Anopheles (Kerteszia) cruzii (DIPTERA: CULICIDAE) IN PERIDOMICILIARY AREA DURING ASYMPTOMATIC MALARIA TRANSMISSION IN THE ATLANTIC FOREST: MOLECULAR IDENTIFICATION OF BLOOD-MEAL SOURCES INDICATES HUMANS AS PRIMARY INTERMEDIATE HOSTS

Kirchgatter, Karin; Tubaki, Rosa Maria; Malafronte, Rosely dos Santos; Alves, Isabel Cristina; Lima, Giselle Fernandes Maciel de Castro; Guimarães, Lilian de Oliveira; Zampaulo, Robson de Almeida; Wunderlich, Gerd

doi:10.1590/s0036-46652014000500006

Cited by 29 publications

(20 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Their clustering in the phylogenetic tree, with the human isolate from Africa could indicate its origin and also would explain why many of these infections are symptomatic [ 35 ]. On the other hand, for the Atlantic forest region, the clustering of sequences from human isolates with sequences from simian isolates supports the idea of zoonotic malaria in this region [ 7 ], although it is very likely that it is being currently maintained in the region due to asymptomatic individuals who are acting as main source of parasites [ 36 ].…”

Section: Discussionmentioning

confidence: 94%

Merozoite surface protein-1 genetic diversity in Plasmodium malariae and Plasmodium brasilianum from Brazil

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundThe merozoite surface protein 1 (MSP1) gene encodes the major surface antigen of invasive forms of the Plasmodium erythrocytic stages and is considered a candidate vaccine antigen against malaria. Due to its polymorphisms, MSP1 is also useful for strain discrimination and consists of a good genetic marker. Sequence diversity in MSP1 has been analyzed in field isolates of three human parasites: P. falciparum, P. vivax, and P. ovale. However, the extent of variation in another human parasite, P. malariae, remains unknown. This parasite shows widespread, uneven distribution in tropical and subtropical regions throughout South America, Asia, and Africa. Interestingly, it is genetically indistinguishable from P. brasilianum, a parasite known to infect New World monkeys in Central and South America.MethodsSpecific fragments (1 to 5) covering 60 % of the MSP1 gene (mainly the putatively polymorphic regions), were amplified by PCR in isolates of P. malariae and P. brasilianum from different geographic origin and hosts. Sequencing of the PCR-amplified products or cloned PCR fragments was performed and the sequences were used to construct a phylogenetic tree by the maximum likelihood method. Data were computed to give insights into the evolutionary and phylogenetic relationships of these parasites.ResultsExcept for fragment 4, sequences from all other fragments consisted of unpublished sequences. The most polymorphic gene region was fragment 2, and in samples where this region lacks polymorphism, all other regions are also identical. The low variability of the P. malariae msp1 sequences of these isolates and the identification of the same haplotype in those collected many years apart at different locations is compatible with a low transmission rate. We also found greater diversity among P. brasilianum isolates compared with P. malariae ones. Lastly, the sequences were segregated according to their geographic origins and hosts, showing a strong genetic and geographic structure.ConclusionsOur data show that there is a low level of sequence diversity and a possible absence of allelic dimorphism of MSP1 in these parasites as opposed to other Plasmodium species. P. brasilianum strains apparently show greater divergence in comparison to P. malariae, thus P. malariae could derive from P. brasilianum, as it has been proposed.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-015-1238-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 94%

Merozoite surface protein-1 genetic diversity in Plasmodium malariae and Plasmodium brasilianum from Brazil

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…This hypothesis has gained greater support in recent years from studies that have proven the role of NHPs as reservoirs of P. simium and the finding of infected humans in close proximity to forests where the reservoir and vector are present [2,[6][7][8]11]. The second hypothesis, which does not exclude the first, is that asymptomatic human infections are responsible for maintaining transmission foci [49] and that alternative vector species may participate in the transmission cycle in areas where An. cruzii is less abundant [50].…”

Section: Discussionmentioning

confidence: 99%

“…More recent studies using molecular techniques indicate a prevalence of 25 to 35 % in howler monkeys and a minimum infection rate of 0.01 to 1 % in An. cruzii [11,33,49,50,52,58,59]. A prevalence of around 2 to 3 % has been observed in human populations tested in areas of Southeastern Brazil [5][6][7].…”

Section: Discussionmentioning

confidence: 99%

A mathematical model for zoonotic transmission of malaria in the Atlantic Forest: exploring the effects of variations in vector abundance and acrodendrophily

Sousa

Laporta²,

Coutinho³

et al. 2020

Preprint

View full text Add to dashboard Cite

Transmission foci of autochthonous malaria caused by Plasmodium vivax -like parasites have frequently been reported in the Atlantic Forest in Southeastern and Southern Brazil. Evidence suggests that malaria is a zoonosis in these areas as human infections by simian Plasmodium species have been detected, and the main vector of malaria in the Atlantic Forest, Anopheles ( Kerteszia ) cruzii , can blood feed on human and simian hosts. In view of the lack of models that seek to predict the dynamics of zoonotic transmission in this part of the Atlantic Forest, the present study proposes a new deterministic mathematical model that includes a transmission compartment for non-human primates and parameters that take into account vector displacement between the upper and lower forest strata. The effects of variations in the abundance and acrodendrophily of An. cruzii on the prevalence of infected humans in the study area and the basic reproduction number (R 0 ) for malaria were analyzed. The model parameters are based on the literature and fitting of the empirical data. Simulations performed with the model indicate that (1) an increase in the abundance of the vector in relation to the total number of blood-seeking mosquitoes leads to an asymptotic increase in both the proportion of infected individuals at steady state and R 0 ; (2) the proportion of infected humans at steady state is higher when displacement of the vector mosquito between the forest strata increases; and (3) in most scenarios, Plasmodium transmission cannot be sustained only between mosquitoes and humans, which implies that non-human primates play an important role in maintaining the transmission cycle. The proposed model contributes to a better understanding of the dynamics of malaria transmission in the Atlantic Forest.

show abstract

“…O nome malária-bromélias é devido ao local em que as formas imaturas dessa espécie se desenvolvem, em bromélia epífitas e de solo, quase sempre protegidas dos raios solares (Deane et al, 1970;Consoli & Lourenço-deOliveira, 1994;Forattini, 2002;Kirchgatter et al, 2014). Um alto número de indivíduos desta espécie já havia sido coletado na APA Capivari-Monos por Ribeiro e colaboradores (2012), que salientaram a importância da espécie no ciclo da malária nessa área, pela proximidade com humanos e ocorrência em ambiente antrópico para repasto sanguíneo (Forattini et al, 2000).…”

Section: Da Espécieunclassified

Estudo da resposta de mosquitos (Diptera:Culicidae) a diferentes tipos de criadouros e condições de fatores físico-químicos da água

Camargo¹

View full text Add to dashboard Cite

Autorizo a reprodução e divulgação total ou parcial deste trabalho, por qualquer meio convencional ou eletrônico, para fins de estudo e pesquisa, desde que citada a fonte. Agradeço aos meus pais, Nino e Solange, que sempre foram minha base e me deram todo o apoio necessário para chegar até aqui. Sem eles nada disso seria possível. Agradeço também minha irmã Samantha, por ser sempre 6 a melhor irmã e companheira. Agradeço à minha avó Maria Dolores, por ser também minha base e em quem eu me espelho todos os dias. E a todos da minha família que mesmo que pouquinho, me ajudaram a chegar até aqui. E agradeço à Caroline, meu amor, que me inspira a ser alguém melhor todos os dias.Muito obrigada a todos que de alguma forma contribuíram para que esse trabalho fosse possível! 7 Resumo Camargo, A.A. Estudo da resposta de mosquitos (Diptera:Culicidae) a diferentes tipos de criadouros e condições de fatores físico-químicos da água.[dissertação]. São Paulo: Faculdade de Saúde Pública da USP; 2018.A família dos culicídeos é diversa e possui muitas espécies com alta relevância epidemiológica e que transmitem os patógenos causadores de doenças como dengue, febre amarela e malária. Os imaturos se criam exclusivamente na água e nos mais diversos tipos de criadouros, podendo ser influenciados por fatores biológicos e fatores físico-químicos, que incluem pH, salinidade, condutividade, sólidos totais dissolvidos, entre outros. Entender as variações das condições dos criadouros é importante para entender e esclarecer como esses fatores agem no desenvolvimento dos imaturos, na sua fisiologia e nas relações interespecíficas, que influenciam o desenvolvimento larval e a sobrevivência dos mosquitos. Portanto, o objetivo principal desse trabalho foi investigar a influência de fatores físico-químicos e do tipo de criadouro sobre a ocorrência das espécies de culicídeos em dois remanescentes de Mata Atlântica no município de São Paulo. As coletas dos imaturos ocorreram em três diferentes pontos no Parque Estadual da Cantareira e quatro diferentes pontos na Área de Proteção Ambiental CapivariMonos, remanescentes de Mata Atlântica inseridos na Grande São Paulo, durante vinte e sete meses para as coletas de diversidade geral e doze meses para as coletas de diversidade juntamente com fatores físico químicos da água. Os imaturos foram coletados em bromélias epífitas e de solo, bambus, poças d'água no solo e em pedras, ocos de árvore, lagos e recipientes artificiais. As análises dos dados foram feitas através do cálculo do coeficiente de variação das variáveis para os tipos de criadouros, Análise Restrita de Proximidade (CAP), Análise de Correspondência Canônica (CCA) e boxplots sem outliers. Os resultados indicaram que há uma diferença estatisticamente significante na composição de espécies em função dos diferentes tipos de criadouros e que o pH possui influência sobre a ocorrência das espécies, tanto para a variável tipo de criadouro quanto para situações em que se retira essa variável. As espécies com importância epidemiológica coletadas foram: ...

show abstract

Anopheles (Kerteszia) cruzii (DIPTERA: CULICIDAE) IN PERIDOMICILIARY AREA DURING ASYMPTOMATIC MALARIA TRANSMISSION IN THE ATLANTIC FOREST: MOLECULAR IDENTIFICATION OF BLOOD-MEAL SOURCES INDICATES HUMANS AS PRIMARY INTERMEDIATE HOSTS

Cited by 29 publications

References 31 publications

Merozoite surface protein-1 genetic diversity in Plasmodium malariae and Plasmodium brasilianum from Brazil

Merozoite surface protein-1 genetic diversity in Plasmodium malariae and Plasmodium brasilianum from Brazil

A mathematical model for zoonotic transmission of malaria in the Atlantic Forest: exploring the effects of variations in vector abundance and acrodendrophily

Estudo da resposta de mosquitos (Diptera:Culicidae) a diferentes tipos de criadouros e condições de fatores físico-químicos da água

Contact Info

Product

Resources

About