Effects of vitamin C supplementation on acute phase Chagas disease in experimentally infected mice with Trypanosoma cruzi QM1 strain

Marim, Ricardo Guimarães; Gusmão, Alex Silva de; Castanho, Roberto Esteves Pires; Deminice, Rafael; Therezo, Altino Luiz Silva; Júnior, Alceu Afonso Jordão; Martins, Luciamáre Perinetti Alves

doi:10.1590/s0036-46652012000600006

Cited by 16 publications

(16 citation statements)

References 16 publications

(20 reference statements)

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“…The role of AA in the inflammation and lipid peroxidation during the acute phase of Chagas disease has been studied. In this sense, Marim et al (35), using the Trypanosoma cruzi QM1 strain, showed that AA at the same concentration used in the present work was able to reduce inflammation in cardiac tissue more effectively than a lower dose (0.85 mg/kg). However, this study also showed that AA at 7.14 mg/kg increased the lipid peroxidation levels in the plasma and myocardium of infected mice, evidenced by TBARS levels.…”

Section: Discussionsupporting

confidence: 51%

Benefits of Ascorbic Acid in Association with Low-Dose Benznidazole in Treatment of Chagas Disease

Providello

Carneiro

Portapilla

et al. 2018

Antimicrob Agents Chemother

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The acute phase of Chagas disease (CD) is characterized by high parasitic proliferation and intense inflammation, exacerbating the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). These reactive molecules are also increased by the metabolism of the nitroheterocyclic compounds benznidazole (BZ) and nifurtimox, the only drugs available for the treatment of CD. This oxidative environment, associated with the intracellular multiplication of , leads to tissue destruction, triggering the pathogenic process. Both drugs have limited efficacy and serious side effects, which demonstrates the need to seek alternative therapies. Due to the difficulty in developing new drugs, reviewing therapeutic regimens appears advantageous, and the use of BZ in low doses associated with antioxidants, such as ascorbic acid (AA), would be a valid alternative to attenuate oxidative stress. In our studies, mice receiving the combination of 7.14 mg/kg of body weight/day AA and 10 mg/kg/day BZ10 (AA+BZ10) showed a reduction in parasitemia that was more effective than that with those receiving BZ or AA alone. The combined treatment was effective in decreasing intracellular ROS and lipid peroxidation in cardiac tissue. Histological and PCR analyzes showed that AA also reduced the cardiac parasitism. However, the greatest benefit was seen in AA+BZ10 group, since cardiac inflammation was significantly reduced. In addition, the combined therapy prevented the hepatic damage induced by the infection. Our findings suggest that AA combined with a low dose of BZ may improve the trypanocidal activity and attenuate the toxic effects of BZ. The decrease in oxidative damage and inflammation observed in mice treated with AA+BZ10 could result in increased cardioprotection.

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Section: Discussionsupporting

confidence: 51%

Benefits of Ascorbic Acid in Association with Low-Dose Benznidazole in Treatment of Chagas Disease

Providello

Carneiro

Portapilla

et al. 2018

Antimicrob Agents Chemother

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“…However, in contrast to this lack of an effect of supplementation on the plasma vitamin C level in the acute phase, lower parasitemia levels were detected in the VitC A and VitC/E A groups, although these differences were not statistically significant. Marim et al 30 also observed a decrease in parasitemia levels when administering a dosage equivalent to 500mg of vitamin C once daily to mice infected with the T. cruzi QM1 strain.…”

Section: Discussionmentioning

confidence: 93%

Evaluation of antioxidant therapy in experimental Chagas disease

Tieghi

Manca

Garcia

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Stimulation of inflammatory mediators such as cytokines and chemokines may cause oxidative stress in Chagas disease. In this study, we evaluated the merit of vitamins C and E as antioxidant therapy to minimize the oxidative stress-induced damage in an experimental model of Chagas disease. Methods: Ninety-six Swiss mice were infected with Trypanosoma cruzi QM2 and treated with vitamins C, E, or both (C/E) for 60 and 120 days, and their effects compared to placebo administration were evaluated in the acute and chronic disease phases. Results: There was no difference in parasitemia among treatment groups. However, histological analysis showed more severe inflammation in the skeletal muscle in the vitamin supplementation groups at both the acute and chronic phases. Biochemical analyses during the acute phase showed increased ferric-reducing ability of plasma (FRAP) and glutathione (GSH) levels in the vitamin C and C/E groups. In the chronic phase, a decrease in GSH levels was observed in the vitamin E group and a decrease in thiobarbituric acid reactive substances (TBARS) was observed in the vitamin C/E group. Moreover, there was a decrease in TBARS in the cardiac tissues of the vitamin C and C/E groups compared to that of the placebo group, although this level was greater in the vitamin E group than in the vitamin C group. Conclusions: The antioxidant action of vitamins C and E reduced oxidative stress in both the acute and chronic phases of Chagas disease, with a marked effect from joint administration, indicating their inherent synergism.

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“…Marim et al 5 have recently reported that vitamin C administration reduced parasitemia, although no effect was observed regarding the number of amastigote nets in the other tissues 6 . This phenomenon might limit the effi cacy of the combined treatment in the chronic phase, as other researchers have reported no statistically signifi cant histopathological differences in chronically infected mice that were treated with vitamin C for 180 days 7 .…”

Section: Dear Editormentioning

confidence: 99%

Authors' reply: Vitamins in the treatment of chronic Chagas disease: adjuvant antiparasitary or antioxidant therapy?

Andrade

Oliveira

Nagão-Dias

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Effects of vitamin C supplementation on acute phase Chagas disease in experimentally infected mice with Trypanosoma cruzi QM1 strain

Cited by 16 publications

References 16 publications

Benefits of Ascorbic Acid in Association with Low-Dose Benznidazole in Treatment of Chagas Disease

Benefits of Ascorbic Acid in Association with Low-Dose Benznidazole in Treatment of Chagas Disease

Evaluation of antioxidant therapy in experimental Chagas disease

Authors' reply: Vitamins in the treatment of chronic Chagas disease: adjuvant antiparasitary or antioxidant therapy?

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