IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES

Abstract

SUMMARYThe aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

Screening of Strongyloides infection using an ELISA test in transplant candidates

“…Antigenic fractions were obtained according to well-standardized methods 9. Saline extracts of S. venezuelensis filariform larvae (L3) were obtained from charcoal cultures of feces from experimentally infected Rattus norvegicus (Wistar), protocol (CPE-IMT 2011/126).…”

“…As described previously 9, polystyrene microplates were coated with each of the antigenic fractions (SS, TS, SM, and TM) at concentrations of 10 μg/mL in carbonate-bicarbonate buffer (0.06 M, pH 9.6) prior to incubation overnight at 4°C. After washing with PBS containing 0.05% Tween-20 and 3% nonfat milk (PBS-TM), the microplates were incubated with serum samples diluted 1:200 PBS-TM for 45 min at 37°C.…”

“…Thus, serological techniques have been used as alternative diagnostic tools, demonstrating higher sensitivity than that of parasitological methods 7. In a previous study by our group 9, IgG-ELISA using filariform larvae antigens of Strongyloides venezuelensis showed 90-100% sensitivity and 92.4-98.4% specificity.…”

P a n g a s t r i t i s b y S t r o n g y l o i d e s s t e r c o r a l i s i n A n Immunocompetent Patient: Case Report and Literature Review

“…Also, they are not capable of distinguishing acute and chronic infections [1,8] . Some alternative diagnostic tools (antigens and molecular diagnosis) have been proposed, but they are not available for clinical use in many hospitals [12,13] . The endoscopic findings are usually nonspecific and may show thickened gastric folds, mucosal erosions and ulcers [1,9,10] .…”

Imunoproteômica aplicada ao aprimoramento do diagnóstico sorológico da estrongiloidíase humana

“…Foram utilizadas 69 amostras de soro armazenadas no Laboratório de Investigação Médica (LIM-06), de Imunopatologia da Esquistossomose e outras parasitoses, provenientes de outros trabalhos realizados pelo grupo (Corral et al, 2015a(Corral et al, , 2015b As amostras de fezes foram processadas imediatamente após a coleta pelos métodos de sedimentação, cultura em placa de ágar e Rugai. Para técnica de sedimentação espontânea (Lutz, 1919), aproximadamente 5g de fezes foram diluídos em água filtrada, sendo posteriormente filtrados em gaze, permanecendo em repouso por aproximadamente duas horas.…”