The effect of phenolated "vaccines" against experimental T. cruzi infection in mice

Abstract

"Vaccines" prepared from parasites of an avirulent cultivated Y strain of T. cruzi, suspended in phenolated 1/10.000 saline solution, with aluminum stearate, containing alive parasites, gave high degree of protection to mice against a posterior infection with virulent blood forms of the same parasites and strain. The degree of protection with 1/1000 and 1/10.000 phenol "vaccines", with no alive parasites, was very poor specially in the first group. The immunity seems to be related to the number of alive trypanosomes in the "vaccines".

Immunization Against Experimental Chagas' Disease by Using Culture Forms of Trypanosoma cruzi Killed with a Solution of Sodium Perchlorate

“…Based on this concept and considering the possible risks involved in immunization with live flagellates (1), several attempts have been made to prepare an effective immunogen with killed organisms. The use of parasites killed by either chemical (10,13,14) or some physical (11) means has usually yielded unsuccessful results, due perhaps to the aggresiveness of the reagents or procedures on the antigens. However, partial protection has been reported achieved with culture forms of T. cruzi killed by some physical methods (F. C. Goble, J. L. Boyd, M. G. Wehner, and M. Konrath, J. Parasitol.…”