Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009

Bisordi, Ivani; Rocco, Iray Maria; Suzuki, Akemi; Katz, Gizelda; Silveira, Vivian Regina; Maeda, Adriana Yurika; Souza, Renato Pereira de; Bassi, Margarida Georgina; Tedesco, Eloisa Fonseca Del; Freitas, Eithma; Bessa, Thirsa Alvares Franco

doi:10.1590/s0036-46652011000600003

Cited by 10 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accurate diagnosis of dengue can be confirmed based on serum positivity for NS1 antigen, IgM, and IgG antibodies. The NS1 antigen can be determined by ELISA, which is highly sensitive, specific, 12 and helpful in the early diagnosis of acute infection. In our study, we observed that platelet count determination had been used in most (50%) of the cases to diagnose dengue infection.…”

Section: Resultsmentioning

confidence: 99%

Prescribing antibiotics to pediatric dengue: increasing risk of bacterial resistance

Sandopa

Nethi

Sreeram

et al. 2018

View full text Add to dashboard Cite

Background Use of antibiotics to treat self-limiting viral infections like dengue fever (DF) without any co-morbid conditions in pediatric patients is common practice in India, and a major contribution of the inappropriate use of antibiotics in the country.Objective To provide an analysis of diagnosis, grading, and prescribing of antibiotics in pediatric inpatients with DF in a tertiary care teaching hospital in India.Methods Data from case sheets of all pediatric inpatients (n=370) diagnosed with DF without co-morbid conditions were collected with regards to diagnosis, grading, presence, and appropriateness of antibiotic usage according to the 2009 WHO Guidelines, the National Vector Borne Disease Control Program (NVBDCP) of India Guidelines, and the Hospital Infection Society (HIS) Guidelines.Results Platelet count determination (50% of the cases) was the major diagnostic method for dengue. Inappropriate grading of DF was seen in 20% of patients. Almost 75% of the 370 dengue cases were prescribed antibiotics for the expressed purpose of avoiding hospital-acquired infections. A single antibiotic was given in 225 cases (60.81%), 2 antibiotics in 33 (8.91 %) cases, and 3 antibiotics in 9 (2.43%) cases.Conclusions From the results it is clear that antibiotics were prescribed to treat DF where the antibiotics do not have any role. DF is a self-limiting viral infection that can be treated with proper management of hemodynamic status with IV fluids. To avoid the usage of antibiotics in the treatment of dengue, awareness has to be created in healthcare professionals regarding the treatment guidelines for dengue and appropriate use of antibiotics to avoid hospital acquired infections.

show abstract

Section: Resultsmentioning

confidence: 99%

Prescribing antibiotics to pediatric dengue: increasing risk of bacterial resistance

Sandopa

Nethi

Sreeram

et al. 2018

View full text Add to dashboard Cite

show abstract

“…10 It is available in both district and reference centres. 4 According to Bisordi et al, 11 the NS1 assay is highly suitable for samples undergoing screening for virus isolation. Study of Andries et al, 12 also revealed that the discovery of the NS1 protein as an early marker for DENV infection, especially in rapid diagnostic test format now allows dengue diagnosis during the early phase of the disease, even in laboratories with limited equipment and human resources.…”

Section: Resultsmentioning

confidence: 99%

Knowledge of Medical Professionals Towards Dengue Diagnostics

Kamaraj¹,

Sankarlal²,

Mohamed³

et al. 2016

jemds

View full text Add to dashboard Cite

Dengue is a major public health problem throughout the world. It is a rapidly spreading mosquito-borne viral disease with high rate of morbidity and mortality. Dengue can be reduced by implementing early case detection, reorienting health services, improving outbreak prediction and detection through coordinated epidemic and appropriate vector management. Various diagnostic techniques like ELISA, Rapid tests, etc. are very useful in diagnosis of dengue. Diagnosis of dengue is the most essential step to curb any mass outbreak of the disease. OBJECTIVESThe objectives of the study were to find the existing knowledge of Dengue among medical professionals and preference of diagnostic techniques of dengue and to elicit acceptability and affordability of such measures and to highlight the policies regarding dengue diagnostics. METHODA questionnaire survey was conducted among 100 doctors using a pretested open-ended questionnaire. The result was analysed and interpreted. RESULTSThe knowledge of medical professionals regarding the preference of dengue diagnostics varies; 56% of the medical professionals prefer IgM antibody ELISA test, 41% prefer NS I antigen test and only 3% prefer RT -PCR; 100% of the medical professionals agreed that platelet count decreases during the course of infection. An increase of haematocrit value was thought to be important by 73% professionals. Interestingly, 20% of the medical professionals reported that no change in haematocrit value and 7% reported that haematocrit value would decrease. The knowledge of availability of ELISA was 72%. However, 83% of medical professionals agreed that IgM antibody ELISA test was a rapid test for Dengue diagnosis. CONCLUSIONLaboratory infrastructure, technical expertise and research capacity must be improved in order to positively influence dengue surveillance, clinical case management and development of new approaches to dengue control. CME on dengue is suggested to improve the knowledge on diagnostic facilities and its sensitivity, specificity and reliability.

show abstract

“…Moreover, among the studies that were included in this review, only Dos Santos et al, used the ELISA to capture the NS1 antigen in the acute phase of dengue [45]. This method is more sensitive than traditional RT-PCR and is used in many laboratories for the early diagnosis of dengue [65].…”

Section: Discussionmentioning

confidence: 99%

A systematic review of molecular diagnostic methods for the detection of arboviruses in clinical specimens in Brazil and the importance of a differential diagnosis

Moreli¹,

Costa²

2013

Virol Discov

View full text Add to dashboard Cite

Arboviruses (Arthropod-borne viruses) compose a large group of zoonotic viruses that have complex cycles and often cause diseases in humans. This study was aimed at performing a systematic review of molecular diagnostic methods for the detection of arboviruses in clinical samples from Brazil to emphasize the importance of a differential diagnosis. Articles on arbovirus diagnostic methods were searched in databases using descriptors and selection criteria. A total of 19 articles were found that described techniques that may be used for the differential diagnosis of arboviruses. RT-PCR, nested RT-PCR and real-time RT-PCR were the main methods. The samples were collected from the Brazilian Amazon and the state of São Paulo for the diagnosis of dengue, Oropouche fever, yellow fever, Saint Louis encephalitis and Mayaro virus disease. Classical diagnostic methods were rarely used. In addition, molecular methods are not yet fully standardized because several methods did not detect arboviruses. Furthermore, a diagnosis that is based only on clinical and epidemiological data would be premature. Therefore, new entomological research and new differential molecular methods should be performed for the possible isolation of these unknown viruses to contribute to the diagnosis of arboviruses. Thus, additional research should be conducted because these diseases are emerging and reemerging in several countries.

show abstract

Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009

Cited by 10 publications

References 20 publications

Prescribing antibiotics to pediatric dengue: increasing risk of bacterial resistance

Prescribing antibiotics to pediatric dengue: increasing risk of bacterial resistance

Knowledge of Medical Professionals Towards Dengue Diagnostics

A systematic review of molecular diagnostic methods for the detection of arboviruses in clinical specimens in Brazil and the importance of a differential diagnosis

Contact Info

Product

Resources

About