Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Abstract

SUMMARYHuman papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6%) were positive for HPV detection, and sixty-three (65.6%) of them showed the presence of at least one HR-HPV. Low-and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3%) multiple infections, 85.7% of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18.

Mutation Detection of E6 and LCR Genes from HPV 16 Associated with Carcinogenesis

“…In a study based on the detection of circulating HPV genotypes in women from Córdoba-Argentina, HPV16 demonstrated to be the most frequent etiological agent (Venezuela et al, 2012).…”

Type-Specific Identification of Genital Human Papillomavirus Infection in Women with Cytological Abnormality

“…However, as reported by Molano et al [7], the viral heterogeneity of this particular cervical condition was no longer seen in severe dysplasia. Thirty percent (60/200) of the women showed negative results for HPV genetic material, probably because the number of viral copies in the sample was below the detection limit of the technique, thus preventing the detection of HPV; maybe the patient's immune response favored the elimination or clearance of the virus [9,12], because the cytological classification did not correspond to the initial diagnosis and those patients had cervical reactive changes generated by other causes.…”

“…It has been proposed that this situation can be due to the concept that in women over 30 years of age the proportion of transient infections is decreased and they develop cell-mediated immunity against viral genotypes [12] or reach stability in terms of their sex partner, which generally occurs in old age [21]. However, these hypotheses have not been elucidated to date [22].…”

“…The HPV cycle induces the proliferation of basal and parabasal cells, leading to the disruption of epithelial differentiation and generating changes in tissue structure and physiology, which contribute to the pathogenesis of cervical cancer [12]. Squamous abnormalities are identified through cervical cytology according to the Bethesda System (2014), and they comprise: atypical squamous cells (ASC), including ASC of undetermined significance (ASC-US), where ASC cannot exclude H-SIL; low-grade squamous intraepithelial lesions, including low-grade dysplasia; high-grade squamous intraepithelial lesions, including moderate and severe dysplasia (CIN II AND CIN III), and squamous cell carcinoma [13].…”

Diagnostic screening of HPV genotypes in 555 Ecuadorian mestizo women of seven provinces, and comparison with other Latino American populations

“…The objective of this study is to show the prevalence of HPV genotypes in Ecuadorian mestizo women [13], in comparison with other studies at Latin America [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] (see table 3).…”