2014
DOI: 10.1590/s0036-46652014000500014
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False-Negative Dengue Cases in Roraima, Brazil: An Approach Regarding the High Number of Negative Results by Ns1 Ag Kits

Abstract: Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out of 150 NS1-antigen negative samples. Of these, 72% were collected up to day 2 after the onset of symptoms, when diagn… Show more

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Cited by 16 publications
(17 citation statements)
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References 17 publications
(23 reference statements)
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“…Since IgA also appears in the body fluids during the same time period as IgM, the combination of NS1 Ag and IgA antibody-based tests can serve as an alternative. NS1 Ag-based tests also had lower sensitivity during secondary infections [3][4][5]. NS1 Ag-based tests also had lower sensitivity during secondary infections [3][4][5].…”
Section: Infection Statusmentioning
confidence: 95%
See 1 more Smart Citation
“…Since IgA also appears in the body fluids during the same time period as IgM, the combination of NS1 Ag and IgA antibody-based tests can serve as an alternative. NS1 Ag-based tests also had lower sensitivity during secondary infections [3][4][5]. NS1 Ag-based tests also had lower sensitivity during secondary infections [3][4][5].…”
Section: Infection Statusmentioning
confidence: 95%
“…However, recent studies have indicated that the sensitivity of NS1 antigen-based tests are lower in secondary infections and in DENV-4 serotype infections [3][4][5]. Nucleic acid-based tests offer the best opportunity for early diagnosis of dengue but are expensive and not available in resource-limited settings.…”
Section: Introductionmentioning
confidence: 99%
“…Hence Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection. 7 Similar study found Underreporting of dengue-4 in Brazil due to low sensitivity of the NS1 Ag test in routine control programs. In a study by Felix AC et al total of 379 RNA-positive samples were selected for thorough evaluation.…”
Section: Resultsmentioning
confidence: 72%
“…Investigadores de Brasil disminuyeron la negatividad en un 10% utilizando técnicas moleculares al considerar a pruebas NS1 negativas como las falsa negativas. Esta situación debería valorarse para mejorar la vigilancia de las arbovirosis 25 .…”
Section: Discussionunclassified