Establishing the Reference Range for T Lymphocytes Subpopulations in Adults and Children From Brazil

Torres, Alex José Leite; Angelo, Ana Luiza Dias; Silva, Márcio Oliveira; Bastos, Milena de Carvalho; Souza, Denise Ferreira de; Inocêncio, Lílian do Amaral; Lemos, José Alexandre Rodrigues de; Júnior, Ruy de Souza Lino; Castro, Andréa Cauduro de; Palma, Paulo César Rodrigues; Ceci, Loredana; Netto, Eduardo Martins; Brites, Carlos

doi:10.1590/s0036-46652013000500005

Cited by 18 publications

(24 citation statements)

References 24 publications

(26 reference statements)

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“…Our results show that throughout the preoperative period, nutrition and immune parameters remained stable and that after surgery there was an increase in the CD4:CD8 ratio. CD4 and CD8 levels were lower than those reported in the literature for healthy individuals (42%–44% and 24%–32%, respectively) 26 , 27 . However, our results were similar to those seen by others, such as Zhang et al, who assessed the immune profile of 82 patients with GC in different stages of the disease.…”

Section: Discussionsupporting

confidence: 88%

Nutrition and Immune‐Modulatory Intervention in Surgical Patients With Gastric Cancer

et al. 2016

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This study evaluated the effect of an immune-modulatory diet on patients with gastric cancer and identified the parameters associated with postoperative outcomes. This was a single-arm prospective intervention study. At baseline, patients were assessed for nutrition (Patient-Generated Subjective Global Assessment), inflammatory markers (albumin, C-reactive protein, and interleukin 6 [IL-6]), and immune markers (percentage NK, CD4, CD8, and CD4:CD8 ratio); they also received nutrition counseling and high-calorie/protein supplement. A week before surgery, they were assessed for nutrition and inflammatory/immune markers and started on an immune-modulatory supplement until the day before surgery, when they were evaluated again. On the second postoperative day, patients were assessed for inflammatory/immune parameters, and a final nutrition evaluation was performed until the day of discharge. Complications were recorded daily and up to 30 days after discharge. Thirty-seven patients (60 ± 10 years old) were included, and 57% were classified as malnourished. Maintenance of nutrition and immune parameters occurred throughout the study period, but we found a preoperative increase in C-reactive protein (0.1-1.5 mg/dL) and IL-6 (2.0-14.2 pg/mL) and a postoperative increase in the CD4:CD8 ratio (2.3 ± 1.0). Complications and death were seen in 35%, especially patients with higher preoperative IL-6 (2.2-46 pg/mL), lower CD4:CD8 ratio (1.7 ± 0.5), and lower protein (1.2 ± 0.5 g/kg/d) and calorie intake (1552 ± 584 kcal/kg/d). The high-calorie/protein supplementation with the immune-modulating diet was able to maintain the nutrition and immune status of patients with gastric cancer.

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Section: Discussionsupporting

confidence: 88%

Nutrition and Immune‐Modulatory Intervention in Surgical Patients With Gastric Cancer

et al. 2016

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“…The study confirmed the existence and general pathways of age‐related dynamic changes of main Ly subsets, earlier described for normal cohorts . Like in normal children, absolute counts of all subsets in paediatric patients gradually decreased with age.…”

Section: Discussionsupporting

confidence: 87%

“…Surprisingly, although different relative and absolute total Ly count in boys and girls is a well‐established phenomenon, only fragmentary and sometimes contradictory data on gender‐related variations in children have been published. Higher relative and absolute T and T4 cell counts in females of all ages were found in Brazilian population, higher T4 cell count was reported in African American and Hispanic adolescent girls in the United States; on the other hand, no statistical differences were found in an adolescent cohort from South Florida or in children born from HIV‐infected mothers …”

Section: Introductionmentioning

confidence: 80%

Significant age‐ and gender‐related variability of main lymphocyte subsets in paediatric patients: Latvian data

et al. 2018

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Age and gender-related variability of main lymphocyte subsets (T, B and NK cell absolute counts and percentages from Ly; T4, T8 and DN cell absolute counts and percentages from lymphocytes and from T cells; T4:T8 and T:B ratios) was studied in a large cohort of pediatric patients (2 days - 17 years) at yearly intervals. 4128 6-color TBNK tests performed on BD FACSCanto II flow cytometer were assessed; patients with immune deficiencies and tumors were not included. The study revealed significant age and gender-related changes in all subsets. Absolute counts of T, B, T4 cells dropped from neonates to adolescents, decrease of T8 and NK cells was milder; relative count of T cells increased with age and that of B cells decreased; T4:T8 ratio went down and T:B ratio grew. Total T, T4 cells and T4:T8 ratio were significantly higher in girls, while T8, NK and DN cells - in boys; significantly higher relative and absolute B cell counts in boys appeared in adolescence. We compared our results with reference values for healthy children (Tosato et al., 2015), there was a good concordance, except for DN cells. Advantages of using patient cohort instead of healthy children as reference, possibilities for adjusting age and gender-specific reference ranges and potential international data pooling are discussed. This article is protected by copyright. All rights reserved.

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“…Lymphocyte subset distributions by age are reported in Table 1 (percentages) and Table 2 (absolute counts 310 9 /L), which also list WBC and lymphocyte values. We tabulated the 10th and 90th percentiles, in addition to 11 (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18) 11 (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) 11 (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) …”

Section: Resultsmentioning

confidence: 99%

“…Changes in antibody production are the hallmark of various human B-cell deficiencies, known as primary antibody deficiencies (PADs); these conditions are the most common primary immunodeficiencies (PIDs) (5). Some studies (1,2,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) have demonstrated that age, sex, race, geographic area, and nutritional status can influence variation in Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China lymphocyte subsets, and undernutrition due to insufficient intake of energy and macronutrients and/or due to deficiencies in specific micronutrients can impair the immune system, such as suppressing immune functions that are fundamental to host protection (20), which could in turn influence the distribution of different lymphocyte subsets. Normally, the immune system is functionally immature at birth, and it undergoes sequential development that is genetically determined and additionally stimulated by antigen exposure (21).…”

Section: Introductionmentioning

confidence: 99%

Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

Jia

Zhang

et al. 2015

Cytometry Pt A

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Immunophenotyping of blood lymphocytes has become an important tool in the diagnosis of immunologic and hematologic disorders such as immunodeficiencies, lymphoproliferative and autoimmune diseases. Lymphocyte subsets include total T-cells, and NK-cells (CD3 2 CD16 1 CD56 1 ). Specific lymphocyte subset reference intervals should be locally established for meaningful comparison and to obtain an accurate interpretation of the results. Reference intervals of lymphocyte subsets for Chinese children are scarce. We performed dual-platform flow cytometry to determine the reference intervals of the percentages and absolute counts of lymphocyte subsets, including total T-cells, TH cells, TC cells, B-cells, and NK-cells in 1,027 ethnic Han children aged 4 months to 7 years in Henan, China. The children were divided into seven age groups. The percentages and absolute counts differed significantly with age, with the percentages of TH cells and B cells and the CD4/CD8 ratio peaking during the first year, while the percentages of total T cells, TC cells, and NK cells were obviously increased with age; girls showed a trend toward having a higher percentage of TH cells and a higher CD4/CD8 ratio than boys. The absolute counts of lymphocyte subsets peaked during first year and then decreased steadily with age. The reference intervals of lymphocyte subsets among children from China differed from the reported values in Hong Kong, the United States, Cameroon, and Italy. The differences observed could be due to genetic and environmental factors, coupled with the methodology used. The reference intervals of lymphocyte subsets could be used as initial national reference ranges in guidelines for children aged 4 months to 7 years. V C 2015 International Society for Advancement of Cytometry

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Establishing the Reference Range for T Lymphocytes Subpopulations in Adults and Children From Brazil

Cited by 18 publications

References 24 publications

Nutrition and Immune‐Modulatory Intervention in Surgical Patients With Gastric Cancer

Nutrition and Immune‐Modulatory Intervention in Surgical Patients With Gastric Cancer

Significant age‐ and gender‐related variability of main lymphocyte subsets in paediatric patients: Latvian data

Age‐ and sex‐related reference intervals of lymphocyte subsets in healthy ethnic Han Chinese children

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