th a t 3 x 102 live parasites of the PF strain injected by subcutaneous route, with about 30 trypomastigotes, were able to give an efficient protection to lOg body weight mice, against a virulent infection of Trypanosoma cruzi th a t killed 80% of th e control animais.This work aims to dem onstrate th a t the route and th e dose of the live PF parasi tes are very im portant in obtaining a safe immune response, as applicable to any live vaccine. MATERIAL AND METHODSAlbino mice from our Medicai School Animal Facilities were used. 130 of them, ali male, with an initial body weight of lOg were divided in groups of 10. 80 of these anim ais received by sub cutaneous route 6 x 103 parasites of the PF strain raised in W arren médium (22), dialyzated in accordance with the Nakam ura technique (20).The culture médium was put into cellophane bags immersed in 1.5 volumes of R inger-H artm ann solution (10) adjusted w ith NaOH sol. to pH 7.5.The culture used as vaccine was 8 days old and the percentage of mobile forms was about 80% with 0.8% trypomastigotes, as determ ined in 1,000 parasites counted a t rantíom in Giemsa stained smears.The Ringer solution with flagellates was centrifuged a t 1,500 rpm for 15 minutes and the sedim ent suspended in sterile so-* P r e s e n te d t o t h e X C o n g re ss o B ra s ile ir o d e P a to lo g ia . C iir itib a , 05/09/74. ** U S P . D e p a r ta m e n to d e G e n é ti c a e M a te m á tic a A p lic a d a à B io lo g ia d a F a c u ld a d e d e M e d ic in a d e R i b e ir ã o P r e to , S á o P a u lo , 14.100, B ra s il. S u b m i tte d to p u b lic a tio n o n 12-6-1974.

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Section: Discussionmentioning

confidence: 89%

“…I h ad dem onstrated (14) th a t 3 x 102 live parasites of the PF strain injected by subcutaneous route, with about 30 trypomastigotes, were able to give an efficient protection to lOg body weight mice, against a virulent infection of Trypanosoma cruzi th a t killed 80% of th e control animais.…”

Section: Introductionmentioning

confidence: 99%

The un-infectivity of the PF cultivated strain of trypanosoma cruzi to mice. An evaluation through a one year period by blood cultures and histopathology

Menezes¹

1975

Rev. Soc. Bras. Med. Trop.

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Trypanosoma cruzi: properties of a clone isolated from CL strain

et al. 1991

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BALB/c mice injected i.p. with 2 x 10(6) metacyclic forms of CL-14, a clone isolated from the CL strain of Trypanosoma cruzi, did not show parasitemia as evaluated by direct blood microscopy examination, hemoculture and xenodiagnosis. Moreover, new-born mice (1-2 days old) injected with culture- or insect-derived CL-14 trypomastigotes also displayed negative parasitemia. No mortality was observed in either group of animals. However, despite this apparent non-infectivity, mice injected with clone 14 developed high resistance against a lethal challenge with virulent trypomastigotes. All challenged mice survived and the parasitemia was negative. These results indicate that clone 14 is a very good antigen for the study of acquired immunity in T. cruzi infection and, therefore, a potential candidate for the development of a vaccine against this parasite.

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The avirulence of the cultivated PF strain of Trypanosoma cruzi: V - the evaluation of parasitologic tests after vaccination of different animal species

Menezes¹,

Ribeiro²

1972

Rev. Soc. Bras. Med. Trop.

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A fter vaccin atio n w ith th e live P F stra in of T rypanosom a cruzi, 194 blood cultures were perform ed in 143 mice, 9 dogs, 5 Cebus m onkeys and 7 h u m a n subjects.Some of these blood cultures were sim ultaneously done w ith xenodiagnosis, subinoculation in baby mice a n d /o r cu ltu re of viscerae.The trypanosom es isolated from th e few positive cases (6,1% ) were in capable of infecting baby mice were considered as cases of im m unotolerance. All th e oth er tests were negative.This work intends to d em o n strate th ro u g h blood cultures, xenodiagnosis, sub inoculations an d culture of viscerae, th e avirulence of th e P F stra in of T rypano som a cruzi (20) in laboratory anim als and h u m an s (6,8,12,18) . MATERIAL AND METHODSThe m ajo rity of anim als described in th is com m unication have already been used in several experim ents perform ed in th e last four years by one of us (6 -2 0 ), a com m on face being th a t all of th em h a d been vaccinated w ith th e PF stra in w ith th e doses m entioned in Table 1.They la te r h a d blood culture w ith or w ithout one or several of th e tests specified above.164 anim als w ere used; 143 w hite mice, 9 dogs, 5 Cebus m onkeys an d 7 h u m an beings in w hich 194 blood cultures were p e rfo rm e d .The culture m edium used was th a t of W arren, as described by one of us (1 ) . T he tests were realized 8,15,20, 30, 45, 60, 90, 120, 150, 180, 270 an d 510 days a fte r vac cin atio n . In some groups tests w ere m ade only once in one of these periods b u t in o thers in more th a n one period or in all of th em as was th e case w ith th e h u m an subjects.Among th e mice th a n h a d only blood cultures, th e am ount of to ta l blood or se ru m used was 0,4 to 0,7 ml for each test tube of culture m edium .W hen more th a n one te st was p e r form ed on mice, th a t am ount was only 0,2 m l. In h u m a n subjects, dogs and m on keys, th e q u an tity of to ta l blood or blood serum (th e la te r in accordance w ith S tro u t's tech n iq u e-23) was always 0,5 m l for each te st tube.T he resu lts of th e blood cultures were always recorded on th e 30th. day as a blind te s t since th e tech n ician h a d no knowledge of the origin of th e m aterial u n d er ex am ination.The xenodiagnosis w ad done by th e use of 4th. in sta r nym phes of R hodnius * U S P . F a c u ld a d e d e M ed icin a de R ib e irã o P rê to . S ão P a u lo . B ra sil. ** D e p a rta m e n to d e C iê n c ia s B io ló gicas. F a c . F a r . O d o n t. R ib e irã o P rê to .R ecebid o p a ra p u b lic a ç ã o em 14-12-1971.

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Imunização de camundongos com «vacina» viva avirulenta de Trypanosoma cruzi

Cited by 4 publications

References 2 publications

The un-infectivity of the PF cultivated strain of trypanosoma cruzi to mice. An evaluation through a one year period by blood cultures and histopathology

The un-infectivity of the PF cultivated strain of trypanosoma cruzi to mice. An evaluation through a one year period by blood cultures and histopathology

Trypanosoma cruzi: properties of a clone isolated from CL strain

The avirulence of the cultivated PF strain of Trypanosoma cruzi: V - the evaluation of parasitologic tests after vaccination of different animal species

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